Cytokines, chemokines and leukotrienes profile and signature analysis in HTLV-1 infection as an evidence of disease progression

• Autor(a) principal: Starling, Ana Lúcia Borges
• Data de Publicação: 2014
• Outros Autores: Gonçalves, Denise Utsch, Magalhães, Vanessa Peruhype, Reis, Jordana Coelho dos, Lambertucci, José Roberto, Labanca, Ludimila, Pereira, Silvio Roberto Souza, Martins, Marina L, Ribas, João G, Carvalho, Andréa Teixeira de, Trindade, Bruno Caetano, Faccioli, Lúcia Helena, Proietti, Anna Bárbara de Freitas Carneiro, Martins Filho, Olindo Assis
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
• Texto Completo: https://www.arca.fiocruz.br/handle/icict/50909

Background: A cross-sectional study evaluated the cytokines, chemokines and leukotrienes profiles as possible biomarkers of progression to HTLV-1 associated myelopathy (HAM). Methods: Serum samples from 21 healthy blood donors (HBD), 27 asymptomatic carriers (ASC), 32 possible HAM (pHAM) and 28 HAM individuals were tested for cytokines (IL-6, IFN-γ, TNF-α, IL-2, IL-4 and IL-10), chemokines ( RANTES, MCP1, IL-8, MIG and IP-10) and leukotrienes (CysLTs and LTB-4). For each molecule tested, the HTLV-1 individuals were classified as low or high-producers taking the global median index of the HBD group as a cut off. Results: When comparing AS and pHAM individuals, AS were high-producers of IP-10 and low-producers of RANTES; pHAM were high-producers of IL-2 and low of IL-8. Besides, AS individuals presented a strong positive correlation between the regulatory cytokines IL-10 with IL-4 and between both with the pro-inflammatory cytokines IL-2 and IL-6; a negative correlation was found between RANTES and IL-2. HAM were high-producers of IL-6, IFN-γ, IP-10, LTB4, IL-4, MIG, IL-10, IL-2, presented a positive correlation of TNF-α and IFN-γ with IL-6, but this group had a positive correlation of CysLT with IL-10, IL-4 and TNF-α, contrasting with other groups. Discussion: HAM displayed a unique signature of inflammation, which was strengthened by CysLT and not counterbalanced by IL-4 and IL-10. This signature was observed in pHAM to a lower extent, becoming more evident in HAM. This profile may indicate disease progression and may serve as prognostic markers in future studies.