Ultrastructural characterization of bovine umbilical cord blood cells
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010001000015 |
Resumo: | The umbilical cord blood (UCB) is an important source of pluripotent stem cells, which motivated researches on ontogeny and transplantation. The morphological characterization of umbilical cord cells is the first step to establish subsequent experiments on these areas. Although some information on humans can be found, no data on UCB is available for bovines. Therefore, this work is the first attempt to conduct an ultrastructural characterization of bovine umbilical cord blood. Blood was collected from the umbilical cord of twenty fetuses by punction of the umbilical vein. Samples were processed for whole leucocytes observation by centrifugation and the buffy coat was collected. Cells were washed and pelleted and prepared according to the standard protocol of the transmission electron microscopy. The presence of cells with morphologic characteristics compatible with the precursors from the erythrocytic, neutrophilic, eosinophilic, basophilic, and lymphocytic lineages was observed. Atypical cells with peculiar morphological features, strongly similar to apoptotic cells, were seen. Bovine neutrophils with three types of cytoplasmic granules were also found in the blood. The ultrastructural characteristics of observed bovine UCB cells where similar to those found in other species, suggesting that bovines could possibly constitute an experimental model for approaches on UCB cells research. |
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Ultrastructural characterization of bovine umbilical cord blood cellsUmbilical cord blood cellshaematopoietic progenitor cellsbovineultrastructureThe umbilical cord blood (UCB) is an important source of pluripotent stem cells, which motivated researches on ontogeny and transplantation. The morphological characterization of umbilical cord cells is the first step to establish subsequent experiments on these areas. Although some information on humans can be found, no data on UCB is available for bovines. Therefore, this work is the first attempt to conduct an ultrastructural characterization of bovine umbilical cord blood. Blood was collected from the umbilical cord of twenty fetuses by punction of the umbilical vein. Samples were processed for whole leucocytes observation by centrifugation and the buffy coat was collected. Cells were washed and pelleted and prepared according to the standard protocol of the transmission electron microscopy. The presence of cells with morphologic characteristics compatible with the precursors from the erythrocytic, neutrophilic, eosinophilic, basophilic, and lymphocytic lineages was observed. Atypical cells with peculiar morphological features, strongly similar to apoptotic cells, were seen. Bovine neutrophils with three types of cytoplasmic granules were also found in the blood. The ultrastructural characteristics of observed bovine UCB cells where similar to those found in other species, suggesting that bovines could possibly constitute an experimental model for approaches on UCB cells research.Colégio Brasileiro de Patologia Animal - CBPA2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010001000015Pesquisa Veterinária Brasileira v.30 n.10 2010reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/S0100-736X2010001000015info:eu-repo/semantics/openAccessRodrigues,Gustavo COliveira,Lílian JMonteiro,Janaína MLima,Ana R deGonçalez,Patricia OHernandez-Blazquez,Francisco JLeiser,RudolfKfoury Jr,José Reng2010-12-06T00:00:00Zoai:scielo:S0100-736X2010001000015Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2010-12-06T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Ultrastructural characterization of bovine umbilical cord blood cells |
title |
Ultrastructural characterization of bovine umbilical cord blood cells |
spellingShingle |
Ultrastructural characterization of bovine umbilical cord blood cells Rodrigues,Gustavo C Umbilical cord blood cells haematopoietic progenitor cells bovine ultrastructure |
title_short |
Ultrastructural characterization of bovine umbilical cord blood cells |
title_full |
Ultrastructural characterization of bovine umbilical cord blood cells |
title_fullStr |
Ultrastructural characterization of bovine umbilical cord blood cells |
title_full_unstemmed |
Ultrastructural characterization of bovine umbilical cord blood cells |
title_sort |
Ultrastructural characterization of bovine umbilical cord blood cells |
author |
Rodrigues,Gustavo C |
author_facet |
Rodrigues,Gustavo C Oliveira,Lílian J Monteiro,Janaína M Lima,Ana R de Gonçalez,Patricia O Hernandez-Blazquez,Francisco J Leiser,Rudolf Kfoury Jr,José R |
author_role |
author |
author2 |
Oliveira,Lílian J Monteiro,Janaína M Lima,Ana R de Gonçalez,Patricia O Hernandez-Blazquez,Francisco J Leiser,Rudolf Kfoury Jr,José R |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues,Gustavo C Oliveira,Lílian J Monteiro,Janaína M Lima,Ana R de Gonçalez,Patricia O Hernandez-Blazquez,Francisco J Leiser,Rudolf Kfoury Jr,José R |
dc.subject.por.fl_str_mv |
Umbilical cord blood cells haematopoietic progenitor cells bovine ultrastructure |
topic |
Umbilical cord blood cells haematopoietic progenitor cells bovine ultrastructure |
description |
The umbilical cord blood (UCB) is an important source of pluripotent stem cells, which motivated researches on ontogeny and transplantation. The morphological characterization of umbilical cord cells is the first step to establish subsequent experiments on these areas. Although some information on humans can be found, no data on UCB is available for bovines. Therefore, this work is the first attempt to conduct an ultrastructural characterization of bovine umbilical cord blood. Blood was collected from the umbilical cord of twenty fetuses by punction of the umbilical vein. Samples were processed for whole leucocytes observation by centrifugation and the buffy coat was collected. Cells were washed and pelleted and prepared according to the standard protocol of the transmission electron microscopy. The presence of cells with morphologic characteristics compatible with the precursors from the erythrocytic, neutrophilic, eosinophilic, basophilic, and lymphocytic lineages was observed. Atypical cells with peculiar morphological features, strongly similar to apoptotic cells, were seen. Bovine neutrophils with three types of cytoplasmic granules were also found in the blood. The ultrastructural characteristics of observed bovine UCB cells where similar to those found in other species, suggesting that bovines could possibly constitute an experimental model for approaches on UCB cells research. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010001000015 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2010001000015 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0100-736X2010001000015 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.30 n.10 2010 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122230509338624 |