Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/17798 |
Resumo: | Ethics approval and consent to participate The present research pro- posal received approval from our host institution’s review board. In- formed consent was obtained from all individual participants included in the study. |
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Valverde, Ludmila de FaroPereira, Thiago de AlmeidaDias, Rosane BorgesGuimarães, Vanessa Sousa NazaréRamos, Eduardo Antônio GonçalvesSantos, Jean NunesGurgel Rocha, Clarissa Araújo2017-02-13T13:01:00Z2017-02-13T13:01:00Z2016VALVERDE, L. F. Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation. Tumor Biology, v.37, n.7, p. 9233–9241, 2016.1010-4283https://www.arca.fiocruz.br/handle/icict/1779810.1007/s13277-015-4763-6Ethics approval and consent to participate The present research pro- posal received approval from our host institution’s review board. In- formed consent was obtained from all individual participants included in the study.FAPESB e CNPQFundação Oswaldo Cruz. Centro de Investigação Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Laboratório de Patologia Experimental. Centro de Investigação Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Investigação Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Investigação Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Investigação Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Odontologia. Laboratório de Patologia Cirúrgica Bucal. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Investigação Gonçalo Moniz. Laboratório de Patologia e Biologia Molecular. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Odontologia. Laboratório de Patologia Cirúrgica Bucal. Salvador, BA, Brasil.The present study aimed to evaluate the role of Hedgehog (Hh) molecule expression in association with the clinical aspects of oral squamous cell carcinoma (OSCC), as well as angiogenesis and CD163+ macrophages. Twenty-eight cases of OSCC, nine cases of tumor-free resection margins (TM), and four cases of non-neoplastic oral mucosa (NNM) were submitted to immunohistochemistry to detect proteins Sonic Hedgehog (SHH), Indian Hedgehog (IHH), GLI1, CD163, and CD105. Protein colocalization with respect to SHH/CD163, IHH/CD163, GLI1/CD163, and GLI1/CD105 was assessed by immunohistochemical double staining. In tumor parenchyma, SHH and IHH were present in the cytoplasm of neoplastic cells, while GLI1 was observed in cytoplasm and nucleus. Endothelial cells were found to express SHH, IHH, and GLI1 within CD105+ vessels, and a positive correlation between infiltrating macrophage density (IMD) and microvascular density (MVD) was observed in cases of OSCC and TM. When compared to TM and NNM, the OSCC cases demonstrated higher immunoreactivity for SHH (p = 0.01), IHH (p = 0.39), GLI1 (p = 0.03), IMD (p = 0.0002), and MVD (p = 0.0002). Our results suggest the participation of the Hh pathway in OSCC by way of autocrine and paracrine signaling, in addition to the participation of both SHH and IHH ligands. Endothelial cells were also found to exhibit positivity with respect to Hh pathway components and we surmise that these molecules may play a role in tumor angiogenesis. CD163+ macrophages were also observed to express IHH, a ligand of this pathway, in addition to being associated with tumor neovascularization.engSpringer NatureCâncer oralProteínas HedgehogImuno-HistoquímicaOral cancerSquamous cell carcinomaHedgehog proteinsImmunohistochemistryNeoplasias BucaisProteínas HedgehogImuno-HistoquímicaMacrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation |
| title |
Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation |
| spellingShingle |
Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation Valverde, Ludmila de Faro Câncer oral Proteínas Hedgehog Imuno-Histoquímica Oral cancer Squamous cell carcinoma Hedgehog proteins Immunohistochemistry Neoplasias Bucais Proteínas Hedgehog Imuno-Histoquímica |
| title_short |
Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation |
| title_full |
Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation |
| title_fullStr |
Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation |
| title_full_unstemmed |
Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation |
| title_sort |
Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation |
| author |
Valverde, Ludmila de Faro |
| author_facet |
Valverde, Ludmila de Faro Pereira, Thiago de Almeida Dias, Rosane Borges Guimarães, Vanessa Sousa Nazaré Ramos, Eduardo Antônio Gonçalves Santos, Jean Nunes Gurgel Rocha, Clarissa Araújo |
| author_role |
author |
| author2 |
Pereira, Thiago de Almeida Dias, Rosane Borges Guimarães, Vanessa Sousa Nazaré Ramos, Eduardo Antônio Gonçalves Santos, Jean Nunes Gurgel Rocha, Clarissa Araújo |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Valverde, Ludmila de Faro Pereira, Thiago de Almeida Dias, Rosane Borges Guimarães, Vanessa Sousa Nazaré Ramos, Eduardo Antônio Gonçalves Santos, Jean Nunes Gurgel Rocha, Clarissa Araújo |
| dc.subject.other.pt_BR.fl_str_mv |
Câncer oral Proteínas Hedgehog Imuno-Histoquímica |
| topic |
Câncer oral Proteínas Hedgehog Imuno-Histoquímica Oral cancer Squamous cell carcinoma Hedgehog proteins Immunohistochemistry Neoplasias Bucais Proteínas Hedgehog Imuno-Histoquímica |
| dc.subject.en.pt_BR.fl_str_mv |
Oral cancer Squamous cell carcinoma Hedgehog proteins Immunohistochemistry |
| dc.subject.decs.pt_BR.fl_str_mv |
Neoplasias Bucais Proteínas Hedgehog Imuno-Histoquímica |
| description |
Ethics approval and consent to participate The present research pro- posal received approval from our host institution’s review board. In- formed consent was obtained from all individual participants included in the study. |
| publishDate |
2016 |
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2016 |
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2017-02-13T13:01:00Z |
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2017-02-13T13:01:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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VALVERDE, L. F. Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation. Tumor Biology, v.37, n.7, p. 9233–9241, 2016. |
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https://www.arca.fiocruz.br/handle/icict/17798 |
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1010-4283 |
| dc.identifier.doi.none.fl_str_mv |
10.1007/s13277-015-4763-6 |
| identifier_str_mv |
VALVERDE, L. F. Macrophages and endothelial cells orchestrate tumor-associated angiogenesis in oral cancer via hedgehog pathway activation. Tumor Biology, v.37, n.7, p. 9233–9241, 2016. 1010-4283 10.1007/s13277-015-4763-6 |
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Springer Nature |
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Springer Nature |
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