Lower airway microbiota and decreasing lung function in young brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infection

Detalhes bibliográficos
Autor(a) principal: Kussek, Paulo
Data de Publicação: 2022
Outros Autores: Mesa, Dany, Vasconcelos, Thaís Muniz, Rodrigues, Luiza Souza, Krul, Damaris, Ibanez, Humberto, Faoro, Helisson, Palmeiro, Jussara Kasuko, Costa, Libera Maria Dalla
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/55509
Resumo: Hospital Pequeno Príncipe. Curitiba, PR, Brasil.
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spelling Kussek, PauloMesa, DanyVasconcelos, Thaís MunizRodrigues, Luiza SouzaKrul, DamarisIbanez, HumbertoFaoro, HelissonPalmeiro, Jussara KasukoCosta, Libera Maria Dalla2022-11-07T17:27:05Z2022-11-07T17:27:05Z2022KUSSEK, Paulo et al. Lower airway microbiota and decreasing lung function in young Brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infection. PLoS ONE, v. 17, n. 8, p. 1–15, 2022.1932-6203https://www.arca.fiocruz.br/handle/icict/5550910.3390/ijms23169175porMDPIStaphylococcusPseudomonasMicrobiotaRespiratory MucosaCystic FibrosisMucosa RespiratoriaFibrosis QuísticaMuqueuse respiratoireMucoviscidoseMucosa RespiratóriaFibrose CísticaLower airway microbiota and decreasing lung function in young brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHospital Pequeno Príncipe. Curitiba, PR, Brasil.Big Data Center. Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba, PR, Brasil. / Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba, PR, Brasil.Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba, PR, Brasil.Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba, PR, Brasil.Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba, PR, Brasil.Big Data Center. Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal de Santa Catarina. Centro de Ciências da Saúde. Departamento de Análises Clínicas. Florianópolis, SC, Brasil.Instituto de Pesquisa Pelé Pequeno Príncipe. Curitiba, PR, Brasil.Cystic fibrosis (CF) is a genetic disease caused by mutations in the cystic fibrosis transmembrane conductance regulator gene that leads to respiratory complications and mortality. Studies have shown shifts in the respiratory microbiota during disease progression in individuals with CF. In addition, CF patients experience short cycles of acute intermittent aggravations of symptoms called pulmonary exacerbations, which may be characterized by a decrease in lung function and weight loss. The resident microbiota become imbalanced, promoting biofilm formation, and reducing the effectiveness of therapy. The aim of this study was to monitor patients aged 8–23 years with CF to evaluate their lower respiratory microbiota using 16S rRNA sequencing. The most predominant pathogens observed in microbiota, Staphylococcus (Staph) and Pseudomonas (Pseud) were correlated with clinical variables, and the in vitro capacity of biofilm formation for these pathogens was tested. A group of 34 patients was followed up for 84 days, and 306 sputum samples were collected and sequenced. Clustering of microbiota by predominant pathogen showed that children with more Staph had reduced forced expiratory volume in one second (FEV1) and forced vital capacity (FVC) compared to children with Pseud. Furthermore, the patients’ clinical condition was consistent with the results of pulmonary function. More patients with pulmonary exacerbation were observed in the Staph group than in the Pseud group, as confirmed by lower body mass index and pulmonary function. Additionally, prediction of bacterial functional profiles identified genes encoding key enzymes involved in virulence pathways in the Pseud group. Importantly, this study is the first Brazilian study to assess the lower respiratory microbiota in a significant group of young CF patients. In this sense, the data collected for this study on the microbiota of children in Brazil with CF provide a valuable contribution to the knowledge in the fieldinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/55509/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjournal.pone.0273453ok.pdfjournal.pone.0273453ok.pdfapplication/pdf1331716https://www.arca.fiocruz.br/bitstream/icict/55509/2/journal.pone.0273453ok.pdf8a8e9d9277dac7ddcdf7a9671583f7ccMD52icict/555092022-11-07 14:27:07.19oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-11-07T17:27:07Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Lower airway microbiota and decreasing lung function in young brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infection
title Lower airway microbiota and decreasing lung function in young brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infection
spellingShingle Lower airway microbiota and decreasing lung function in young brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infection
Kussek, Paulo
Staphylococcus
Pseudomonas
Microbiota
Respiratory Mucosa
Cystic Fibrosis
Mucosa Respiratoria
Fibrosis Quística
Muqueuse respiratoire
Mucoviscidose
Mucosa Respiratória
Fibrose Cística
title_short Lower airway microbiota and decreasing lung function in young brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infection
title_full Lower airway microbiota and decreasing lung function in young brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infection
title_fullStr Lower airway microbiota and decreasing lung function in young brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infection
title_full_unstemmed Lower airway microbiota and decreasing lung function in young brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infection
title_sort Lower airway microbiota and decreasing lung function in young brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infection
author Kussek, Paulo
author_facet Kussek, Paulo
Mesa, Dany
Vasconcelos, Thaís Muniz
Rodrigues, Luiza Souza
Krul, Damaris
Ibanez, Humberto
Faoro, Helisson
Palmeiro, Jussara Kasuko
Costa, Libera Maria Dalla
author_role author
author2 Mesa, Dany
Vasconcelos, Thaís Muniz
Rodrigues, Luiza Souza
Krul, Damaris
Ibanez, Humberto
Faoro, Helisson
Palmeiro, Jussara Kasuko
Costa, Libera Maria Dalla
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Kussek, Paulo
Mesa, Dany
Vasconcelos, Thaís Muniz
Rodrigues, Luiza Souza
Krul, Damaris
Ibanez, Humberto
Faoro, Helisson
Palmeiro, Jussara Kasuko
Costa, Libera Maria Dalla
dc.subject.other.en_US.fl_str_mv Staphylococcus
Pseudomonas
Microbiota
topic Staphylococcus
Pseudomonas
Microbiota
Respiratory Mucosa
Cystic Fibrosis
Mucosa Respiratoria
Fibrosis Quística
Muqueuse respiratoire
Mucoviscidose
Mucosa Respiratória
Fibrose Cística
dc.subject.en.en_US.fl_str_mv Respiratory Mucosa
Cystic Fibrosis
dc.subject.es.en_US.fl_str_mv Mucosa Respiratoria
Fibrosis Quística
dc.subject.fr.en_US.fl_str_mv Muqueuse respiratoire
Mucoviscidose
dc.subject.decs.en_US.fl_str_mv Mucosa Respiratória
Fibrose Cística
description Hospital Pequeno Príncipe. Curitiba, PR, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-11-07T17:27:05Z
dc.date.available.fl_str_mv 2022-11-07T17:27:05Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv KUSSEK, Paulo et al. Lower airway microbiota and decreasing lung function in young Brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infection. PLoS ONE, v. 17, n. 8, p. 1–15, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/55509
dc.identifier.issn.en_US.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.3390/ijms23169175
identifier_str_mv KUSSEK, Paulo et al. Lower airway microbiota and decreasing lung function in young Brazilian cystic fibrosis patients with pulmonary Staphylococcus and Pseudomonas infection. PLoS ONE, v. 17, n. 8, p. 1–15, 2022.
1932-6203
10.3390/ijms23169175
url https://www.arca.fiocruz.br/handle/icict/55509
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/55509/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/55509/2/journal.pone.0273453ok.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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