Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004

Detalhes bibliográficos
Autor(a) principal: Costa, Filipe Anibal Carvalho
Data de Publicação: 2006
Outros Autores: Assis, Rosane Maria, Fialho, Alexandre Madi, Bóia, Márcio Neves, Alves, Daniele Pires Dias, Martins, Carolina Maria Miranda de Assis, Leite, José Paulo Gagliardi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36621
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
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spelling Costa, Filipe Anibal CarvalhoAssis, Rosane MariaFialho, Alexandre MadiBóia, Márcio NevesAlves, Daniele Pires DiasMartins, Carolina Maria Miranda de AssisLeite, José Paulo Gagliardi2019-10-22T12:27:55Z2019-10-22T12:27:55Z2006COSTA, Filipe Anibal Carvalho et al. Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 3, p. 291-294, May 2006.0074-0276https://www.arca.fiocruz.br/handle/icict/3662110.1590/S0074-027620060003000121678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzGastroenteriteHospitalizaçãoRotavirus AGenótiposRio de JaneiroBrasilGastroenteritisHospitalizationRotavirus AGenotypesRio de JaneiroBrazilDetection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.Hospital Municipal Souza Aguiar. Rio de Janeiro, RJ, Brasil.Hospital Municipal Salles Netto. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.Rotavirus is a major cause of infantile acute diarrhea, causing about 440,000 deaths per year, mainly in developing countries. The World Health Organization has been recommending the assessment of rotavirus burden and strain characterization as part of the strategies of immunization programs against this pathogen. In this context, a prospective study was made on a sample of 134 children with acute diarrhea and severe dehydration admitted to venous fluid therapy in two state hospitals in Rio de Janeiro, Brazil, from February to September 2004. Rotavirus where detected by polyacrylamide gel electrophoresis (PAGE) and by an enzyme-linked immunoassay to rotavirus and adenovirus (EIARA) in 48% of the children. Positive samples for group A rotavirus (n = 65) were analyzed by reverse transcription/heminested multiplex polymerase chain reaction to determine the frequency of G and [P] genotypes and, from these, 64 samples could be typed. The most frequent G genotype was G1 (58%) followed by G9 (40%). One mixed infection (G1/G9) was detected. The only [P] genotype identified was P[8]. In order to estimate the rotavirus infection frequency in children who acquired diarrhea as hospital infection in those hospitals, we studied 24 patients, detecting the pathogen in 41% of them. This data suggest that genotype G9 is an important genotype in Rio de Janeiro, with implications to the future strategies of vaccination against rotavirus, reinforcing the need of continuous monitoring of circulating strains of the pathogen, in a surveillance context.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/36621/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarcioNBoia_JosePGLeite_etal_IOC_2006.pdfMarcioNBoia_JosePGLeite_etal_IOC_2006.pdfapplication/pdf419470https://www.arca.fiocruz.br/bitstream/icict/36621/2/MarcioNBoia_JosePGLeite_etal_IOC_2006.pdf7cca658b3d0b3686444c1919b7b972fdMD52TEXTMarcioNBoia_JosePGLeite_etal_IOC_2006.pdf.txtMarcioNBoia_JosePGLeite_etal_IOC_2006.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004
title Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004
spellingShingle Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004
Costa, Filipe Anibal Carvalho
Gastroenterite
Hospitalização
Rotavirus A
Genótipos
Rio de Janeiro
Brasil
Gastroenteritis
Hospitalization
Rotavirus A
Genotypes
Rio de Janeiro
Brazil
title_short Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004
title_full Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004
title_fullStr Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004
title_full_unstemmed Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004
title_sort Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004
author Costa, Filipe Anibal Carvalho
author_facet Costa, Filipe Anibal Carvalho
Assis, Rosane Maria
Fialho, Alexandre Madi
Bóia, Márcio Neves
Alves, Daniele Pires Dias
Martins, Carolina Maria Miranda de Assis
Leite, José Paulo Gagliardi
author_role author
author2 Assis, Rosane Maria
Fialho, Alexandre Madi
Bóia, Márcio Neves
Alves, Daniele Pires Dias
Martins, Carolina Maria Miranda de Assis
Leite, José Paulo Gagliardi
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Filipe Anibal Carvalho
Assis, Rosane Maria
Fialho, Alexandre Madi
Bóia, Márcio Neves
Alves, Daniele Pires Dias
Martins, Carolina Maria Miranda de Assis
Leite, José Paulo Gagliardi
dc.subject.other.pt_BR.fl_str_mv Gastroenterite
Hospitalização
Rotavirus A
Genótipos
Rio de Janeiro
Brasil
topic Gastroenterite
Hospitalização
Rotavirus A
Genótipos
Rio de Janeiro
Brasil
Gastroenteritis
Hospitalization
Rotavirus A
Genotypes
Rio de Janeiro
Brazil
dc.subject.en.pt_BR.fl_str_mv Gastroenteritis
Hospitalization
Rotavirus A
Genotypes
Rio de Janeiro
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Virologia Comparada. Rio de Janeiro, RJ, Brasil.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2019-10-22T12:27:55Z
dc.date.available.fl_str_mv 2019-10-22T12:27:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Filipe Anibal Carvalho et al. Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 3, p. 291-294, May 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36621
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762006000300012
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv COSTA, Filipe Anibal Carvalho et al. Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 3, p. 291-294, May 2006.
0074-0276
10.1590/S0074-02762006000300012
1678-8060
url https://www.arca.fiocruz.br/handle/icict/36621
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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