Kdr genotyping in Aedes Aegypti from Brazil on a nation-wide scale from 2017 and 2018

Detalhes bibliográficos
Autor(a) principal: Costa, Monique Melo
Data de Publicação: 2020
Outros Autores: Campos, Kauara Brito, Brito, Luiz Paulo, Roux, Emmanuel, Rodovalho, Cynara Melo, Bellinato, Diogo Fernandes, Lima, José Bento Pereira, Martins, Ademir Jesus
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44943
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.
id CRUZ_5ca347b3addb621bb180592b9e22d016
oai_identifier_str oai:www.arca.fiocruz.br:icict/44943
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Costa, Monique MeloCampos, Kauara BritoBrito, Luiz PauloRoux, EmmanuelRodovalho, Cynara MeloBellinato, Diogo FernandesLima, José Bento PereiraMartins, Ademir Jesus2020-12-16T18:43:37Z2020-12-16T18:43:37Z2020COSTA, Monique Melo et al. Kdr genotyping in Aedes aegypti from Brazil on a nation-wide scale from 2017 to 2018. Sci Rep., v. 10, n. 1, 13267, 12p. Aug. 2020.2045-2322https://www.arca.fiocruz.br/handle/icict/4494310.1038/s41598-020-70029-7porNature ResearchAedes aegyptiGenotipagem de KdrEscala NacionalBrasil2017 a 2018Aedes aegyptiKdr genotypingNation-wide scalefrom 2017 to 2018BrazilKdr genotyping in Aedes Aegypti from Brazil on a nation-wide scale from 2017 and 2018info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.Universidade de Brasília. Faculdade de Medicina. Laboratório de Parasitologia Médica e Biologia de Vetores. Brasília, DF, Brasil / Ministério da Saúde. Secretaria de Vigilância em Saúde. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.ESPACE-DEV, IRD. Université de Montpellier. Université de La Réunion. Université de la Guyane and Université des Antilles, Montpellier, France / Fundação Oswaldo Cruz. aboratório Misto Internacional "Sentinela". Unb, IRD. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil / ESPACE-DEV, IRD. Université de Montpellier. Université de La Réunion. Université de la Guyane and Université des Antilles, Montpellier, France / Fundação Oswaldo Cruz. aboratório Misto Internacional "Sentinela". Unb, IRD. Rio de Janeiro, RJ, Brasil / Universidade federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Insecticide resistance is currently a threat to the control of Aedes agypti, the main vector of arboviruses in urban centers. Mutations in the voltage gated sodium channel (NaV), known as kdr (knockdown resistance), constitute an important selection mechanism for resistance against pyrethroids. In the present study, we investigated the kdr distribution for the Val1016Ile and Phe1534Cys alterations in Ae. aegypti from 123 Brazilian municipalities, based on SNP genotyping assays in over 5,500 mosquitoes. The alleles NaVS (1016Val+ + 1534Phe+), NaVR1 (1016Val+ + 1534Cyskdr) and NaVR2 (1016Ilekdr + 1534Cyskdr) were consistently observed, whereas kdr alleles have rapidly spread and increased in frequency. NaVS was the less frequent allele, mostly found in Northeastern populations. The highest allelic frequencies were observed for NaVR1, especially in the North, which was fixed in one Amazonian population. The double kdr NaVR2 was more prevalent in the Central-west and South-eastern populations. We introduce the 'kdr index', which revealed significant spatial patterns highlighting two to three distinct Brazilian regions. The 410L kdr mutation was additionally evaluated in 25 localities, evidencing that it generally occurs in the NaVR2 allele. This nationwide screening of a genetic mechanism for insecticide resistance is an important indication on how pyrethroid resistance in Ae. aegypti is evolving in Brazil.