Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection

Detalhes bibliográficos
Autor(a) principal: Cordero, Radames J. B.
Data de Publicação: 2016
Outros Autores: Liedke, Susie Coutinho, Araújo, Glauber R. de S., Martinez, Luis R., Nimrichter, Leonardo, Frases, Susana, Peralta, José Mauro, Casadevall, Arturo, Rodrigues, Marcio L., Nosanchuk, Joshua D., Guimaraes, Allan J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/59628
Resumo: Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, USA.
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spelling Cordero, Radames J. B.Liedke, Susie CoutinhoAraújo, Glauber R. de S.Martinez, Luis R.Nimrichter, LeonardoFrases, SusanaPeralta, José MauroCasadevall, ArturoRodrigues, Marcio L.Nosanchuk, Joshua D.Guimaraes, Allan J.2023-07-18T03:17:59Z2023-07-18T03:17:59Z2016CORDERO, Radames J. B. et al. Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection. Scientific Reports, v. 6, p. 1-16, Feb. 2016.2045-2322https://www.arca.fiocruz.br/handle/icict/5962810.1038/SREP217652045-2322engNature Publishing GroupAnimalsCoinfectionCryptococcosisCryptococcus neoformansFemaleFungal PolysaccharidesHistoplasmaHistoplasmosisMacrophages, PeritonealMice, Inbred C57BLMicrobial InteractionsVirulenceHistoplasma capsulatumCryptococcus neoformansInvasive fungal-infectionsCutaneous cryptococcosisMycosesMicrobial interactionsPpolysaccharideFungal polysaccharidesEnhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDepartment of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, USA.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brazil.Department of Biomedical Sciences. College of Osteopathic Medicine. New York Institute of Technology. Old Westbury, USA.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Glicobiologia de Eucariotos. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brazil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Rio de Janeiro, RJ, Brazil.Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, USA.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Laboratório de Biologia Celular de Leveduras Patogênicas. Rio de Janeiro, RJ, Brazil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde. Rio de Janeiro, RJ, Brasil.Department of Microbiology and Immunology. Albert Einstein College of Medicine. Yeshiva University. Bronx, USA / Department of Medicine (Division of Infectious Diseases). Albert Einstein College of Medicine. Yeshiva University. Bronx, USA.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Rio de Janeiro, RJ, Brazil.Cryptococcus neoformans (Cn) and Histoplasma capsulatum (Hc) co-exist in the environment and occasionally co-infect individuals, which can lead to severe disease/lethal outcomes. We investigated specific interactions between Cn-Hc to determine the impact of synchronous infection in virulence and disease. Co-infected mice had significantly higher mortality than infection with either species or acapsular Cn-Hc. Coating of Hc with cryptococcal glycans (Cn-gly) resulted in higher pulmonary fungal burden in co-infected animals relative to control. Co-cultivation or addition of Cn-gly resulted in enhanced pellicle formation with a hybrid polysaccharide matrix with higher reactivity to GXM mAbs. Transfer and incorporation of Cn polysaccharide onto Hc surface was time and temperature dependent. Cn-gly transfer altered the zeta potential of Hc and was associated with increased resistance to phagocytosis and killing by macrophages. Mice infected with Hc and subsequently injected with purified Cn-gly died significantly more rapidly than Hc alone infected, establishing the precedent that virulence factors from one fungus can enhance the virulence of unrelated species. These findings suggest a new mechanism of microbial interaction involving the transfer of virulence traits that translates into enhanced lethality during mixed fungal infections and highlights the importance of studying heterogeneous microbial populations in the setting of infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/59628/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINAL10.1038_srep21765.pdfapplication/pdf2263534https://www.arca.fiocruz.br/bitstream/icict/59628/2/10.1038_srep21765.pdf0b7cdd0e46850191254a5b5ef03c52ccMD52icict/596282023-07-18 22:14:34.833oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-07-19T01:14:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection
title Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection
spellingShingle Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection
Cordero, Radames J. B.
Animals
Coinfection
Cryptococcosis
Cryptococcus neoformans
Female
Fungal Polysaccharides
Histoplasma
Histoplasmosis
Macrophages, Peritoneal
Mice, Inbred C57BL
Microbial Interactions
Virulence
Histoplasma capsulatum
Cryptococcus neoformans
Invasive fungal-infections
Cutaneous cryptococcosis
Mycoses
Microbial interactions
Ppolysaccharide
Fungal polysaccharides
title_short Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection
title_full Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection
title_fullStr Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection
title_full_unstemmed Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection
title_sort Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection
author Cordero, Radames J. B.
author_facet Cordero, Radames J. B.
Liedke, Susie Coutinho
Araújo, Glauber R. de S.
Martinez, Luis R.
Nimrichter, Leonardo
Frases, Susana
Peralta, José Mauro
Casadevall, