Identification of miRNAs enriched in extracellular vesicles derived from serum samples of Breast Cancer patients
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/40910 |
Resumo: | Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. |
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Ozawa, Patricia M. M.Vieira, EvelynLemos, Débora S.Souza, Ingrid L. MeloZanata, Silvio M.Pankievicz, Vânia C.Tuleski, Thalita R.Souza, Emanuel Maltempi deWowk, Pryscilla FaniniUrban, Cícero de AndradeKuroda, FlaviaLima, Rubens S.Almeida, Rodrigo C.Gradia, Daniela F.Cavalli, Iglenir JoãoCavalli, Luciane ReginaMalheiros, DanielleRibeiro, Enilze M. S. F.2020-04-21T18:15:49Z2020-04-21T18:15:49Z2020OZAWA, Patricia M. M. et al. Identification of miRNAs enriched in extracellular vesicles derived from serum samples of breast cancer patients. Biomolecules, v. 10, n. 150, p. 1-16, 2020.2218-273Xhttps://www.arca.fiocruz.br/handle/icict/4091010.3390/biom10010150porMDPIIdentification of miRNAs enriched in extracellular vesicles derived from serum samples of Breast Cancer patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Biologia Celular e Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Biologia Celular e Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Positivo. Faculdade de Medicina. Curitiba, PR, Brasil. / Hospital Nossa Senhora das Graças. Unidade de Mama. Curitiba, PR, Brasil.Hospital Nossa Senhora das Graças. Unidade de Mama. Curitiba, PR, Brasil.Hospital Nossa Senhora das Graças. Unidade de Mama. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. / Department of Biomedical Data Sciences. Molecular Epidemiology. Leiden University Medical Center. Leiden, The Netherlands.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Department of Oncology, Lombardi Comprehensive Cancer Center. Georgetown University. Washington, DC. USA. / Instituto de Pesquisa Pelé Pequeno Príncipe. Faculdades Pequeno Príncipe, Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil.MicroRNAs derived from extracellular vesicles (EV-miRNAs) are circulating miRNAs considered as potential new diagnostic markers for cancer that can be easily detected in liquid biopsies. In this study, we performed RNA sequencing analysis as a screening strategy to identify EV-miRNAs derived from serum of clinically well-annotated breast cancer (BC) patients from the south of Brazil. EVs from three groups of samples (healthy controls (CT), luminal A (LA), and triple-negative (TNBC)) were isolated from serum using a precipitation method and analyzed by RNA-seq (screening phase). Subsequently, four EV-miRNAs (miR-142-5p, miR-150-5p, miR-320a, and miR-4433b-5p) were selected to be quantified by quantitative real-time PCR (RT-qPCR) in individual samples (test phase). A panel composed of miR-142-5p, miR-320a, and miR-4433b-5p distinguished BC patients from CT with an area under the curve (AUC) of 0.8387 (93.33% sensitivity, 68.75% specificity). The combination of miR-142-5p and miR-320a distinguished LA patients from CT with an AUC of 0.9410 (100% sensitivity, 93.80% specificity). Interestingly, decreased expression of miR-142-5p and miR-150-5p were significantly associated with more advanced tumor grades (grade III), while the decreased expression of miR-142-5p and miR-320a was associated with a larger tumor size. These results provide insights into the potential application of EVs-miRNAs from serum as novel specific markers for early diagnosis of BC.Extracellular VesiclesBase SequenceLiquid BiopsyBreast NeoplasmsMicroARNsSecuencia de BasesBiopsia LíquidaNeoplasias de la MamaVesículas ExtracelularesMicroRNAsSequência de BasesBiópsia LíquidaNeoplasias da Mamainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/40910/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALbiomolecules-10.pdfbiomolecules-10.pdfapplication/pdf2485910https://www.arca.fiocruz.br/bitstream/icict/40910/2/biomolecules-10.pdf99405d2c15f065a8863674fe6cbf180cMD52TEXTbiomolecules-10.pdf.txtbiomolecules-10.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv |
Identification of miRNAs enriched in extracellular vesicles derived from serum samples of Breast Cancer patients |
