Separation and Mapping of Chromosomes of Parasitic Protozoa

Detalhes bibliográficos
Autor(a) principal: Hernandez-Rivas,Rosaura
Data de Publicação: 1997
Outros Autores: Scherf,Artur
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600017
Resumo: Many protozoan parasites represent an important group of human pathogens. Pulsed Field Gradient Gel Electrophoresis (PFGE) analysis has been an important tool for fundamental genetic studies of parasites like Trypanosoma, Leishmania, Giardia or the human malaria parasite Plasmodium falciparum. We present PFGE conditions allowing a high resolution separation of chromosomes ranging from 500 to 4000 kb within a two day electrophoresis run. In addition, we present conditions for separating large chromosomes (2000-6000 kb) within 36 hr. We demontrate that the application of two dimentional PFGE (2D-PFGE) technique to parasite karyotypes is a very useful method for the analysis of dispersed gene families and comparative studies of the intrachomosomal genome organization
id FIOCRUZ-4_b72f393e4883d9ea89d579a6f0b58c1c
oai_identifier_str oai:scielo:S0074-02761997000600017
network_acronym_str FIOCRUZ-4
network_name_str Memórias do Instituto Oswaldo Cruz
spelling Separation and Mapping of Chromosomes of Parasitic ProtozoaPulsed Field Gradient Gel Electrophoresistwo dimensional PFGEprotozoan karyotypeschromosome mappingdispersed gene familiesMany protozoan parasites represent an important group of human pathogens. Pulsed Field Gradient Gel Electrophoresis (PFGE) analysis has been an important tool for fundamental genetic studies of parasites like Trypanosoma, Leishmania, Giardia or the human malaria parasite Plasmodium falciparum. We present PFGE conditions allowing a high resolution separation of chromosomes ranging from 500 to 4000 kb within a two day electrophoresis run. In addition, we present conditions for separating large chromosomes (2000-6000 kb) within 36 hr. We demontrate that the application of two dimentional PFGE (2D-PFGE) technique to parasite karyotypes is a very useful method for the analysis of dispersed gene families and comparative studies of the intrachomosomal genome organizationInstituto Oswaldo Cruz, Ministério da Saúde1997-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600017Memórias do Instituto Oswaldo Cruz v.92 n.6 1997reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761997000600017info:eu-repo/semantics/openAccessHernandez-Rivas,RosauraScherf,Artureng2020-04-25T17:47:46Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:07:43.85Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Separation and Mapping of Chromosomes of Parasitic Protozoa
title Separation and Mapping of Chromosomes of Parasitic Protozoa
spellingShingle Separation and Mapping of Chromosomes of Parasitic Protozoa
Hernandez-Rivas,Rosaura
Pulsed Field Gradient Gel Electrophoresis
two dimensional PFGE
protozoan karyotypes
chromosome mapping
dispersed gene families
title_short Separation and Mapping of Chromosomes of Parasitic Protozoa
title_full Separation and Mapping of Chromosomes of Parasitic Protozoa
title_fullStr Separation and Mapping of Chromosomes of Parasitic Protozoa
title_full_unstemmed Separation and Mapping of Chromosomes of Parasitic Protozoa
title_sort Separation and Mapping of Chromosomes of Parasitic Protozoa
author Hernandez-Rivas,Rosaura
author_facet Hernandez-Rivas,Rosaura
Scherf,Artur
author_role author
author2 Scherf,Artur
author2_role author
dc.contributor.author.fl_str_mv Hernandez-Rivas,Rosaura
Scherf,Artur
dc.subject.por.fl_str_mv Pulsed Field Gradient Gel Electrophoresis
two dimensional PFGE
protozoan karyotypes
chromosome mapping
dispersed gene families
topic Pulsed Field Gradient Gel Electrophoresis
two dimensional PFGE
protozoan karyotypes
chromosome mapping
dispersed gene families
dc.description.none.fl_txt_mv Many protozoan parasites represent an important group of human pathogens. Pulsed Field Gradient Gel Electrophoresis (PFGE) analysis has been an important tool for fundamental genetic studies of parasites like Trypanosoma, Leishmania, Giardia or the human malaria parasite Plasmodium falciparum. We present PFGE conditions allowing a high resolution separation of chromosomes ranging from 500 to 4000 kb within a two day electrophoresis run. In addition, we present conditions for separating large chromosomes (2000-6000 kb) within 36 hr. We demontrate that the application of two dimentional PFGE (2D-PFGE) technique to parasite karyotypes is a very useful method for the analysis of dispersed gene families and comparative studies of the intrachomosomal genome organization
description Many protozoan parasites represent an important group of human pathogens. Pulsed Field Gradient Gel Electrophoresis (PFGE) analysis has been an important tool for fundamental genetic studies of parasites like Trypanosoma, Leishmania, Giardia or the human malaria parasite Plasmodium falciparum. We present PFGE conditions allowing a high resolution separation of chromosomes ranging from 500 to 4000 kb within a two day electrophoresis run. In addition, we present conditions for separating large chromosomes (2000-6000 kb) within 36 hr. We demontrate that the application of two dimentional PFGE (2D-PFGE) technique to parasite karyotypes is a very useful method for the analysis of dispersed gene families and comparative studies of the intrachomosomal genome organization
publishDate 1997
dc.date.none.fl_str_mv 1997-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600017
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600017
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761997000600017
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.92 n.6 1997
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
_version_ 1669937671568359424

Registros relacionados