Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct

Detalhes bibliográficos
Autor(a) principal: Albuquerque, Priscila C.
Data de Publicação: 2014
Outros Autores: Fonseca, Fernanda L., Dutra, Fabianno F., Bozza, Marcelo T., Frases, Susana, Casadevall, Arturo, Rodrigues, Marcio L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59631
Resumo: Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil.
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spelling Albuquerque, Priscila C.Fonseca, Fernanda L.Dutra, Fabianno F.Bozza, Marcelo T.Frases, SusanaCasadevall, ArturoRodrigues, Marcio L.2023-07-18T04:21:14Z2023-07-18T04:21:14Z2014ALBUQUERQUE, Priscila C. et al. Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct. Future Microbiology, v. 9, n. 2, p. 147-161, 2014.1746-0913https://www.arca.fiocruz.br/handle/icict/5963110.2217/FMB.13.1631746-0921engFuture MedicineAnimalsCryptococcosisCryptococcus neoformansCytokinesFungal CapsulesMacrophagesMiceMice, Inbred C57BLMice, KnockoutMolecular WeightPolysaccharidesProtein BindingSubcellular FractionsCryptococcus neoformansGlucuronoxylomannanHost responseMolecular massPolysaccharidesCryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinctinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil.Aims: Glucuronoxylomannan (GXM) is the major polysaccharide component of Cryptococcus neoformans. We evaluated in this study whether GXM fractions of different molecular masses were functionally distinct. Materials & methods: GXM samples isolated from C. neoformans cultures were fractionated to generate polysaccharide preparations differing in molecular mass. These fractions were used in experiments focused on the association of GXM with cell wall components of C. neoformans, as well as on the interaction of the polysaccharide with host cells. Results & conclusion: GXM fractions of variable molecular masses bound to the surface of a C. neoformans acapsular mutant in a punctate pattern that is in contrast to the usual annular pattern of surface coating observed when GXM samples containing the full molecular mass range were used. The polysaccharide samples were also significantly different in their ability to stimulate cytokine production by host cells. Our findings indicate that GXM fractions are functionally distinct depending on their mass.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59631/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINAL10.2217_fmb.13.163.pdfapplication/pdf1102913https://www.arca.fiocruz.br/bitstream/icict/59631/2/10.2217_fmb.13.163.pdfd4ff7f0e9a14c68b64611a0c405b087bMD52icict/596312023-07-18 21:53:06.55oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-19T00:53:06Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct
title Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct
spellingShingle Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct
Albuquerque, Priscila C.
Animals
Cryptococcosis
Cryptococcus neoformans
Cytokines
Fungal Capsules
Macrophages
Mice
Mice, Inbred C57BL
Mice, Knockout
Molecular Weight
Polysaccharides
Protein Binding
Subcellular Fractions
Cryptococcus neoformans
Glucuronoxylomannan
Host response
Molecular mass
Polysaccharides
title_short Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct
title_full Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct
title_fullStr Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct
title_full_unstemmed Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct
title_sort Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct
author Albuquerque, Priscila C.
author_facet Albuquerque, Priscila C.
Fonseca, Fernanda L.
Dutra, Fabianno F.
Bozza, Marcelo T.
Frases, Susana
Casadevall, Arturo
Rodrigues, Marcio L.
author_role author
author2 Fonseca, Fernanda L.
Dutra, Fabianno F.
Bozza, Marcelo T.
Frases, Susana
Casadevall, Arturo
Rodrigues, Marcio L.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Albuquerque, Priscila C.
Fonseca, Fernanda L.
Dutra, Fabianno F.
Bozza, Marcelo T.
Frases, Susana
Casadevall, Arturo
Rodrigues, Marcio L.
dc.subject.mesh.en_US.fl_str_mv Animals
Cryptococcosis
Cryptococcus neoformans
Cytokines
Fungal Capsules
Macrophages
Mice
Mice, Inbred C57BL
Mice, Knockout
Molecular Weight
Polysaccharides
Protein Binding
Subcellular Fractions
topic Animals
Cryptococcosis
Cryptococcus neoformans
Cytokines
Fungal Capsules
Macrophages
Mice
Mice, Inbred C57BL
Mice, Knockout
Molecular Weight
Polysaccharides
Protein Binding
Subcellular Fractions
Cryptococcus neoformans
Glucuronoxylomannan
Host response
Molecular mass
Polysaccharides
dc.subject.en.en_US.fl_str_mv Cryptococcus neoformans
Glucuronoxylomannan
Host response
Molecular mass
Polysaccharides
description Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brazil / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Gões. Rio de Janeiro, RJ, Brazil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2023-07-18T04:21:14Z
dc.date.available.fl_str_mv 2023-07-18T04:21:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALBUQUERQUE, Priscila C. et al. Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct. Future Microbiology, v. 9, n. 2, p. 147-161, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59631
dc.identifier.issn.en_US.fl_str_mv 1746-0913
dc.identifier.doi.none.fl_str_mv 10.2217/FMB.13.163
dc.identifier.eissn.none.fl_str_mv 1746-0921
identifier_str_mv ALBUQUERQUE, Priscila C. et al. Cryptococcus neoformans glucuronoxylomannan fractions of different molecular masses are functionally distinct. Future Microbiology, v. 9, n. 2, p. 147-161, 2014.
1746-0913
10.2217/FMB.13.163
1746-0921
url https://www.arca.fiocruz.br/handle/icict/59631
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Future Medicine
publisher.none.fl_str_mv Future Medicine
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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