Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals.

Detalhes bibliográficos
Autor(a) principal: Miranda, Aline Cristina Andrade Mota
Data de Publicação: 2013
Outros Autores: Barreto, Fernanda Khouri, Amarante, Maria Fernanda de Castro, Batista, Everton da Silva, Cunha, Joana Paixão Monteiro, Vallve, Maria de Lourdes Farre, Castro Filho, Bernardo Galvão, Alcantara, Luiz Carlos Júnior
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8767
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil
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spelling Miranda, Aline Cristina Andrade MotaBarreto, Fernanda KhouriAmarante, Maria Fernanda de CastroBatista, Everton da SilvaCunha, Joana Paixão MonteiroVallve, Maria de Lourdes FarreCastro Filho, Bernardo GalvãoAlcantara, Luiz Carlos Júnior2014-11-07T17:45:32Z2014-11-07T17:45:32Z2013MIRANDA, A. C. A. M. et al. Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals. Virology Journal, v. 10, p. 75, 2013.1743-422Xhttps://www.arca.fiocruz.br/handle/icict/876710.1186/1743-422X-10-75engBioMed CentralMolecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilCancer Institute. National Institutes of Health. Bethesda, MD, USAFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, BrasilEscola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia Experimental. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilBACKGROUND: Human T-cell Leukemia Virus type 1 (HTLV-1) is the etiological agent of tropical spastic paraparesis/HTLV-associated myelopathy (HAM/TSP) that can be identified in around 0.25%-3.8% of the infected population. Disease progression can be monitored by the proviral load and may depend on genetic factors, however, it is not well understood why some HTLV-1 infected people develop the disease while others do not. The present study attempts to assess the molecular diversity of gp46 glycoprotein in HAM/TSP patients and Health Carrier (HC) individuals. METHODS: Blood samples were collected from 10 individuals, and DNA was extracted from PBMCs to measure the HTLV-1 proviral load. The gp46 coding sequences were amplified PCR, cloned and sequenced. The molecular characterization was performed using bioinformatics tools. RESULTS: The median HTLV-1 proviral load of HC (n = 5) and HAM/TSP (n = 5) patients was similar (average 316,227 copies/106 PBMCs). The gp46 molecular characterization of 146 clones (70 HC and 76 HAM/TSP) revealed an overall diversity, within HC and HAM/TSP clones, of 0.4% and 0.6%, respectively. Five frequent mutations were detected among groups (HAM/TSP and HC clone sequences). A single amino acid (aa) substitution (S35L) was exclusive for the HC group, and three gp46 substitutions (F14S, N42H, G72S) were exclusive for the HAM/TSP group. The remaining frequent mutation (V247I) was present in both groups (p = 0.0014). The in silico protein analysis revealed that the mutated alleles F14S and N42H represent more hydrophilic and flexible protein domains that are likely to be less antigenic. The Receptor Binding Domain is quite variable in the HAM/TSP group. Two other domains (aa 53-75 and 175-209) that contain multiple linear T-cell epitopes showed genetic diversity in both HAM/TSP and HC groups. Further analysis revealed 27 and 13 T-cell epitopes for class I HLA alleles and class II HLA alleles, when analyzing the entire gp46. CONCLUSIONS: The most common gp46 mutations were not associated clinical status because they were found in only one individual, except for the V247I mutation, that was found at viral clones from HAM/TSP ad HC individuals. Because of this, we cannot associate any of the gp46 found mutations with the clinical profile.HTLV-1HAM/TSPGp46MutationPortador Sadio/virologiaProdutos do Gene env/genéticaVírus 1 Linfotrópico T Humano/genéticaParaparesia Espástica Tropical/virologiaProteínas Oncogênicas de Retroviridae/genéticaAdultoIdosoSequência de AminoácidosPortador Sadio/imunologiaEpitopos de Linfócito T/químicaProdutos do Gene env/químicaFemininoVírus 1 Linfotrópico T Humano/químicaVírus 1 Linfotrópico T Humano/isolamento & purificaçãoHumanosMasculinoMeia-IdadeDados de Sequência MolecularMutaçãoParaparesia Espástica Tropical/imunologiaEstrutura Terciária de ProteínaProteínas Oncogênicas de Retroviridae/imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8767/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALMiranda ACAM Molecular ....pdfMiranda ACAM Molecular ....pdfapplication/pdf959097https://www.arca.fiocruz.br/bitstream/icict/8767/2/Miranda%20ACAM%20Molecular%20....pdfcbda3547c79c7af5f70c9cf2ab8c84bdMD52TEXTMiranda ACAM Molecular ....pdf.txtMiranda ACAM Molecular ....pdf.txtExtracted texttext/plain45016https://www.arca.fiocruz.br/bitstream/icict/8767/3/Miranda%20ACAM%20Molecular%20....pdf.txtec299582af2f0a1e2f15cb53edf6edd3MD53icict/87672023-03-15 14:32:56.359oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:32:56Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals.
title Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals.
spellingShingle Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals.
Miranda, Aline Cristina Andrade Mota
HTLV-1
HAM/TSP
Gp46
Mutation
Portador Sadio/virologia
Produtos do Gene env/genética
Vírus 1 Linfotrópico T Humano/genética
Paraparesia Espástica Tropical/virologia
Proteínas Oncogênicas de Retroviridae/genética
Adulto
Idoso
Sequência de Aminoácidos
Portador Sadio/imunologia
Epitopos de Linfócito T/química
Produtos do Gene env/química
Feminino
Vírus 1 Linfotrópico T Humano/química
Vírus 1 Linfotrópico T Humano/isolamento & purificação
Humanos
Masculino
Meia-Idade
Dados de Sequência Molecular
Mutação
Paraparesia Espástica Tropical/imunologia
Estrutura Terciária de Proteína
Proteínas Oncogênicas de Retroviridae/imunologia
title_short Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals.
title_full Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals.
title_fullStr Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals.
title_full_unstemmed Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals.
title_sort Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals.
author Miranda, Aline Cristina Andrade Mota
author_facet Miranda, Aline Cristina Andrade Mota
Barreto, Fernanda Khouri
Amarante, Maria Fernanda de Castro
Batista, Everton da Silva
Cunha, Joana Paixão Monteiro
Vallve, Maria de Lourdes Farre
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
author_role author
author2 Barreto, Fernanda Khouri
Amarante, Maria Fernanda de Castro
Batista, Everton da Silva
Cunha, Joana Paixão Monteiro
Vallve, Maria de Lourdes Farre
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Miranda, Aline Cristina Andrade Mota
Barreto, Fernanda Khouri
Amarante, Maria Fernanda de Castro
Batista, Everton da Silva
Cunha, Joana Paixão Monteiro
Vallve, Maria de Lourdes Farre
Castro Filho, Bernardo Galvão
Alcantara, Luiz Carlos Júnior
dc.subject.en.pt_BR.fl_str_mv HTLV-1
HAM/TSP
Gp46
Mutation
topic HTLV-1
HAM/TSP
Gp46
Mutation
Portador Sadio/virologia
Produtos do Gene env/genética
Vírus 1 Linfotrópico T Humano/genética
Paraparesia Espástica Tropical/virologia
Proteínas Oncogênicas de Retroviridae/genética
Adulto
Idoso
Sequência de Aminoácidos
Portador Sadio/imunologia
Epitopos de Linfócito T/química
Produtos do Gene env/química
Feminino
Vírus 1 Linfotrópico T Humano/química
Vírus 1 Linfotrópico T Humano/isolamento & purificação
Humanos
Masculino
Meia-Idade
Dados de Sequência Molecular
Mutação
Paraparesia Espástica Tropical/imunologia
Estrutura Terciária de Proteína
Proteínas Oncogênicas de Retroviridae/imunologia
dc.subject.decs.pt_BR.fl_str_mv Portador Sadio/virologia
Produtos do Gene env/genética
Vírus 1 Linfotrópico T Humano/genética
Paraparesia Espástica Tropical/virologia
Proteínas Oncogênicas de Retroviridae/genética
Adulto
Idoso
Sequência de Aminoácidos
Portador Sadio/imunologia
Epitopos de Linfócito T/química
Produtos do Gene env/química
Feminino
Vírus 1 Linfotrópico T Humano/química
Vírus 1 Linfotrópico T Humano/isolamento & purificação
Humanos
Masculino
Meia-Idade
Dados de Sequência Molecular
Mutação
Paraparesia Espástica Tropical/imunologia
Estrutura Terciária de Proteína
Proteínas Oncogênicas de Retroviridae/imunologia
description Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-11-07T17:45:32Z
dc.date.available.fl_str_mv 2014-11-07T17:45:32Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MIRANDA, A. C. A. M. et al. Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals. Virology Journal, v. 10, p. 75, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8767
dc.identifier.issn.none.fl_str_mv 1743-422X
dc.identifier.doi.none.fl_str_mv 10.1186/1743-422X-10-75
identifier_str_mv MIRANDA, A. C. A. M. et al. Molecular characterization of HTLV-1 gp46 glycoprotein from health carriers and HAM/TSP infected individuals. Virology Journal, v. 10, p. 75, 2013.
1743-422X
10.1186/1743-422X-10-75
url https://www.arca.fiocruz.br/handle/icict/8767
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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