Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 1992 |
| Outros Autores: | , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1590/s0074-02761992000400004 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/37455 |
Resumo: | Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Departamento de Biologia Celular e Genética. Rio de Janeiro, RJ, Brasil. |
| id |
CRUZ_682d263711c8822e37b2f0fa0f5ecd13 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/37455 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Tavares, Celso CruzGrimaldi Júnior, GabrielTraub-Csekö, Yara Marai2019-11-28T14:15:23Z2019-11-28T14:15:23Z1992TAVARES, Celso Cruz; GRIMALDI JR., Gabriel; TRAUB-CSEKO, Yara Maria. Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, n. 4, p. 447-486, Oct./Dec. 1992.0074-0276https://www.arca.fiocruz.br/handle/icict/3745510.1590/s0074-027619920004000041678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.LeishmaniaCromossomaCariotipagem molecularPFGEEletroforese de gel de campo pulsadoLocalização genéticaCromosomeMolecular karyotypeGene localizationPulsed-field gel electrophoresisLeishmaniaMolecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmaniainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Instituto de Biologia. Departamento de Biologia Celular e Genética. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica Biologia Celular. Rio de Janeiro, RJ, Brasil.The molecular karyotypes for 20 reference strains of species complexes of Leishmania were determined by contour-clamped homogeneous electric field (CHEF) electrophoresis. Determination of number/position of chromosome-sized bands and chromosomal DNA locations of housekeeping genes were the two criteria used for differentiating and classifying the Leishmania species. We have established two gel running conditions for optimal separation of chromosomes, which resolved DNA molecules as large as 2,500 kilobase pairs (kb). Chromosomes were polymorphic in number (22-30) and size (200-2,500 kb) of bands among members of five complexes of Leishmania. Although each stock had a distinct karyotype, in general the differences found between strains and/or species within each complex were not clear enough for parasite identification. However, each group showed a specific number of size-concordant DNA molecules, which allowed distinction among the Leishmania complex parasites. Clear differences between the Old and New world groups of parasites or among some New World Leishmania species were also apparent in relation to the chromosome locations of beta-tubulin genes. Based on these results as well as data from other published studies the potential of using DNA karyotype for identifying and classifying leishmanial field isolates is discussed.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/37455/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALYaraMariaT_Cseko_etal_IOC_1992.pdfYaraMariaT_Cseko_etal_IOC_1992.pdfapplication/pdf448633https://www.arca.fiocruz.br/bitstream/icict/37455/2/YaraMariaT_Cseko_etal_IOC_1992.pdf8c35e52a3754ed36d283a72b96a8601eMD52TEXTYaraMariaT_Cseko_etal_IOC_1992.pdf.txtYaraMariaT_Cseko_etal_IOC_1992.pdf.txtExtracted texttext/plain10https://www.arca.fiocruz.br/bitstream/icict/37455/3/YaraMariaT_Cseko_etal_IOC_1992.pdf.txt2c6eb67c8897d916ae47524b1a844d3fMD53icict/374552019-12-02 13:02:50.864oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-12-02T16:02:50Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania |
| title |
Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania |
| spellingShingle |
Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania Tavares, Celso Cruz Leishmania Cromossoma Cariotipagem molecular PFGE Eletroforese de gel de campo pulsado Localização genética Cromosome Molecular karyotype Gene localization Pulsed-field gel electrophoresis Leishmania |
| title_short |
Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania |
| title_full |
Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania |
| title_fullStr |
Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania |
| title_full_unstemmed |
Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania |
| title_sort |
Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania |
| author |
Tavares, Celso Cruz |
| author_facet |
Tavares, Celso Cruz Grimaldi Júnior, Gabriel Traub-Csekö, Yara Marai |
| author_role |
author |
| author2 |
Grimaldi Júnior, Gabriel Traub-Csekö, Yara Marai |
| author2_role |
author author |
| dc.contributor.author.fl_str_mv |
Tavares, Celso Cruz Grimaldi Júnior, Gabriel Traub-Csekö, Yara Marai |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania Cromossoma Cariotipagem molecular PFGE Eletroforese de gel de campo pulsado Localização genética |
| topic |
Leishmania Cromossoma Cariotipagem molecular PFGE Eletroforese de gel de campo pulsado Localização genética Cromosome Molecular karyotype Gene localization Pulsed-field gel electrophoresis Leishmania |
| dc.subject.en.pt_BR.fl_str_mv |
Cromosome Molecular karyotype Gene localization Pulsed-field gel electrophoresis Leishmania |
| description |
Universidade do Estado do Rio de Janeiro. Instituto de Biologia. Departamento de Biologia Celular e Genética. Rio de Janeiro, RJ, Brasil. |
| publishDate |
1992 |
| dc.date.issued.fl_str_mv |
1992 |
| dc.date.accessioned.fl_str_mv |
2019-11-28T14:15:23Z |
| dc.date.available.fl_str_mv |
2019-11-28T14:15:23Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
TAVARES, Celso Cruz; GRIMALDI JR., Gabriel; TRAUB-CSEKO, Yara Maria. Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, n. 4, p. 447-486, Oct./Dec. 1992. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/37455 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/s0074-02761992000400004 |
| dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
| identifier_str_mv |
TAVARES, Celso Cruz; GRIMALDI JR., Gabriel; TRAUB-CSEKO, Yara Maria. Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 87, n. 4, p. 447-486, Oct./Dec. 1992. 0074-0276 10.1590/s0074-02761992000400004 1678-8060 |
| url |
https://www.arca.fiocruz.br/handle/icict/37455 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
| publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/37455/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/37455/2/YaraMariaT_Cseko_etal_IOC_1992.pdf https://www.arca.fiocruz.br/bitstream/icict/37455/3/YaraMariaT_Cseko_etal_IOC_1992.pdf.txt |
| bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 8c35e52a3754ed36d283a72b96a8601e 2c6eb67c8897d916ae47524b1a844d3f |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1822792031672467456 |