Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom

Detalhes bibliográficos
Autor(a) principal: Resende, L.M.
Data de Publicação: 2017
Outros Autores: Almeida, J.R., Ramos, R. Schezaro, Collaço, R.C.O., Simioni, L.R., Ramírez, D., González, W., Soares, Andreimar Martins, Calderon, Leonardo Azevedo, Marangoni, S., Silva, S.L. da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/47354
Resumo: Campinas State University. Institute of Biology. Department of Biochemistry and Tissue Biology. Campinas, SP, Brasil.
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spelling Resende, L.M.Almeida, J.R.Ramos, R. SchezaroCollaço, R.C.O.Simioni, L.R.Ramírez, D.González, W.Soares, Andreimar MartinsCalderon, Leonardo AzevedoMarangoni, S.Silva, S.L. da2021-05-25T13:57:18Z2021-05-25T13:57:18Z2017L.M. Resende, et al. Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom, Toxicon, Volume 127, Pages 22-36, 2017.0041-0101https://www.arca.fiocruz.br/handle/icict/473541879-3150engElsevierExploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venominfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCampinas State University. Institute of Biology. Department of Biochemistry and Tissue Biology. Campinas, SP, Brasil.Universidad Regional Amazônica. Tena, Napo, Ecuador. / International Network of Ecuadorian Snakes Venom Studies. Ecuador.State University of Campinas. Faculty of Medical Sciences. Department of Pharmacology. Campinas, SP, Brasil.State University of Campinas. Faculty of Medical Sciences. Department of Pharmacology. Campinas, SP, Brasil.State University of Campinas. Faculty of Medical Sciences. Department of Pharmacology. Campinas, SP, Brasil.Universidad de Talca. Centro de Bioinformática y Simulación Molecular. Talca, Chile.Universidad de Talca. Centro de Bioinformática y Simulación Molecular. Talca, Chile. / International Network of Ecuadorian Snakes Venom Studies. Ecuador.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas. Federal University of Rondônia. Porto Velho, RO, Brasil. / International Network of Ecuadorian Snakes Venom Studies. Ecuador.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas. Federal University of Rondônia. Porto Velho, RO, Brasil. / International Network of Ecuadorian Snakes Venom Studies. Ecuador.Campinas State University. Institute of Biology. Department of Biochemistry and Tissue Biology. Campinas, SP, Brasil.Universidad Regional Amazônica. Tena, Napo, Ecuador. / International Network of Ecuadorian Snakes Venom Studies. Ecuador.Phospholipases A2 (PLA2s) constitute a class of extensively studied toxins, isolated from snake venoms. Basic PLA2 isoforms mediate various toxicological effects, while the acidic isoforms generally have higher enzymatic activities, but do not promote evident toxic effects. The functions of these acidic isoforms in snake venoms are still not completely understood and more studies are needed to characterize the biological functions and diversification of acidic toxins in order to justify their abundant presence in these secretions. Recently, Lomonte and collaborators demonstrated, in a proteomic and toxicological study, high concentrations of PLA2s in the venom of Agkistrodon piscivorus leucostoma. We have, herein, purified and characterized an acidic PLA2 from this snake venom, denominated AplTx-I, in order to better understand its biochemical and structural characteristics, as well as its biological effects. AplTx-I was purified using two chromatographic steps, in association with enzymatic and biological assays. The acidic toxin was found to be one of the most abundant proteins in the venom of A. p. leucostoma; the protein was monomeric with a molecular mass of 13,885.8 Da, as identified by mass spectrometry ESI-TOF and electrophoresis. The toxin has similar primary and tridimensional structures to those of other acidic PLA2s, a theoretical and experimental isoelectric point of z5.12, and a calcium-dependent enzyme activity of 25.8985 nM/min/mg, with maximum values at 37 C and pH 8.0. Despite its high enzymatic activity on synthetic substrate, AplTx-I did not induce high or significant myotoxic, coagulant, anticoagulant, edema, neuromuscular toxicity in mouse phrenic nerve-diaphragm preparations or antibacterial activities. Interestingly, AplTx-I triggered a high and selective neuromuscular toxicity in chick biventer cervicis preparations. These findings are relevant to provide a deeper understanding of the pharmacology, role and diversification of acidic phospholipase A2 isoforms in snake venoms.Acidic phospholipase A2Snake venomAgkistrodon piscivorus leucostomaPharmacological activityinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/47354/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALExploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake.pdfExploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake.pdfapplication/pdf3455762https://www.arca.fiocruz.br/bitstream/icict/47354/2/Exploring%20and%20understanding%20the%20functional%20role%2c%20and%20biochemical%20and%20structural%20characteristics%20of%20an%20acidic%20phospholipase%20A2%2c%20AplTx-I%2c%20purified%20from%20Agkistrodon%20piscivorus%20leucostoma%20snake.pdf2f888b714f1081fa85342f86d26e996eMD52TEXTExploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake.pdf.txtExploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom
title Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom
spellingShingle Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom
Resende, L.M.
Acidic phospholipase A2
Snake venom
Agkistrodon piscivorus leucostoma
Pharmacological activity
title_short Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom
title_full Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom
title_fullStr Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom
title_full_unstemmed Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom
title_sort Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom
author Resende, L.M.
author_facet Resende, L.M.
Almeida, J.R.
Ramos, R. Schezaro
Collaço, R.C.O.
Simioni, L.R.
Ramírez, D.
González, W.
Soares, Andreimar Martins
Calderon, Leonardo Azevedo
Marangoni, S.
Silva, S.L. da
author_role author
author2 Almeida, J.R.
Ramos, R. Schezaro
Collaço, R.C.O.
Simioni, L.R.
Ramírez, D.
González, W.
Soares, Andreimar Martins
Calderon, Leonardo Azevedo
Marangoni, S.
Silva, S.L. da
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Resende, L.M.
Almeida, J.R.
Ramos, R. Schezaro
Collaço, R.C.O.
Simioni, L.R.
Ramírez, D.
González, W.
Soares, Andreimar Martins
Calderon, Leonardo Azevedo
Marangoni, S.
Silva, S.L. da
dc.subject.en.pt_BR.fl_str_mv Acidic phospholipase A2
Snake venom
Agkistrodon piscivorus leucostoma
Pharmacological activity
topic Acidic phospholipase A2
Snake venom
Agkistrodon piscivorus leucostoma
Pharmacological activity
description Campinas State University. Institute of Biology. Department of Biochemistry and Tissue Biology. Campinas, SP, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2021-05-25T13:57:18Z
dc.date.available.fl_str_mv 2021-05-25T13:57:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv L.M. Resende, et al. Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom, Toxicon, Volume 127, Pages 22-36, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/47354
dc.identifier.issn.pt_BR.fl_str_mv 0041-0101
dc.identifier.eissn.none.fl_str_mv 1879-3150
identifier_str_mv L.M. Resende, et al. Exploring and understanding the functional role, and biochemical and structural characteristics of an acidic phospholipase A2, AplTx-I, purified from Agkistrodon piscivorus leucostoma snake venom, Toxicon, Volume 127, Pages 22-36, 2017.
0041-0101
1879-3150
url https://www.arca.fiocruz.br/handle/icict/47354
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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