Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria

Detalhes bibliográficos
Autor(a) principal: Rangel, Karyne
Data de Publicação: 2022
Outros Autores: Cabral, Fellipe O., Lechuga, Guilherme C., Carvalho, João P. R. S., Villas-Bôas, Maria H. S., Midlej, Victor, De-Simone, Salvatore G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/54979
Resumo: Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular (LESM). Rio de Janeiro, RJ, Brasil.
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spelling Rangel, KaryneCabral, Fellipe O.Lechuga, Guilherme C.Carvalho, João P. R. S.Villas-Bôas, Maria H. S.Midlej, VictorDe-Simone, Salvatore G.2022-10-04T13:26:48Z2022-10-04T13:26:48Z2022RANGEL, Karine et al. Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria. Molecules, v. 27, 3998, p. 1 - 17, June 2022.1420-3049https://www.arca.fiocruz.br/handle/icict/5497910.3390/ molecules27133998engMDPIOzônioBactéria patogênicaResistência antimicrobianaSEMPatógenos ESKAPEActividade antimicrobianaOzonePathogenic bacteriaAntimicrobial resistanceSEMESKAPE pathogensAntimicrobial activityPotent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular (LESM). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular (LESM). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular (LESM). Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Departamento de Biologia Molecular e Celular. Programa de Pós-Graduação em Ciências e Biotecnologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Celular e Ultraestrutura. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular (LESM). Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Departamento de Biologia Celular e Molecular. Programa de Pós Graduação em Ciência e Biotecnologia. Niterói, RJ, Brasil.Background: Health care-associated infections (HAIs) are a significant public health problem worldwide, favoring multidrug-resistant (MDR) microorganisms. The SARS-CoV-2 infection was negatively associated with the increase in antimicrobial resistance, and the ESKAPE group had the most significant impact on HAIs. The study evaluated the bactericidal effect of a high concentration of O3 gas on some reference and ESKAPE bacteria. Material and Methods: Four standard strains and four clinical or environmental MDR strains were exposed to elevated ozone doses at different concentrations and times. Bacterial inactivation (growth and cultivability) was investigated using colony counts and resazurin as metabolic indicators. Scanning electron microscopy (SEM) was performed. Results: The culture exposure to a high level of O3 inhibited the growth of all bacterial strains tested with a statistically significant reduction in colony count compared to the control group. The cell viability of S. aureus (MRSA) (99.6%) and P. aeruginosa (XDR) (29.2%) was reduced considerably, and SEM showed damage to bacteria after O3 treatment Conclusion: The impact of HAIs can be easily dampened by the widespread use of ozone in ICUs. This product usually degrades into molecular oxygen and has a low toxicity compared to other sanitization products. However, high doses of ozone were able to interfere with the growth of all strains studied, evidencing that ozone-based decontamination approaches may represent the future of hospital cleaning methods.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/54979/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALKaryneRangel_SalvatoreDeSimone_etal_IOC_2022.pdfKaryneRangel_SalvatoreDeSimone_etal_IOC_2022.pdfapplication/pdf2765519https://www.arca.fiocruz.br/bitstream/icict/54979/2/KaryneRangel_SalvatoreDeSimone_etal_IOC_2022.pdf480dc4501ac8e4205485e2e40a31e5baMD52icict/549792023-09-04 11:10:24.518oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:10:24Repositório Institucional da FIOCRUZ (ARCA) - 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dc.title.en_US.fl_str_mv Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria
title Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria
spellingShingle Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria
Rangel, Karyne
Ozônio
Bactéria patogênica
Resistência antimicrobiana
SEM
Patógenos ESKAPE
Actividade antimicrobiana
Ozone
Pathogenic bacteria
Antimicrobial resistance
SEM
ESKAPE pathogens
Antimicrobial activity
title_short Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria
title_full Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria
title_fullStr Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria
title_full_unstemmed Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria
title_sort Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria
author Rangel, Karyne
author_facet Rangel, Karyne
Cabral, Fellipe O.
Lechuga, Guilherme C.
Carvalho, João P. R. S.
Villas-Bôas, Maria H. S.
Midlej, Victor
De-Simone, Salvatore G.
author_role author
author2 Cabral, Fellipe O.
Lechuga, Guilherme C.
Carvalho, João P. R. S.
Villas-Bôas, Maria H. S.
Midlej, Victor
De-Simone, Salvatore G.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rangel, Karyne
Cabral, Fellipe O.
Lechuga, Guilherme C.
Carvalho, João P. R. S.
Villas-Bôas, Maria H. S.
Midlej, Victor
De-Simone, Salvatore G.
dc.subject.other.en_US.fl_str_mv Ozônio
Bactéria patogênica
Resistência antimicrobiana
SEM
Patógenos ESKAPE
Actividade antimicrobiana
topic Ozônio
Bactéria patogênica
Resistência antimicrobiana
SEM
Patógenos ESKAPE
Actividade antimicrobiana
Ozone
Pathogenic bacteria
Antimicrobial resistance
SEM
ESKAPE pathogens
Antimicrobial activity
dc.subject.en.en_US.fl_str_mv Ozone
Pathogenic bacteria
Antimicrobial resistance
SEM
ESKAPE pathogens
Antimicrobial activity
description Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Instituto Nacional de Ciência e Tecnologia para Inovação em Doenças da População Negligenciadas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular (LESM). Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-10-04T13:26:48Z
dc.date.available.fl_str_mv 2022-10-04T13:26:48Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RANGEL, Karine et al. Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria. Molecules, v. 27, 3998, p. 1 - 17, June 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/54979
dc.identifier.issn.en_US.fl_str_mv 1420-3049
dc.identifier.doi.none.fl_str_mv 10.3390/ molecules27133998
identifier_str_mv RANGEL, Karine et al. Potent Activity of a High Concentration of Chemical Ozone against Antibiotic-Resistant Bacteria. Molecules, v. 27, 3998, p. 1 - 17, June 2022.
1420-3049
10.3390/ molecules27133998
url https://www.arca.fiocruz.br/handle/icict/54979
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/54979/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/54979/2/KaryneRangel_SalvatoreDeSimone_etal_IOC_2022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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