A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism

Detalhes bibliográficos
Autor(a) principal: Alvarenga, Larissa Magalhães
Data de Publicação: 2021
Outros Autores: Cardenas, Guillermo Andrés Cerquera, Jiacomini, Isabella Gizzi, Ramírez, Marcel Ivan
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/49741
Resumo: Universidade Federal do Paraná. Departamento de Patologia Básica. Laboratório de Imunoquímica. Curitiba, PR, Brasil.
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spelling Alvarenga, Larissa MagalhãesCardenas, Guillermo Andrés CerqueraJiacomini, Isabella GizziRamírez, Marcel Ivan2021-11-09T12:46:37Z2021-11-09T12:46:37Z2021ALVARENGA, Larissa Magalhães et al. A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism. Toxicology Letters, n. 350, p. 202–212, 2021.0378-4274https://www.arca.fiocruz.br/handle/icict/4974110.1016/j.toxlet.2021.07.014porElsevierA new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelisminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Departamento de Patologia Básica. Laboratório de Imunoquímica. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Patologia Básica. Laboratório de Imunoquímica. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Patologia Básica. Laboratório de Imunoquímica. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. EVAHPI – Extracellular Vesicles and Host-Parasite Interactions Research Group. Curitiba, PR, Brasil.Envenomation by the Loxosceles genus spiders is a recurring health issue worldwide and specially in the Americas. The physiopathology of the envenomation is tightly associated to the venom’s rich toxin composition, able to produce a local dermonecrotic lesion that can evolve systemically and if worsened, might result in multiple organ failure and lethality. The cellular and molecular mechanisms involved with the physiopathology of Loxoscelism are not completely understood, however, the venom’s Phospholipases D (PLDs) are known to trigger membrane injury in various cell types. Here, we report for the first time the Loxosceles venom’s ability to stimulate the production of extracellular vesicles (EVs) in various human cell lineages. Components of the Loxosceles venom were also detectable in the cargo of these vesicles, suggesting that they may be implicated in the process of extracellular venom release. EVs from venom treated cells exhibited phospholipase D activity and were able to induce in vitro hemolysis in human red blood cells and alter the HEK cell membranes’ permeability. Nonetheless, the PLD activity was inhibited when an anti-venom PLDs monoclonal antibody was co-administered with the whole venom. In summary, our findings shed new light on the mechanisms underlying cellular events in the context of loxoscelism and suggest a crucial role of EVs in the process of envenomation.LoxoscelesBrown Recluse SpiderPhospholipases DGroup II Phospholipases A2Extracellular VesiclesEnvenomationAraña Reclusa PardaFosfolipasas A2 Grupo IIVesículas ExtracelularesEnvenenamientoAraignée recluse bruneGroup II Phospholipases A2Vésicules extracellulairesempoisonnementAranha Marrom ReclusaFosfolipases A2 do Grupo IIVesículas ExtracelularesEnvenenamentoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism
title A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism
spellingShingle A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism
Alvarenga, Larissa Magalhães
Loxosceles
Brown Recluse Spider
Phospholipases D
Group II Phospholipases A2
Extracellular Vesicles
Envenomation
Araña Reclusa Parda
Fosfolipasas A2 Grupo II
Vesículas Extracelulares
Envenenamiento
Araignée recluse brune
Group II Phospholipases A2
Vésicules extracellulaires
empoisonnement
Aranha Marrom Reclusa
Fosfolipases A2 do Grupo II
Vesículas Extracelulares
Envenenamento
title_short A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism
title_full A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism
title_fullStr A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism
title_full_unstemmed A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism
title_sort A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism
author Alvarenga, Larissa Magalhães
author_facet Alvarenga, Larissa Magalhães
Cardenas, Guillermo Andrés Cerquera
Jiacomini, Isabella Gizzi
Ramírez, Marcel Ivan
author_role author
author2 Cardenas, Guillermo Andrés Cerquera
Jiacomini, Isabella Gizzi
Ramírez, Marcel Ivan
author2_role author
author
author
dc.contributor.author.fl_str_mv Alvarenga, Larissa Magalhães
Cardenas, Guillermo Andrés Cerquera
Jiacomini, Isabella Gizzi
Ramírez, Marcel Ivan
dc.subject.en.pt_BR.fl_str_mv Loxosceles
Brown Recluse Spider
Phospholipases D
Group II Phospholipases A2
Extracellular Vesicles
Envenomation
topic Loxosceles
Brown Recluse Spider
Phospholipases D
Group II Phospholipases A2
Extracellular Vesicles
Envenomation
Araña Reclusa Parda
Fosfolipasas A2 Grupo II
Vesículas Extracelulares
Envenenamiento
Araignée recluse brune
Group II Phospholipases A2
Vésicules extracellulaires
empoisonnement
Aranha Marrom Reclusa
Fosfolipases A2 do Grupo II
Vesículas Extracelulares
Envenenamento
dc.subject.es.pt_BR.fl_str_mv Araña Reclusa Parda
Fosfolipasas A2 Grupo II
Vesículas Extracelulares
Envenenamiento
dc.subject.fr.pt_BR.fl_str_mv Araignée recluse brune
Group II Phospholipases A2
Vésicules extracellulaires
empoisonnement
dc.subject.decs.pt_BR.fl_str_mv Aranha Marrom Reclusa
Fosfolipases A2 do Grupo II
Vesículas Extracelulares
Envenenamento
description Universidade Federal do Paraná. Departamento de Patologia Básica. Laboratório de Imunoquímica. Curitiba, PR, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-11-09T12:46:37Z
dc.date.available.fl_str_mv 2021-11-09T12:46:37Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALVARENGA, Larissa Magalhães et al. A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism. Toxicology Letters, n. 350, p. 202–212, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/49741
dc.identifier.issn.pt_BR.fl_str_mv 0378-4274
dc.identifier.doi.none.fl_str_mv 10.1016/j.toxlet.2021.07.014
identifier_str_mv ALVARENGA, Larissa Magalhães et al. A new insight into the cellular mechanisms of envenomation: Elucidating the role of extracellular vesicles in Loxoscelism. Toxicology Letters, n. 350, p. 202–212, 2021.
0378-4274
10.1016/j.toxlet.2021.07.014
url https://www.arca.fiocruz.br/handle/icict/49741
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/49741/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/49741/2/1-s2.0-S0378427421001946-OK.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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