Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood

Detalhes bibliográficos
Autor(a) principal: Weinkopff, Tiffany
Data de Publicação: 2014
Outros Autores: Oliveira, Camila Indiani de, Carvalho, Augusto Marcelino Pedreira de, LaTorre, Yazmin Hauyon, Muniz, Aline de Couto, Miranda, José Carlos, Barral, Aldina Maria Prado, Cottier, Fabienne Tacchini
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8407
Resumo: University of Lausanne. Department of Biochemistry. WHO-Immunology Research and Training Center. Epalinges, Switzerland
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spelling Weinkopff, TiffanyOliveira, Camila Indiani deCarvalho, Augusto Marcelino Pedreira deLaTorre, Yazmin HauyonMuniz, Aline de CoutoMiranda, José CarlosBarral, Aldina Maria PradoCottier, Fabienne Tacchini2014-09-19T18:31:38Z2014-09-19T18:31:38Z2014WEINKOPFF, T. et al. Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood. PLoS Neglected Tropical Diseases, v. 8, n. 1, p. e2627, 2014.1935-273510.1371/journal.pntd.0002627https://www.arca.fiocruz.br/handle/icict/8407engPublic Library of ScienceRepeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human bloodinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Lausanne. Department of Biochemistry. WHO-Immunology Research and Training Center. Epalinges, SwitzerlandFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / National Institute of Science and Technology in Tropical Diseases. CNPq. Salvador, BA, BrasilUniversity of Lausanne. Department of Biochemistry. WHO-Immunology Research and Training Center. Epalinges, SwitzerlandUniversidade Federal da Bahia.Complexo Hospitalar Hospital Universitário Prof. Edgard Santos. Departamento de Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia (iii-INCT). Salvador, BA, BrasilUniversity of Lausanne. Department of Biochemistry. WHO-Immunology Research and Training Center. Epalinges, SwitzerlandDuring a blood meal, Lutzomyia intermedia sand flies transmit Leishmania braziliensis, a parasite causing tegumentary leishmaniasis. In experimental leishmaniasis, pre-exposure to saliva of most blood-feeding sand flies results in parasite establishment in absence of any skin damages in mice challenged with dermotropic Leishmania species together with saliva. In contrast, pre-immunization with Lu. intermedia salivary gland sonicate (SGS) results in enhanced skin inflammatory exacerbation upon co-inoculation of Lu. intermedia SGS and L. braziliensis. These data highlight potential unique features of both L. braziliensis and Lu. intermedia. In this study, we investigated the genes modulated by Lu. intermedia SGS immunization to understand their potential impact on the subsequent cutaneous immune response following inoculation of both SGS and L. braziliensis. The cellular recruitment and global gene expression profile was analyzed in mice repeatedly inoculated or not with Lu. intermedia. Microarray gene analysis revealed the upregulation of a distinct set of IFN-inducible genes, an immune signature not seen to the same extent in control animals. Of note this INF-inducible gene set was not induced in SGS pre-immunized mice subsequently co-inoculated with SGS and L. braziliensis. These data suggest the parasite prevented the upregulation of this Lu. intermedia saliva-related immune signature. The presence of these IFN-inducible genes was further analyzed in peripheral blood mononuclear cells (PBMCs) sampled from uninfected human individuals living in a L. braziliensis-endemic region of Brazil thus regularly exposed to Lu. intermedia bites. PBMCs were cultured in presence or absence of Lu. intermedia SGS. Using qRT-PCR we established that the IFN-inducible genes induced in the skin of SGS pre-immunized mice, were also upregulated by SGS in PBMCs from human individuals regularly exposed to Lu. intermedia bites, but not in PBMCs of control subjects. These data demonstrate that repeated exposure to Lu. intermedia SGS induces the expression of potentially host-protective IFN-inducible genes.Interações Hospedeiro-ParasitaLeishmania braziliensis/imunologiaPsychodidae/imunologiaSaliva/imunologiaAnimaisBrasilCélulas CultivadasFemininoPerfilação