Ultrastructural characterization of bovine umbilical cord blood cells

Detalhes bibliográficos
Autor(a) principal: Rodrigues,Gustavo C
Data de Publicação: 2010
Outros Autores: Oliveira,Lílian J, Monteiro,Janaína M, Lima,Ana R de, Gonçalez,Patricia O, Hernandez-Blazquez,Francisco J, Leiser,Rudolf, Kfoury Jr,José R
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010001000015
Resumo: The umbilical cord blood (UCB) is an important source of pluripotent stem cells, which motivated researches on ontogeny and transplantation. The morphological characterization of umbilical cord cells is the first step to establish subsequent experiments on these areas. Although some information on humans can be found, no data on UCB is available for bovines. Therefore, this work is the first attempt to conduct an ultrastructural characterization of bovine umbilical cord blood. Blood was collected from the umbilical cord of twenty fetuses by punction of the umbilical vein. Samples were processed for whole leucocytes observation by centrifugation and the buffy coat was collected. Cells were washed and pelleted and prepared according to the standard protocol of the transmission electron microscopy. The presence of cells with morphologic characteristics compatible with the precursors from the erythrocytic, neutrophilic, eosinophilic, basophilic, and lymphocytic lineages was observed. Atypical cells with peculiar morphological features, strongly similar to apoptotic cells, were seen. Bovine neutrophils with three types of cytoplasmic granules were also found in the blood. The ultrastructural characteristics of observed bovine UCB cells where similar to those found in other species, suggesting that bovines could possibly constitute an experimental model for approaches on UCB cells research.
id EMBRAPA-2_86996662eb035ea4951d0f6d9499ffc9
oai_identifier_str oai:scielo:S0100-736X2010001000015
network_acronym_str EMBRAPA-2
network_name_str Pesquisa Veterinária Brasileira (Online)
repository_id_str
spelling Ultrastructural characterization of bovine umbilical cord blood cellsUmbilical cord blood cellshaematopoietic progenitor cellsbovineultrastructureThe umbilical cord blood (UCB) is an important source of pluripotent stem cells, which motivated researches on ontogeny and transplantation. The morphological characterization of umbilical cord cells is the first step to establish subsequent experiments on these areas. Although some information on humans can be found, no data on UCB is available for bovines. Therefore, this work is the first attempt to conduct an ultrastructural characterization of bovine umbilical cord blood. Blood was collected from the umbilical cord of twenty fetuses by punction of the umbilical vein. Samples were processed for whole leucocytes observation by centrifugation and the buffy coat was collected. Cells were washed and pelleted and prepared according to the standard protocol of the transmission electron microscopy. The presence of cells with morphologic characteristics compatible with the precursors from the erythrocytic, neutrophilic, eosinophilic, basophilic, and lymphocytic lineages was observed. Atypical cells with peculiar morphological features, strongly similar to apoptotic cells, were seen. Bovine neutrophils with three types of cytoplasmic granules were also found in the blood. The ultrastructural characteristics of observed bovine UCB cells where similar to those found in other species, suggesting that bovines could possibly constitute an experimental model for approaches on UCB cells research.Colégio Brasileiro de Patologia Animal - CBPA2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010001000015Pesquisa Veterinária Brasileira v.30 n.10 2010reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2010001000015info:eu-repo/semantics/openAccessRodrigues,Gustavo COliveira,Lílian JMonteiro,Janaína MLima,Ana R deGonçalez,Patricia OHernandez-Blazquez,Francisco JLeiser,RudolfKfoury Jr,José Reng2010-12-06T00:00:00Zoai:scielo:S0100-736X2010001000015Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2010-12-06T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Ultrastructural characterization of bovine umbilical cord blood cells
title Ultrastructural characterization of bovine umbilical cord blood cells
spellingShingle Ultrastructural characterization of bovine umbilical cord blood cells
Rodrigues,Gustavo C
Umbilical cord blood cells
haematopoietic progenitor cells
bovine
ultrastructure
title_short Ultrastructural characterization of bovine umbilical cord blood cells
title_full Ultrastructural characterization of bovine umbilical cord blood cells
title_fullStr Ultrastructural characterization of bovine umbilical cord blood cells
title_full_unstemmed Ultrastructural characterization of bovine umbilical cord blood cells
title_sort Ultrastructural characterization of bovine umbilical cord blood cells
author Rodrigues,Gustavo C
author_facet Rodrigues,Gustavo C
Oliveira,Lílian J
Monteiro,Janaína M
Lima,Ana R de
Gonçalez,Patricia O
Hernandez-Blazquez,Francisco J
Leiser,Rudolf
Kfoury Jr,José R
author_role author
author2 Oliveira,Lílian J
Monteiro,Janaína M
Lima,Ana R de
Gonçalez,Patricia O
Hernandez-Blazquez,Francisco J
Leiser,Rudolf
Kfoury Jr,José R
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rodrigues,Gustavo C
Oliveira,Lílian J
Monteiro,Janaína M
Lima,Ana R de
Gonçalez,Patricia O
Hernandez-Blazquez,Francisco J
Leiser,Rudolf
Kfoury Jr,José R
dc.subject.por.fl_str_mv Umbilical cord blood cells
haematopoietic progenitor cells
bovine
ultrastructure
topic Umbilical cord blood cells
haematopoietic progenitor cells
bovine
ultrastructure
description The umbilical cord blood (UCB) is an important source of pluripotent stem cells, which motivated researches on ontogeny and transplantation. The morphological characterization of umbilical cord cells is the first step to establish subsequent experiments on these areas. Although some information on humans can be found, no data on UCB is available for bovines. Therefore, this work is the first attempt to conduct an ultrastructural characterization of bovine umbilical cord blood. Blood was collected from the umbilical cord of twenty fetuses by punction of the umbilical vein. Samples were processed for whole leucocytes observation by centrifugation and the buffy coat was collected. Cells were washed and pelleted and prepared according to the standard protocol of the transmission electron microscopy. The presence of cells with morphologic characteristics compatible with the precursors from the erythrocytic, neutrophilic, eosinophilic, basophilic, and lymphocytic lineages was observed. Atypical cells with peculiar morphological features, strongly similar to apoptotic cells, were seen. Bovine neutrophils with three types of cytoplasmic granules were also found in the blood. The ultrastructural characteristics of observed bovine UCB cells where similar to those found in other species, suggesting that bovines could possibly constitute an experimental model for approaches on UCB cells research.
publishDate 2010
dc.date.none.fl_str_mv 2010-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010001000015
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010001000015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0100-736X2010001000015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.30 n.10 2010
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
_version_ 1754122230509338624