Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil

Detalhes bibliográficos
Autor(a) principal: Carreira, João Carlos Araujo
Data de Publicação: 2012
Outros Autores: Silva, Alba Valéria Machado da, Pereira, Daniela de Pita, Brazil, Reginaldo Peçanha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15401
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Núcleo Jacarepaguá. Rio de Janeiro, RJ, Brasil.
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spelling Carreira, João Carlos AraujoSilva, Alba Valéria Machado daPereira, Daniela de PitaBrazil, Reginaldo Peçanha2016-08-25T15:27:15Z2016-08-25T15:27:15Z2012CARREIRA, João Carlos Araujo; et al. Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil. Parasites & Vectors, v.5, n.111, 5p, 2012.1756-3305https://www.arca.fiocruz.br/handle/icict/1540110.1186/1756-3305-5-1111756-3305engBioMed CentralLeishmania infantumParasitismo tecidualAmastigotasDidelphis auritaLeishmania infantumTissular parasitismAmastigotesNatural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Núcleo Jacarepaguá. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica de Proteínas e Peptídeos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Molecular de Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil.BACKGROUND: The opossum Didelphis have been considered as natural hosts of Leishmania parasites in the New World, suggesting an important role in the epidemiology of Visceral Leishmaniasis (VL). Among six extant species that belong to the genus Didelphis, only two (D. marsupialis and D. albiventris), have been mentioned as natural hosts of Leishmania infantum in Brazil and Colombia. In the present paper, it is reported for the first time, the observation of intracellular parasites (amastigotes) in tissues of Didelphis aurita naturally infected with Leishmania infantum in Brazil. We also discuss some aspects associated to the relationship between L. infantum and the geographical distribution of some species of the genus Didelphis. METHODS: The opossums studied were caught by wire traps (Tomahawk) in Barra de Guaratiba, a peri-urban area in Rio de Janeiro. The opossums were killed with an overdose of Thiopental sodium.At necropsy, macroscopic alterations were examined and samples from liver, spleen, lymph nodes, ear, abdominal skin, scent glands and bone marrow were collected for parasitological and molecular diagnoses. RESULTS: Forty-eight opossums were captured in an AVL endemic region, 30 being caught in a mangrove area and eighteen animals in a forest area near to some residential-yards. Among the thirty opossums trapped in the mangrove area, all of them were negative by both imprint and sera samples assayed on Dipstick Tests, that is a test based on a combination of protein-A colloidal gold conjugate and rk39 Leishmania antigen to detect anti-Leishmania antibody in serum or plasma. At the macroscopic examination one out of eighteen opossums, caught close to the forest, presented alterations compatible with spleen hypertrophy and three were positive by Dipstick Tests (16.6%) and presented amastigotes in the spleen and in one of them, the parasites were also observed in a submandibular lymph node. Leishmania infantum infections were confirmed through dot blot hybridization using a L. infantum-specific biotinylated probe. CONCLUSIONS: In the present paper we present the first report of amastigotes in the tissues of Didelphis aurita (Mammalia: Marsupialia) naturally infected with Leishmania infantum. We also attempt to claim the particular role of some opossum species as hosts of Leishmania infantum, contributing at least in part on the description of potential sylvatic reservoirs.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15401/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALreginaldo_brazil_etal_IOC-2012.pdfreginaldo_brazil_etal_IOC-2012.pdfapplication/pdf456976https://www.arca.fiocruz.br/bitstream/icict/15401/2/reginaldo_brazil_etal_IOC-2012.pdfc5f5ba07135a7a5085af9ae1f9d31003MD52TEXTreginaldo_brazil_etal_IOC-2012.pdf.txtreginaldo_brazil_etal_IOC-2012.pdf.txtExtracted texttext/plain22859https://www.arca.fiocruz.br/bitstream/icict/15401/3/reginaldo_brazil_etal_IOC-2012.pdf.txt7b998d49db5e35eb59e39c4f7c7ba2d6MD53icict/154012018-04-02 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dc.title.pt_BR.fl_str_mv Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil
title Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil
spellingShingle Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil
Carreira, João Carlos Araujo
Leishmania infantum
Parasitismo tecidual
Amastigotas
Didelphis aurita
Leishmania infantum
Tissular parasitism
Amastigotes
title_short Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil
title_full Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil
title_fullStr Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil
title_full_unstemmed Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil
title_sort Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil
author Carreira, João Carlos Araujo
author_facet Carreira, João Carlos Araujo
Silva, Alba Valéria Machado da
Pereira, Daniela de Pita
Brazil, Reginaldo Peçanha
author_role author
author2 Silva, Alba Valéria Machado da
Pereira, Daniela de Pita
Brazil, Reginaldo Peçanha
author2_role author
author
author
dc.contributor.author.fl_str_mv Carreira, João Carlos Araujo
Silva, Alba Valéria Machado da
Pereira, Daniela de Pita
Brazil, Reginaldo Peçanha
dc.subject.other.pt_BR.fl_str_mv Leishmania infantum
Parasitismo tecidual
Amastigotas
topic Leishmania infantum
Parasitismo tecidual
Amastigotas
Didelphis aurita
Leishmania infantum
Tissular parasitism
Amastigotes
dc.subject.en.pt_BR.fl_str_mv Didelphis aurita
Leishmania infantum
Tissular parasitism
Amastigotes
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Núcleo Jacarepaguá. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2016-08-25T15:27:15Z
dc.date.available.fl_str_mv 2016-08-25T15:27:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARREIRA, João Carlos Araujo; et al. Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil. Parasites & Vectors, v.5, n.111, 5p, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15401
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/1756-3305-5-111
dc.identifier.eissn.none.fl_str_mv 1756-3305
identifier_str_mv CARREIRA, João Carlos Araujo; et al. Natural infection of Didelphis aurita (Mammalia: Marsupialia) with Leishmania infantum in Brazil. Parasites & Vectors, v.5, n.111, 5p, 2012.
1756-3305
10.1186/1756-3305-5-111
url https://www.arca.fiocruz.br/handle/icict/15401
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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