Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation

Detalhes bibliográficos
Autor(a) principal: Valverde, Ludmila de Faro
Data de Publicação: 2016
Outros Autores: Pereira, Thiago de Almeida, Dias, Rosane Borges, Guimarães, Vanessa Sousa Nazaré, Ramos, Eduardo Antônio Gonçalves, Santos, Jean Nunes, Gurgel Rocha, Clarissa Araújo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17798
Resumo: Ethics approval and consent to participate The present research pro- posal received approval from our host institution’s review board. In- formed consent was obtained from all individual participants included in the study.
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spelling Valverde, Ludmila de FaroPereira, Thiago de AlmeidaDias, Rosane BorgesGuimarães, Vanessa Sousa NazaréRamos, Eduardo Antônio GonçalvesSantos, Jean NunesGurgel Rocha, Clarissa Araújo2017-02-13T13:01:00Z2017-02-13T13:01:00Z2016VALVERDE, L. F. Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation. Tumor Biology, v.37, n.7, p. 9233–9241, 2016.1010-4283https://www.arca.fiocruz.br/handle/icict/1779810.1007/s13277-015-4763-6Ethics approval and consent to participate The present research pro- posal received approval from our host institution’s review board. In- formed consent was obtained from all individual participants included in the study.FAPESB e CNPQFundação Oswaldo Cruz. Centro de Investigação Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Laboratório de Patologia Experimental. Centro de Investigação Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Investigação Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Investigação Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Investigação Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Odontologia. Laboratório de Patologia Cirúrgica Bucal. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Investigação Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Odontologia. Laboratório de Patologia Cirúrgica Bucal. Salvador, BA, Brasil.The present study aimed to evaluate the role of Hedgehog (Hh) molecule expression in association with the clinical aspects of oral squamous cell carcinoma (OSCC), as well as angiogenesis and CD163+ macrophages. Twenty-eight cases of OSCC, nine cases of tumor-free resection margins (TM), and four cases of non-neoplastic oral mucosa (NNM) were submitted to immunohistochemistry to detect proteins Sonic Hedgehog (SHH), Indian Hedgehog (IHH), GLI1, CD163, and CD105. Protein colocalization with respect to SHH/CD163, IHH/CD163, GLI1/CD163, and GLI1/CD105 was assessed by immunohistochemical double staining. In tumor parenchyma, SHH and IHH were present in the cytoplasm of neoplastic cells, while GLI1 was observed in cytoplasm and nucleus. Endothelial cells were found to express SHH, IHH, and GLI1 within CD105+ vessels, and a positive correlation between infiltrating macrophage density (IMD) and microvascular density (MVD) was observed in cases of OSCC and TM. When compared to TM and NNM, the OSCC cases demonstrated higher immunoreactivity for SHH (p = 0.01), IHH (p = 0.39), GLI1 (p = 0.03), IMD (p = 0.0002), and MVD (p = 0.0002). Our results suggest the participation of the Hh pathway in OSCC by way of autocrine and paracrine signaling, in addition to the participation of both SHH and IHH ligands. Endothelial cells were also found to exhibit positivity with respect to Hh pathway components and we surmise that these molecules may play a role in tumor angiogenesis. CD163+ macrophages were also observed to express IHH, a ligand of this pathway, in addition to being associated with tumor neovascularization.engSpringer NatureCâncer oralProteínas HedgehogImuno-HistoquímicaOral cancerSquamous cell carcinomaHedgehog proteinsImmunohistochemistryNeoplasias BucaisProteínas HedgehogImuno-HistoquímicaMacrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation
title Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation
spellingShingle Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation
Valverde, Ludmila de Faro
Câncer oral
Proteínas Hedgehog
Imuno-Histoquímica
Oral cancer
Squamous cell carcinoma
Hedgehog proteins
Immunohistochemistry
Neoplasias Bucais
Proteínas Hedgehog
Imuno-Histoquímica
title_short Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation
title_full Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation
title_fullStr Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation
title_full_unstemmed Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation
title_sort Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation
author Valverde, Ludmila de Faro
author_facet Valverde, Ludmila de Faro
Pereira, Thiago de Almeida
Dias, Rosane Borges
Guimarães, Vanessa Sousa Nazaré
Ramos, Eduardo Antônio Gonçalves
Santos, Jean Nunes
Gurgel Rocha, Clarissa Araújo
author_role author
author2 Pereira, Thiago de Almeida
Dias, Rosane Borges
Guimarães, Vanessa Sousa Nazaré
Ramos, Eduardo Antônio Gonçalves
Santos, Jean Nunes
Gurgel Rocha, Clarissa Araújo
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Valverde, Ludmila de Faro
Pereira, Thiago de Almeida
Dias, Rosane Borges
Guimarães, Vanessa Sousa Nazaré
Ramos, Eduardo Antônio Gonçalves
Santos, Jean Nunes
Gurgel Rocha, Clarissa Araújo
dc.subject.other.pt_BR.fl_str_mv Câncer oral
Proteínas Hedgehog
Imuno-Histoquímica
topic Câncer oral
Proteínas Hedgehog
Imuno-Histoquímica
Oral cancer
Squamous cell carcinoma
Hedgehog proteins
Immunohistochemistry
Neoplasias Bucais
Proteínas Hedgehog
Imuno-Histoquímica
dc.subject.en.pt_BR.fl_str_mv Oral cancer
Squamous cell carcinoma
Hedgehog proteins
Immunohistochemistry
dc.subject.decs.pt_BR.fl_str_mv Neoplasias Bucais
Proteínas Hedgehog
Imuno-Histoquímica
description Ethics approval and consent to participate The present research pro- posal received approval from our host institution’s review board. In- formed consent was obtained from all individual participants included in the study.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-02-13T13:01:00Z
dc.date.available.fl_str_mv 2017-02-13T13:01:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VALVERDE, L. F. Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation. Tumor Biology, v.37, n.7, p. 9233–9241, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17798
dc.identifier.issn.pt_BR.fl_str_mv 1010-4283
dc.identifier.doi.none.fl_str_mv 10.1007/s13277-015-4763-6
identifier_str_mv VALVERDE, L. F. Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation. Tumor Biology, v.37, n.7, p. 9233–9241, 2016.
1010-4283
10.1007/s13277-015-4763-6
url https://www.arca.fiocruz.br/handle/icict/17798
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/17798/1/license.txt
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