The impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegypti

Detalhes bibliográficos
Autor(a) principal: Pacidônio, Etiene Casagrande
Data de Publicação: 2017
Outros Autores: Caragata, Eric Pearce, Alves, Debora Magalhães, Marques, João Trindade, Moreira, Luciano Andrade
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/26045
Resumo: 2018-04-23
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spelling Pacidônio, Etiene CasagrandeCaragata, Eric PearceAlves, Debora MagalhãesMarques, João TrindadeMoreira, Luciano Andrade2018-04-23T19:14:52Z2018-04-23T19:14:52Z2017PACIDÔNIO, Etiene Casagrande et al. The impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegypti. Parasit Vectors, v. 10, n. 1, art.296, 2017.1756-3305https://www.arca.fiocruz.br/handle/icict/2604510.1186/s13071-017-2236-zengBioMed CentralWolbachiaDengue virusAedes aegyptiWolbachiaDengue virusAedes aegyptiVertical transmissionArbovirusThe impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegyptiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2018-04-23Fundação Oswaldo Cruz. Instituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, Brazil/Monash University. School of Biological Sciences. Clayton, Melbourne, VIC AustraliaFundação Oswaldo Cruz. Instituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquimica e Imunologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Instituto René Rachou. Grupo Mosquitos Vetores: Endossimbiontes e Interação Patógeno Vetor. Belo Horizonte, MG, BrazilBackground: Wolbachia pipientis is a common endosymbiotic bacterium of arthropods that strongly inhibits dengue virus (DENV) infection and transmission in the primary vector, the mosquito Aedes aegypti. For that reason, Wolbachia-infected Ae. aegypti are currently being released into the field as part of a novel strategy to reduce DENV transmission. However, there is evidence that DENV can be transmitted vertically from mother to progeny, and this may help the virus persist in nature in the absence of regular human transmission. The effect of Wolbachia infection on this process had not previously been examined. Results: We challenged Ae. aegypti with different Brazilian DENV isolates either by oral feeding or intrathoracic injection to ensure disseminated infection. We examined the effect of Wolbachia infection on the prevalence of DENV infection, and viral load in the ovaries. For orally infected mosquitoes, Wolbachia decreased the prevalence of infection by 71.29%, but there was no such effect when the virus was injected. Interestingly, regardless of the method of infection, Wolbachia infection strongly reduced DENV load in the ovaries. We then looked at the effect of Wolbachia on vertical transmission, where we observed only very low rates of vertical transmission. There was a trend towards lower rates in the presence of Wolbachia, with overall maximum likelihood estimate of infection rates of 5.04 per 1000 larvae for mosquitoes without Wolbachia, and 1.93 per 1000 larvae for Wolbachia-infected mosquitoes, after DENV injection. However, this effect was not statistically significant. Conclusions: Our data support the idea that vertical transmission of DENV is rare in nature, even in the absence of Wolbachia. Indeed, we observed that vertical transmission rates were low even when the midgut barrier was bypassed, which might help to explain why we only observed a trend towards lower vertical transmission rates in the presence of Wolbachia. Nevertheless, the low prevalence of disseminated DENV infection and lower DENV load in the ovaries supports the hypothesis that the presence of Wolbachia in Ae. aegypti would have an effect on the vertical transmission of DENV in the field.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/26045/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALThe impact of Wolbachia infection on the rate of vertical .pdfThe impact of Wolbachia infection on the rate of vertical .pdfapplication/pdf6817475https://www.arca.fiocruz.br/bitstream/icict/26045/2/The%20impact%20of%20Wolbachia%20infection%20on%20the%20rate%20of%20vertical%20.pdf27bd8ed50aa5178fb75a551ab7522976MD52TEXTThe impact of Wolbachia infection on the rate of vertical .pdf.txtThe impact of Wolbachia infection on the rate of vertical 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dc.title.pt_BR.fl_str_mv The impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegypti
title The impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegypti
spellingShingle The impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegypti
Pacidônio, Etiene Casagrande
Wolbachia
Dengue virus
Aedes aegypti
Wolbachia
Dengue virus
Aedes aegypti
Vertical transmission
Arbovirus
title_short The impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegypti
title_full The impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegypti
title_fullStr The impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegypti
title_full_unstemmed The impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegypti
title_sort The impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegypti
author Pacidônio, Etiene Casagrande
author_facet Pacidônio, Etiene Casagrande
Caragata, Eric Pearce
Alves, Debora Magalhães
Marques, João Trindade
Moreira, Luciano Andrade
author_role author
author2 Caragata, Eric Pearce
Alves, Debora Magalhães
Marques, João Trindade
Moreira, Luciano Andrade
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Pacidônio, Etiene Casagrande
Caragata, Eric Pearce
Alves, Debora Magalhães
Marques, João Trindade
Moreira, Luciano Andrade
dc.subject.other.pt_BR.fl_str_mv Wolbachia
Dengue virus
Aedes aegypti
topic Wolbachia
Dengue virus
Aedes aegypti
Wolbachia
Dengue virus
Aedes aegypti
Vertical transmission
Arbovirus
dc.subject.en.pt_BR.fl_str_mv Wolbachia
Dengue virus
Aedes aegypti
Vertical transmission
Arbovirus
description 2018-04-23
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-04-23T19:14:52Z
dc.date.available.fl_str_mv 2018-04-23T19:14:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PACIDÔNIO, Etiene Casagrande et al. The impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegypti. Parasit Vectors, v. 10, n. 1, art.296, 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/26045
dc.identifier.issn.pt_BR.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/s13071-017-2236-z
identifier_str_mv PACIDÔNIO, Etiene Casagrande et al. The impact of Wolbachia infection on the rate of vertical transmission of dengue virus in Brazilian Aedes aegypti. Parasit Vectors, v. 10, n. 1, art.296, 2017.
1756-3305
10.1186/s13071-017-2236-z
url https://www.arca.fiocruz.br/handle/icict/26045
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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