A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom

Detalhes bibliográficos
Autor(a) principal: Trevisan-Silva, Dilza
Data de Publicação: 2017
Outros Autores: Bednaski, Aline V., Fischer, Juliana de Saldanha da Gama, Veiga, Silvio S., Bandeira, Nuno, Guthals, Adrian, Marchini, Fabrício Klerynton, Leprevost, Felipe da Veiga, Barbosa, Valmir Carneiro, Senff-Ribeiro, Andrea, Carvalho, Paulo Costa
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/20935
Resumo: Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil.
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spelling Trevisan-Silva, DilzaBednaski, Aline V.Fischer, Juliana de Saldanha da GamaVeiga, Silvio S.Bandeira, NunoGuthals, AdrianMarchini, Fabrício KleryntonLeprevost, Felipe da VeigaBarbosa, Valmir CarneiroSenff-Ribeiro, AndreaCarvalho, Paulo Costa2017-09-06T18:49:14Z2017-09-06T18:49:14Z2017TREVISAN-SILVA, Dilza et al. Data Descriptor: A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom. Scientific Data, v. 4, p.1-7, 2017.2052-4463https://www.arca.fiocruz.br/handle/icict/2093510.1038/sdata.2017.90porNature Publishing GroupAranha Marrom ReclusaBrown Recluse SpiderSpider VenomsSequence AnalysisAraña Reclusa PardaVenenos de ArañaAnálisis de Secuencia de ProteínaVenenos de AranhaAnálise de Sequência de ProteínaA multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venominfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Grupo de Proteômica e Espectrometria de Massas Computacional. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil.Center for Computational Mass Spectrometry. University of California. San Diego, USA.Center for Computational Mass Spectrometry. University of California. San Diego, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Plataforma de Espectrometria de Massas RPT02H. Curitiba, PR, BrasilFundação Oswaldo Cruz. Instituto Carlos Chagas. Grupo de Proteômica e Espectrometria de Massas Computacional. Curitiba, PR, Brasil.Federal University of Rio de Janeiro. COPPE. Systems Engineering and Computer Science Program. Rio de Janeiro, RJ, Brasil.Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Grupo de Proteômica e Espectrometria de Massas Computacional. Curitiba, PR, Brasil.Venoms are a rich source for the discovery of molecules with biotechnological applications, but their analysis is challenging even for state-of-the-art proteomics. Here we report on a large-scale proteomic assessment of the venom of Loxosceles intermedia, the so-called brown spider. Venom was extracted from 200 spiders and fractioned into two aliquots relative to a 10 kDa cutoff mass. Each of these was further fractioned and digested with trypsin (4 h), trypsin (18 h), pepsin (18 h), and chymotrypsin (18 h), then analyzed by MudPIT on an LTQ-Orbitrap XL ETD mass spectrometer fragmenting precursors by CID, HCD, and ETD. Aliquots of undigested samples were also analyzed. Our experimental design allowed us to apply spectral networks, thus enabling us to obtain meta-contig assemblies, and consequently de novo sequencing of practically complete proteins, culminating in a deep proteome assessment of the venom. Data are available via ProteomeXchange, with identifier PXD005523.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/20935/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALTrevisan-Mproteas.pdfTrevisan-Mproteas.pdfapplication/pdf349753https://www.arca.fiocruz.br/bitstream/icict/20935/2/Trevisan-Mproteas.pdfa53487aa6a3bb4e7e558a4867496c7a1MD52TEXTTrevisan-Mproteas.pdf.txtTrevisan-Mproteas.pdf.txtExtracted texttext/plain38173https://www.arca.fiocruz.br/bitstream/icict/20935/3/Trevisan-Mproteas.pdf.txt443474136a31c5194f89a8f792fdc30fMD53icict/209352019-12-15 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dc.title.pt_BR.fl_str_mv A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom
title A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom
spellingShingle A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom
Trevisan-Silva, Dilza
Aranha Marrom Reclusa
Brown Recluse Spider
Spider Venoms
Sequence Analysis
Araña Reclusa Parda
Venenos de Araña
Análisis de Secuencia de Proteína
Venenos de Aranha
Análise de Sequência de Proteína
title_short A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom
title_full A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom
title_fullStr A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom
title_full_unstemmed A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom
title_sort A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom
author Trevisan-Silva, Dilza
author_facet Trevisan-Silva, Dilza
Bednaski, Aline V.
Fischer, Juliana de Saldanha da Gama
Veiga, Silvio S.
Bandeira, Nuno
Guthals, Adrian
Marchini, Fabrício Klerynton
Leprevost, Felipe da Veiga
Barbosa, Valmir Carneiro
Senff-Ribeiro, Andrea
Carvalho, Paulo Costa
author_role author
author2 Bednaski, Aline V.
Fischer, Juliana de Saldanha da Gama
Veiga, Silvio S.
Bandeira, Nuno
Guthals, Adrian
Marchini, Fabrício Klerynton
Leprevost, Felipe da Veiga
Barbosa, Valmir Carneiro
Senff-Ribeiro, Andrea
Carvalho, Paulo Costa
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Trevisan-Silva, Dilza
Bednaski, Aline V.
Fischer, Juliana de Saldanha da Gama
Veiga, Silvio S.
Bandeira, Nuno
Guthals, Adrian
Marchini, Fabrício Klerynton
Leprevost, Felipe da Veiga
Barbosa, Valmir Carneiro
Senff-Ribeiro, Andrea
Carvalho, Paulo Costa
dc.subject.other.pt_BR.fl_str_mv Aranha Marrom Reclusa
topic Aranha Marrom Reclusa
Brown Recluse Spider
Spider Venoms
Sequence Analysis
Araña Reclusa Parda
Venenos de Araña
Análisis de Secuencia de Proteína
Venenos de Aranha
Análise de Sequência de Proteína
dc.subject.en.pt_BR.fl_str_mv Brown Recluse Spider
Spider Venoms
Sequence Analysis
dc.subject.es.pt_BR.fl_str_mv Araña Reclusa Parda
Venenos de Araña
Análisis de Secuencia de Proteína
dc.subject.decs.pt_BR.fl_str_mv Venenos de Aranha
Análise de Sequência de Proteína
description Universidade Federal do Paraná. Departamento de Biologia Celular. Curitiba, PR, Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-09-06T18:49:14Z
dc.date.available.fl_str_mv 2017-09-06T18:49:14Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TREVISAN-SILVA, Dilza et al. Data Descriptor: A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom. Scientific Data, v. 4, p.1-7, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/20935
dc.identifier.issn.pt_BR.fl_str_mv 2052-4463
dc.identifier.doi.none.fl_str_mv 10.1038/sdata.2017.90
identifier_str_mv TREVISAN-SILVA, Dilza et al. Data Descriptor: A multi-protease, multi-dissociation, bottom-up-to-top-down proteomic view of the Loxosceles intermedia venom. Scientific Data, v. 4, p.1-7, 2017.
2052-4463
10.1038/sdata.2017.90
url https://www.arca.fiocruz.br/handle/icict/20935
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/20935/2/Trevisan-Mproteas.pdf
https://www.arca.fiocruz.br/bitstream/icict/20935/3/Trevisan-Mproteas.pdf.txt
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