Human neutrophils present mild activation by Zika virus but reduce the infection of susceptible cells

Detalhes bibliográficos
Autor(a) principal: Aggio, Juliana Bernardi
Data de Publicação: 2022
Outros Autores: Porto, Bárbara Nery, Santos, Claudia Nunes Duarte dos, Mosimann, Ana Luiza Pamplona, Wowk, Pryscilla Fanini
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/54662
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
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spelling Aggio, Juliana BernardiPorto, Bárbara NerySantos, Claudia Nunes Duarte dosMosimann, Ana Luiza PamplonaWowk, Pryscilla Fanini2022-08-16T15:55:36Z2022-08-16T15:55:36Z2022AGGIO, Juliana Bernardi et al. Human neutrophils present mild activation by Zika Virus but reduce the infection of susceptible cells. Biomolecules, v. 12, n. 769, p. 1–33, 2022.1664-3224https://www.arca.fiocruz.br/handle/icict/5466210.3389/fimmu.2022.784443porFrontiers MediaHuman neutrophils present mild activation by Zika virus but reduce the infection of susceptible cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Department of Medical Microbiology and Infectious Diseases. University of Manitoba. Winnipeg, MB, Canada. / Biology of Breathing Group. Children’s Hospital Research Institute of Manitoba. Winnipeg, MB, Canada.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.The emergence of the Zika virus (ZIKV) has highlighted the need for a deeper understanding of virus-host interactions in order to pave the way for the development of antiviral therapies. The present work aimed to address the response of neutrophils during ZIKV infection. Neutrophils are important effector cells in innate immunity implicated in the host’s response to neurotropic arboviruses. Our results indicate that human neutrophils were not permissive to Asian or African ZIKV strain replication. In fact, after stimulation with ZIKV, neutrophils were mild primed against the virus as evaluated through CD11b and CD62L modulation, secretion of inflammatory cytokines and granule content, production of reactive oxygen species, and neutrophil extracellular traps formation. Overall, neutrophils did not affect ZIKV infectivity. Moreover, in vitro ZIKV infection of primary innate immune cells did not trigger neutrophil migration. However, neutrophils cocultured with ZIKV susceptible cell lineages resulted in lower cell infection frequencies, possibly due to cell-to-cell contact. In vivo, neutrophil depletion in immunocompetent mice did not affect ZIKV spreading to the draining lymph nodes. The data suggest that human neutrophils do not play an antiviral role against ZIKV per se, but these cells might participate in an infected environment shaping the ZIKV infection in other target cellsNeutrophilsZika VirusImmunity, InnateCoculture TechniquesExtracellular TrapsNeutrófilosVirus ZikaInmunidad InnataTécnicas de CocultivoTrampas ExtracelularesNeutrófilosZika virusImunidade InataTécnicas de CoculturaArmadilhas Extracelularesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/54662/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALfimmu-13-784443ok.pdffimmu-13-784443ok.pdfapplication/pdf9863039https://www.arca.fiocruz.br/bitstream/icict/54662/2/fimmu-13-784443ok.pdf68e00c5e045362689bc5620543cd8f7cMD52icict/546622022-08-16 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dc.title.pt_BR.fl_str_mv Human neutrophils present mild activation by Zika virus but reduce the infection of susceptible cells
title Human neutrophils present mild activation by Zika virus but reduce the infection of susceptible cells
spellingShingle Human neutrophils present mild activation by Zika virus but reduce the infection of susceptible cells
Aggio, Juliana Bernardi
Neutrophils
Zika Virus
Immunity, Innate
Coculture Techniques
Extracellular Traps
Neutrófilos
Virus Zika
Inmunidad Innata
Técnicas de Cocultivo
Trampas Extracelulares
Neutrófilos
Zika virus
Imunidade Inata
Técnicas de Cocultura
Armadilhas Extracelulares
title_short Human neutrophils present mild activation by Zika virus but reduce the infection of susceptible cells
title_full Human neutrophils present mild activation by Zika virus but reduce the infection of susceptible cells
title_fullStr Human neutrophils present mild activation by Zika virus but reduce the infection of susceptible cells
title_full_unstemmed Human neutrophils present mild activation by Zika virus but reduce the infection of susceptible cells
title_sort Human neutrophils present mild activation by Zika virus but reduce the infection of susceptible cells
author Aggio, Juliana Bernardi
author_facet Aggio, Juliana Bernardi
Porto, Bárbara Nery
Santos, Claudia Nunes Duarte dos
Mosimann, Ana Luiza Pamplona
Wowk, Pryscilla Fanini
author_role author
author2 Porto, Bárbara Nery
Santos, Claudia Nunes Duarte dos
Mosimann, Ana Luiza Pamplona
Wowk, Pryscilla Fanini
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Aggio, Juliana Bernardi
Porto, Bárbara Nery
Santos, Claudia Nunes Duarte dos
Mosimann, Ana Luiza Pamplona
Wowk, Pryscilla Fanini
dc.subject.en.pt_BR.fl_str_mv Neutrophils
Zika Virus
Immunity, Innate
Coculture Techniques
Extracellular Traps
topic Neutrophils
Zika Virus
Immunity, Innate
Coculture Techniques
Extracellular Traps
Neutrófilos
Virus Zika
Inmunidad Innata
Técnicas de Cocultivo
Trampas Extracelulares
Neutrófilos
Zika virus
Imunidade Inata
Técnicas de Cocultura
Armadilhas Extracelulares
dc.subject.es.pt_BR.fl_str_mv Neutrófilos
Virus Zika
Inmunidad Innata
Técnicas de Cocultivo
Trampas Extracelulares
dc.subject.decs.pt_BR.fl_str_mv Neutrófilos
Zika virus
Imunidade Inata
Técnicas de Cocultura
Armadilhas Extracelulares
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-08-16T15:55:36Z
dc.date.available.fl_str_mv 2022-08-16T15:55:36Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AGGIO, Juliana Bernardi et al. Human neutrophils present mild activation by Zika Virus but reduce the infection of susceptible cells. Biomolecules, v. 12, n. 769, p. 1–33, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/54662
dc.identifier.issn.pt_BR.fl_str_mv 1664-3224
dc.identifier.doi.none.fl_str_mv 10.3389/fimmu.2022.784443
identifier_str_mv AGGIO, Juliana Bernardi et al. Human neutrophils present mild activation by Zika Virus but reduce the infection of susceptible cells. Biomolecules, v. 12, n. 769, p. 1–33, 2022.
1664-3224
10.3389/fimmu.2022.784443
url https://www.arca.fiocruz.br/handle/icict/54662
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/54662/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/54662/2/fimmu-13-784443ok.pdf
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repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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