Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania

Detalhes bibliográficos
Autor(a) principal: Tavares,Celso Cruz
Data de Publicação: 1992
Outros Autores: Grimaldi Junior,Gabriel, Traub Csekö,Yara Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000400004
Resumo: The molecular karyotypes for 20 reference strais of species complexes of Leishmania were determined by contour-clamped homogeneous eletric field (CHEF) electrosphoresis. Determination of number/position of chromosome-sized bands and chromosomal DNA locations of house-keeping genes were the two criteria used for differentiating and classifying the Leishmania species. We have established two gel running conditions of optimal separation of chromosomes, wich resolved DNA molecules as large as 2,500 kilobase pairs (kb). Chromosomes were polymorphic in number (22-30) and size (200-2,500 kb) of bands among members of five complexes of Leishmania. Although each stock had a distinct karyotype, in general the differences found between strains and/or species within each complex were not clear enough for parasite identification. However, each group showed a specific number of size-concordant DNA molecules, wich allowed distinction among the Leishmania complex parasites. Clear differences between the Old and New world groups of parasites or among some New World Leishmania species were also apparent in relation to the chromosome locations of beta-tubulin genes. Based on these results as well as data from other published studies the potencial of using DNA karyotype for identifying and classifying leishmanial field isolates is discussed.
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spelling Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of LeishmaniaLeishmaniachromosomemolecular karyotypegene localizationPFGE: pulsed-field gel electrophoresisThe molecular karyotypes for 20 reference strais of species complexes of Leishmania were determined by contour-clamped homogeneous eletric field (CHEF) electrosphoresis. Determination of number/position of chromosome-sized bands and chromosomal DNA locations of house-keeping genes were the two criteria used for differentiating and classifying the Leishmania species. We have established two gel running conditions of optimal separation of chromosomes, wich resolved DNA molecules as large as 2,500 kilobase pairs (kb). Chromosomes were polymorphic in number (22-30) and size (200-2,500 kb) of bands among members of five complexes of Leishmania. Although each stock had a distinct karyotype, in general the differences found between strains and/or species within each complex were not clear enough for parasite identification. However, each group showed a specific number of size-concordant DNA molecules, wich allowed distinction among the Leishmania complex parasites. Clear differences between the Old and New world groups of parasites or among some New World Leishmania species were also apparent in relation to the chromosome locations of beta-tubulin genes. Based on these results as well as data from other published studies the potencial of using DNA karyotype for identifying and classifying leishmanial field isolates is discussed.Instituto Oswaldo Cruz, Ministério da Saúde1992-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000400004Memórias do Instituto Oswaldo Cruz v.87 n.4 1992reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761992000400004info:eu-repo/semantics/openAccessTavares,Celso CruzGrimaldi Junior,GabrielTraub Csekö,Yara Mariaeng2020-04-25T17:46:47Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:04:45.699Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania
title Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania
spellingShingle Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania
Tavares,Celso Cruz
Leishmania
chromosome
molecular karyotype
gene localization
PFGE: pulsed-field gel electrophoresis
title_short Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania
title_full Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania
title_fullStr Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania
title_full_unstemmed Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania
title_sort Molecular karyotype analysis and mapping of housekeeping genes to chromosomes of selected species complexes of Leishmania
author Tavares,Celso Cruz
author_facet Tavares,Celso Cruz
Grimaldi Junior,Gabriel
Traub Csekö,Yara Maria
author_role author
author2 Grimaldi Junior,Gabriel
Traub Csekö,Yara Maria
author2_role author
author
dc.contributor.author.fl_str_mv Tavares,Celso Cruz
Grimaldi Junior,Gabriel
Traub Csekö,Yara Maria
dc.subject.por.fl_str_mv Leishmania
chromosome
molecular karyotype
gene localization
PFGE: pulsed-field gel electrophoresis
topic Leishmania
chromosome
molecular karyotype
gene localization
PFGE: pulsed-field gel electrophoresis
dc.description.none.fl_txt_mv The molecular karyotypes for 20 reference strais of species complexes of Leishmania were determined by contour-clamped homogeneous eletric field (CHEF) electrosphoresis. Determination of number/position of chromosome-sized bands and chromosomal DNA locations of house-keeping genes were the two criteria used for differentiating and classifying the Leishmania species. We have established two gel running conditions of optimal separation of chromosomes, wich resolved DNA molecules as large as 2,500 kilobase pairs (kb). Chromosomes were polymorphic in number (22-30) and size (200-2,500 kb) of bands among members of five complexes of Leishmania. Although each stock had a distinct karyotype, in general the differences found between strains and/or species within each complex were not clear enough for parasite identification. However, each group showed a specific number of size-concordant DNA molecules, wich allowed distinction among the Leishmania complex parasites. Clear differences between the Old and New world groups of parasites or among some New World Leishmania species were also apparent in relation to the chromosome locations of beta-tubulin genes. Based on these results as well as data from other published studies the potencial of using DNA karyotype for identifying and classifying leishmanial field isolates is discussed.
description The molecular karyotypes for 20 reference strais of species complexes of Leishmania were determined by contour-clamped homogeneous eletric field (CHEF) electrosphoresis. Determination of number/position of chromosome-sized bands and chromosomal DNA locations of house-keeping genes were the two criteria used for differentiating and classifying the Leishmania species. We have established two gel running conditions of optimal separation of chromosomes, wich resolved DNA molecules as large as 2,500 kilobase pairs (kb). Chromosomes were polymorphic in number (22-30) and size (200-2,500 kb) of bands among members of five complexes of Leishmania. Although each stock had a distinct karyotype, in general the differences found between strains and/or species within each complex were not clear enough for parasite identification. However, each group showed a specific number of size-concordant DNA molecules, wich allowed distinction among the Leishmania complex parasites. Clear differences between the Old and New world groups of parasites or among some New World Leishmania species were also apparent in relation to the chromosome locations of beta-tubulin genes. Based on these results as well as data from other published studies the potencial of using DNA karyotype for identifying and classifying leishmanial field isolates is discussed.
publishDate 1992
dc.date.none.fl_str_mv 1992-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000400004
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761992000400004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761992000400004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.87 n.4 1992
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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