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44943/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAdemirJesus_Martins_etal_IOC_2020.pdfAdemirJesus_Martins_etal_IOC_2020.pdfapplication/pdf4296475https://www.arca.fiocruz.br/bitstream/icict/44943/2/AdemirJesus_Martins_etal_IOC_2020.pdf049fbc66edd925714efdc0e3e6e9a876MD52TEXTAdemirJesus_Martins_etal_IOC_2020.pdf.txtAdemirJesus_Martins_etal_IOC_2020.pdf.txtExtracted texttext/plain49160https://www.arca.fiocruz.br/bitstream/icict/44943/3/AdemirJesus_Martins_etal_IOC_2020.pdf.txt01b48406a03ed8e58aca7da62b3bfd14MD53icict/449432020-12-17 02:10:15.582oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352020-12-17T05:10:15Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Kdr genotyping in Aedes Aegypti from Brazil on a nation-wide scale from 2017 and 2018
title Kdr genotyping in Aedes Aegypti from Brazil on a nation-wide scale from 2017 and 2018
spellingShingle Kdr genotyping in Aedes Aegypti from Brazil on a nation-wide scale from 2017 and 2018
Costa, Monique Melo
Aedes aegypti
Genotipagem de Kdr
Escala Nacional
Brasil
2017 a 2018
Aedes aegypti
Kdr genotyping
Nation-wide scale
from 2017 to 2018
Brazil
title_short Kdr genotyping in Aedes Aegypti from Brazil on a nation-wide scale from 2017 and 2018
title_full Kdr genotyping in Aedes Aegypti from Brazil on a nation-wide scale from 2017 and 2018
title_fullStr Kdr genotyping in Aedes Aegypti from Brazil on a nation-wide scale from 2017 and 2018
title_full_unstemmed Kdr genotyping in Aedes Aegypti from Brazil on a nation-wide scale from 2017 and 2018
title_sort Kdr genotyping in Aedes Aegypti from Brazil on a nation-wide scale from 2017 and 2018
author Costa, Monique Melo
author_facet Costa, Monique Melo
Campos, Kauara Brito
Brito, Luiz Paulo
Roux, Emmanuel
Rodovalho, Cynara Melo
Bellinato, Diogo Fernandes
Lima, José Bento Pereira
Martins, Ademir Jesus
author_role author
author2 Campos, Kauara Brito
Brito, Luiz Paulo
Roux, Emmanuel
Rodovalho, Cynara Melo
Bellinato, Diogo Fernandes
Lima, José Bento Pereira
Martins, Ademir Jesus
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Monique Melo
Campos, Kauara Brito
Brito, Luiz Paulo
Roux, Emmanuel
Rodovalho, Cynara Melo
Bellinato, Diogo Fernandes
Lima, José Bento Pereira
Martins, Ademir Jesus
dc.subject.other.pt_BR.fl_str_mv Aedes aegypti
Genotipagem de Kdr
Escala Nacional
Brasil
2017 a 2018
topic Aedes aegypti
Genotipagem de Kdr
Escala Nacional
Brasil
2017 a 2018
Aedes aegypti
Kdr genotyping
Nation-wide scale
from 2017 to 2018
Brazil
dc.subject.en.pt_BR.fl_str_mv Aedes aegypti
Kdr genotyping
Nation-wide scale
from 2017 to 2018
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-12-16T18:43:37Z
dc.date.available.fl_str_mv 2020-12-16T18:43:37Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Monique Melo et al. Kdr genotyping in Aedes aegypti from Brazil on a nation-wide scale from 2017 to 2018. Sci Rep., v. 10, n. 1, 13267, 12p. Aug. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44943
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-020-70029-7
identifier_str_mv COSTA, Monique Melo et al. Kdr genotyping in Aedes aegypti from Brazil on a nation-wide scale from 2017 to 2018. Sci Rep., v. 10, n. 1, 13267, 12p. Aug. 2020.
2045-2322
10.1038/s41598-020-70029-7
url https://www.arca.fiocruz.br/handle/icict/44943
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/44943/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/44943/2/AdemirJesus_Martins_etal_IOC_2020.pdf
https://www.arca.fiocruz.br/bitstream/icict/44943/3/AdemirJesus_Martins_etal_IOC_2020.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
049fbc66edd925714efdc0e3e6e9a876
01b48406a03ed8e58aca7da62b3bfd14
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009167925903360

Registros relacionados