title |
Identification of miRNAs enriched in extracellular vesicles derived from serum samples of Breast Cancer patients |
spellingShingle |
Identification of miRNAs enriched in extracellular vesicles derived from serum samples of Breast Cancer patients Ozawa, Patricia M. M. Extracellular Vesicles Base Sequence Liquid Biopsy Breast Neoplasms MicroARNs Secuencia de Bases Biopsia Líquida Neoplasias de la Mama Vesículas Extracelulares MicroRNAs Sequência de Bases Biópsia Líquida Neoplasias da Mama |
title_short |
Identification of miRNAs enriched in extracellular vesicles derived from serum samples of Breast Cancer patients |
title_full |
Identification of miRNAs enriched in extracellular vesicles derived from serum samples of Breast Cancer patients |
title_fullStr |
Identification of miRNAs enriched in extracellular vesicles derived from serum samples of Breast Cancer patients |
title_full_unstemmed |
Identification of miRNAs enriched in extracellular vesicles derived from serum samples of Breast Cancer patients |
title_sort |
Identification of miRNAs enriched in extracellular vesicles derived from serum samples of Breast Cancer patients |
author |
Ozawa, Patricia M. M. |
author_facet |
Ozawa, Patricia M. M. Vieira, Evelyn Lemos, Débora S. Souza, Ingrid L. Melo Zanata, Silvio M. Pankievicz, Vânia C. Tuleski, Thalita R. Souza, Emanuel Maltempi de Wowk, Pryscilla Fanini Urban, Cícero de Andrade Kuroda, Flavia Lima, Rubens S. Almeida, Rodrigo C. Gradia, Daniela F. Cavalli, Iglenir João Cavalli, Luciane Regina Malheiros, Danielle Ribeiro, Enilze M. S. F. |
author_role |
author |
author2 |
Vieira, Evelyn Lemos, Débora S. Souza, Ingrid L. Melo Zanata, Silvio M. Pankievicz, Vânia C. Tuleski, Thalita R. Souza, Emanuel Maltempi de Wowk, Pryscilla Fanini Urban, Cícero de Andrade Kuroda, Flavia Lima, Rubens S. Almeida, Rodrigo C. Gradia, Daniela F. Cavalli, Iglenir João Cavalli, Luciane Regina Malheiros, Danielle Ribeiro, Enilze M. S. F. |
author2_role |
author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ozawa, Patricia M. M. Vieira, Evelyn Lemos, Débora S. Souza, Ingrid L. Melo Zanata, Silvio M. Pankievicz, Vânia C. Tuleski, Thalita R. Souza, Emanuel Maltempi de Wowk, Pryscilla Fanini Urban, Cícero de Andrade Kuroda, Flavia Lima, Rubens S. Almeida, Rodrigo C. Gradia, Daniela F. Cavalli, Iglenir João Cavalli, Luciane Regina Malheiros, Danielle Ribeiro, Enilze M. S. F. |
dc.subject.en.pt_BR.fl_str_mv |
Extracellular Vesicles Base Sequence Liquid Biopsy Breast Neoplasms |
topic |
Extracellular Vesicles Base Sequence Liquid Biopsy Breast Neoplasms MicroARNs Secuencia de Bases Biopsia Líquida Neoplasias de la Mama Vesículas Extracelulares MicroRNAs Sequência de Bases Biópsia Líquida Neoplasias da Mama |
dc.subject.es.pt_BR.fl_str_mv |
MicroARNs Secuencia de Bases Biopsia Líquida Neoplasias de la Mama |
dc.subject.decs.pt_BR.fl_str_mv |
Vesículas Extracelulares MicroRNAs Sequência de Bases Biópsia Líquida Neoplasias da Mama |
description |
Universidade Federal do Paraná. Departamento de Genética. Curitiba, PR, Brasil. |
publishDate |
2020 |
dc.date.accessioned.fl_str_mv |
2020-04-21T18:15:49Z |
dc.date.available.fl_str_mv |
2020-04-21T18:15:49Z |
dc.date.issued.fl_str_mv |
2020 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OZAWA, Patricia M. M. et al. Identification of miRNAs enriched in extracellular vesicles derived from serum samples of breast cancer patients. Biomolecules, v. 10, n. 150, p. 1-16, 2020. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/40910 |
dc.identifier.issn.pt_BR.fl_str_mv |
2218-273X |
dc.identifier.doi.none.fl_str_mv |
10.3390/biom10010150 |
identifier_str_mv |
OZAWA, Patricia M. M. et al. Identification of miRNAs enriched in extracellular vesicles derived from serum samples of breast cancer patients. Biomolecules, v. 10, n. 150, p. 1-16, 2020. 2218-273X 10.3390/biom10010150 |
url |
https://www.arca.fiocruz.br/handle/icict/40910 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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MDPI |
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MDPI |
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