Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy

Detalhes bibliográficos
Autor(a) principal: Suss, Paula Hansen
Data de Publicação: 2015
Outros Autores: Capriglione, Luiz Guilherme, Barchiki, Fabiane, Miyague, Lye, Jackowski, Danielle, Fracaro, Letícia, Schittini, Andressa V., Senegaglia, Alexandra Cristina, Rebelatto, Carmen Lúcia Kuniyoshi, Olandoski, Marcia, Dominguez, Alejandro Correa, Brofman, Paulo Roberto Slud
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15682
Resumo: This work was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (CT-Saúde/MS/SCTIE/DECIT/MCT/CNPq N 17/2008) and Fundação Araucária (Convênio no. 416/2009).
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spelling Suss, Paula HansenCapriglione, Luiz GuilhermeBarchiki, FabianeMiyague, LyeJackowski, DanielleFracaro, LetíciaSchittini, Andressa V.Senegaglia, Alexandra CristinaRebelatto, Carmen Lúcia KuniyoshiOlandoski, MarciaDominguez, Alejandro CorreaBrofman, Paulo Roberto Slud2016-09-05T18:16:00Z2016-09-05T18:16:00Z2015SUSS, Paula Hansen et al. Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy. Experimental Biology and Medicine, v. 240, n. 7, p. 969-978, 2015.1535-3702https://www.arca.fiocruz.br/handle/icict/1568210.1177/1535370214565077This work was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (CT-Saúde/MS/SCTIE/DECIT/MCT/CNPq N 17/2008) and Fundação Araucária (Convênio no. 416/2009).Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.The development of new therapeutic strategies is necessary to reduce the worldwide social and economic impact of cardiovascular disease, which produces high rates of morbidity and mortality. A therapeutic option that has emerged in the last decade is cell therapy. The aim of this study was to compare the effect of transplanting human umbilical cord-derived stromal cells (UCSCs), human umbilical cord blood-derived endothelial cells (UCBECs) or a combination of these two cell types for the treatment of ischemic cardiomyopathy (IC) in a Wistar rat model. IC was induced by left coronary artery ligation, and baseline echocardiography was performed seven days later. Animals with a left ventricular ejection fraction (LVEF) of 40% were selected for the study. On the ninth day after IC was induced, the animals were randomized into the following experimental groups: UCSCs, UCBECs, UCSCs plus UCBECs, or vehicle (control). Thirty days after treatment, an echocardiographic analysis was performed, followed by euthanasia. The animals in all of the cell therapy groups, regardless of the cell type transplanted, had less collagen deposition in their heart tissue and demonstrated a significant improvement in myocardial function after IC. Furthermore, there was a trend of increasing numbers of blood vessels in the infarcted area. The median value of LVEF increased by 7.19% to 11.77%, whereas the control group decreased by 0.24%. These results suggest that UCSCs and UCBECs are promising cells for cellular cardiomyoplasty and can be an effective therapy for improving cardiac function following IC.porDirect intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleHuman umbilical cord-derived stromal cellsHuman umbilical cord blood-derived endothelial cellsCell therapyIschemic cardiomyopathyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15682/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSuss et al Direct intracardiac injection.pdfSuss et al Direct intracardiac 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dc.title.pt_BR.fl_str_mv Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy
title Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy
spellingShingle Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy
Suss, Paula Hansen
Human umbilical cord-derived stromal cells
Human umbilical cord blood-derived endothelial cells
Cell therapy
Ischemic cardiomyopathy
title_short Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy
title_full Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy
title_fullStr Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy
title_full_unstemmed Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy
title_sort Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy
author Suss, Paula Hansen
author_facet Suss, Paula Hansen
Capriglione, Luiz Guilherme
Barchiki, Fabiane
Miyague, Lye
Jackowski, Danielle
Fracaro, Letícia
Schittini, Andressa V.
Senegaglia, Alexandra Cristina
Rebelatto, Carmen Lúcia Kuniyoshi
Olandoski, Marcia
Dominguez, Alejandro Correa
Brofman, Paulo Roberto Slud
author_role author
author2 Capriglione, Luiz Guilherme
Barchiki, Fabiane
Miyague, Lye
Jackowski, Danielle
Fracaro, Letícia
Schittini, Andressa V.
Senegaglia, Alexandra Cristina
Rebelatto, Carmen Lúcia Kuniyoshi
Olandoski, Marcia
Dominguez, Alejandro Correa
Brofman, Paulo Roberto Slud
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Suss, Paula Hansen
Capriglione, Luiz Guilherme
Barchiki, Fabiane
Miyague, Lye
Jackowski, Danielle
Fracaro, Letícia
Schittini, Andressa V.
Senegaglia, Alexandra Cristina
Rebelatto, Carmen Lúcia Kuniyoshi
Olandoski, Marcia
Dominguez, Alejandro Correa
Brofman, Paulo Roberto Slud
dc.subject.en.pt_BR.fl_str_mv Human umbilical cord-derived stromal cells
Human umbilical cord blood-derived endothelial cells
Cell therapy
Ischemic cardiomyopathy
topic Human umbilical cord-derived stromal cells
Human umbilical cord blood-derived endothelial cells
Cell therapy
Ischemic cardiomyopathy
description This work was supported by grants from Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (CT-Saúde/MS/SCTIE/DECIT/MCT/CNPq N 17/2008) and Fundação Araucária (Convênio no. 416/2009).
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-09-05T18:16:00Z
dc.date.available.fl_str_mv 2016-09-05T18:16:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv SUSS, Paula Hansen et al. Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy. Experimental Biology and Medicine, v. 240, n. 7, p. 969-978, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15682
dc.identifier.issn.pt_BR.fl_str_mv 1535-3702
dc.identifier.doi.pt_BR.fl_str_mv 10.1177/1535370214565077
identifier_str_mv SUSS, Paula Hansen et al. Direct intracardiac injection of umbilical cord-derived stromal cells and umbilical cord blood-derived endothelial cells for the treatment of ischemic cardiomyopathy. Experimental Biology and Medicine, v. 240, n. 7, p. 969-978, 2015.
1535-3702
10.1177/1535370214565077
url https://www.arca.fiocruz.br/handle/icict/15682
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