Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/35453 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. |
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Barbieri, Raquel R.Manta, Fernanda S. N.Moreira, Suelen J. M.Sales, Anna M.Nery, José A. C.Nascimento, Lilian P. R.Hacker, Mariana A.Pacheco, Antonio G.Machado, Alice M.Sarno, Euzenir M.Moraes, Milton O.2019-09-10T15:33:58Z2019-09-10T15:33:58Z2019BARBIERI, Raquel R. et al. Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study. PLoS Neglected Tropical Diseases, v. 13, n. 3, p.1-12, Mar. 2019.1935-2727https://www.arca.fiocruz.br/handle/icict/3545310.1371/journal.pntd.00071471935-2735engPublic Library of ScienceReação em Cadeia da PolimeraseHanseníaseDiagnósticoCasos suspeitosHistologia inconclusivaPCRSuspected PB casesInconclusive histologyLeprosyDiagnosisQuantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up studyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Presidência. Programa de Computação Científica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.The diagnosis of paucibacillary (PB) leprosy cases remains a challenge because of the absence of a confirmatory laboratory method. While quantitative polymerase chain reaction (qPCR) has been shown to provide reliable sensitivity and specificity in PB diagnoses, a thorough investigation of its efficacy in clinical practice has not yet been published. The present study evaluated patients with suspected leprosy skin lesions by using qPCR to identify PB individuals in the Leprosy Outpatient clinic at the Oswaldo Cruz Foundation in Rio de Janeiro, Brazil. Methods: One hundred seventy-two suspected PB cases were included in the study. The patients were evaluated by a dermatologist at three different times. The clinical dermato-neurological examination and collected samples were performed on the first visit. On the second visit, the results of the histopathological analysis and PCR assay (DNA-based Mycobacterium leprae qPCR-targeting 16S gene) results were analyzed, and a decision regarding multidrug therapy was made. A year later, the patients were re-examined, and the consensus diagnosis was established. Results: In 58% (100/172) of cases, a conclusive diagnosis via histopathological analysis was not possible; however, 30% (30/100) of these cases had a positive PCR. One hundred ten patients (110/172) attended the third visit. The analysis showed that while the sensitivity of the histopathological test was very low (35%), a qPCR alone was more effective for identifying leprosy, with 57% sensitivity. Conclusion: The use of qPCR in suspected PB cases with an inconclusive histology improved the sensitivity of leprosy diagnoses.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35453/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRaquelBarbieri_MiltonOMoraes_etal_IOC_2019.pdfRaquelBarbieri_MiltonOMoraes_etal_IOC_2019.pdfapplication/pdf1300628https://www.arca.fiocruz.br/bitstream/icict/35453/2/RaquelBarbieri_MiltonOMoraes_etal_IOC_2019.pdf8c982cf5b7cf4e69a7cd29cba4fe6075MD52TEXTRaquelBarbieri_MiltonOMoraes_etal_IOC_2019.pdf.txtRaquelBarbieri_MiltonOMoraes_etal_IOC_2019.pdf.txtExtracted texttext/plain35882https://www.arca.fiocruz.br/bitstream/icict/35453/3/RaquelBarbieri_MiltonOMoraes_etal_IOC_2019.pdf.txt00af750690ab90c541fe489c5994e239MD53icict/354532023-09-05 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| dc.title.pt_BR.fl_str_mv |
Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study |
| title |
Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study |
| spellingShingle |
Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study Barbieri, Raquel R. Reação em Cadeia da Polimerase Hanseníase Diagnóstico Casos suspeitos Histologia inconclusiva PCR Suspected PB cases Inconclusive histology Leprosy Diagnosis |
| title_short |
Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study |
| title_full |
Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study |
| title_fullStr |
Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study |
| title_full_unstemmed |
Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study |
| title_sort |
Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study |
| author |
Barbieri, Raquel R. |
| author_facet |
Barbieri, Raquel R. Manta, Fernanda S. N. Moreira, Suelen J. M. Sales, Anna M. Nery, José A. C. Nascimento, Lilian P. R. Hacker, Mariana A. Pacheco, Antonio G. Machado, Alice M. Sarno, Euzenir M. Moraes, Milton O. |
| author_role |
author |
| author2 |
Manta, Fernanda S. N. Moreira, Suelen J. M. Sales, Anna M. Nery, José A. C. Nascimento, Lilian P. R. Hacker, Mariana A. Pacheco, Antonio G. Machado, Alice M. Sarno, Euzenir M. Moraes, Milton O. |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Barbieri, Raquel R. Manta, Fernanda S. N. Moreira, Suelen J. M. Sales, Anna M. Nery, José A. C. Nascimento, Lilian P. R. Hacker, Mariana A. Pacheco, Antonio G. Machado, Alice M. Sarno, Euzenir M. Moraes, Milton O. |
| dc.subject.other.pt_BR.fl_str_mv |
Reação em Cadeia da Polimerase Hanseníase Diagnóstico Casos suspeitos Histologia inconclusiva |
| topic |
Reação em Cadeia da Polimerase Hanseníase Diagnóstico Casos suspeitos Histologia inconclusiva PCR Suspected PB cases Inconclusive histology Leprosy Diagnosis |
| dc.subject.en.pt_BR.fl_str_mv |
PCR Suspected PB cases Inconclusive histology Leprosy Diagnosis |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. |
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2019 |
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2019-09-10T15:33:58Z |
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2019-09-10T15:33:58Z |
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2019 |
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BARBIERI, Raquel R. et al. Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study. PLoS Neglected Tropical Diseases, v. 13, n. 3, p.1-12, Mar. 2019. |
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https://www.arca.fiocruz.br/handle/icict/35453 |
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1935-2727 |
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10.1371/journal.pntd.0007147 |
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1935-2735 |
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BARBIERI, Raquel R. et al. Quantitative polymerase chain reaction in paucibacillary leprosy diagnosis: A follow-up study. PLoS Neglected Tropical Diseases, v. 13, n. 3, p.1-12, Mar. 2019. 1935-2727 10.1371/journal.pntd.0007147 1935-2735 |
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