da Expressão GênicaHumanosInterferons/imunologiaLeucócitos Mononucleares/imunologiaCamundongosCamundongos Endogâmicos BALB CReação em Cadeia da Polimerase em Tempo Realinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8407/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALWeinkopff T Repeated....pdfWeinkopff T Repeated....pdfapplication/pdf983120https://www.arca.fiocruz.br/bitstream/icict/8407/2/Weinkopff%20T%20Repeated....pdfd59cdb8e924650004866148ab5a733ccMD52TEXTWeinkopff T Repeated....pdf.txtWeinkopff T Repeated....pdf.txtExtracted texttext/plain52353https://www.arca.fiocruz.br/bitstream/icict/8407/3/Weinkopff%20T%20Repeated....pdf.txt72bf8ac55224ae00e7cd9aa33c433907MD53icict/84072023-03-15 14:33:23.49oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood
title Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood
spellingShingle Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood
Weinkopff, Tiffany
Interações Hospedeiro-Parasita
Leishmania braziliensis/imunologia
Psychodidae/imunologia
Saliva/imunologia
Animais
Brasil
Células Cultivadas
Feminino
Perfilação da Expressão Gênica
Humanos
Interferons/imunologia
Leucócitos Mononucleares/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Reação em Cadeia da Polimerase em Tempo Real
title_short Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood
title_full Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood
title_fullStr Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood
title_full_unstemmed Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood
title_sort Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood
author Weinkopff, Tiffany
author_facet Weinkopff, Tiffany
Oliveira, Camila Indiani de
Carvalho, Augusto Marcelino Pedreira de
LaTorre, Yazmin Hauyon
Muniz, Aline de Couto
Miranda, José Carlos
Barral, Aldina Maria Prado
Cottier, Fabienne Tacchini
author_role author
author2 Oliveira, Camila Indiani de
Carvalho, Augusto Marcelino Pedreira de
LaTorre, Yazmin Hauyon
Muniz, Aline de Couto
Miranda, José Carlos
Barral, Aldina Maria Prado
Cottier, Fabienne Tacchini
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Weinkopff, Tiffany
Oliveira, Camila Indiani de
Carvalho, Augusto Marcelino Pedreira de
LaTorre, Yazmin Hauyon
Muniz, Aline de Couto
Miranda, José Carlos
Barral, Aldina Maria Prado
Cottier, Fabienne Tacchini
dc.subject.decs.pt_BR.fl_str_mv Interações Hospedeiro-Parasita
Leishmania braziliensis/imunologia
Psychodidae/imunologia
Saliva/imunologia
Animais
Brasil
Células Cultivadas
Feminino
Perfilação da Expressão Gênica
Humanos
Interferons/imunologia
Leucócitos Mononucleares/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Reação em Cadeia da Polimerase em Tempo Real
topic Interações Hospedeiro-Parasita
Leishmania braziliensis/imunologia
Psychodidae/imunologia
Saliva/imunologia
Animais
Brasil
Células Cultivadas
Feminino
Perfilação da Expressão Gênica
Humanos
Interferons/imunologia
Leucócitos Mononucleares/imunologia
Camundongos
Camundongos Endogâmicos BALB C
Reação em Cadeia da Polimerase em Tempo Real
description University of Lausanne. Department of Biochemistry. WHO-Immunology Research and Training Center. Epalinges, Switzerland
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-09-19T18:31:38Z
dc.date.available.fl_str_mv 2014-09-19T18:31:38Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv WEINKOPFF, T. et al. Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood. PLoS Neglected Tropical Diseases, v. 8, n. 1, p. e2627, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8407
dc.identifier.issn.none.fl_str_mv 1935-2735
10.1371/journal.pntd.0002627
identifier_str_mv WEINKOPFF, T. et al. Repeated exposure to Lutzomyia intermedia sand fly saliva induces local expression of interferon-inducible genes both at the site of injection in mice and in human blood. PLoS Neglected Tropical Diseases, v. 8, n. 1, p. e2627, 2014.
1935-2735
10.1371/journal.pntd.0002627
url https://www.arca.fiocruz.br/handle/icict/8407
dc.language.iso.fl_str_mv eng
language eng
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dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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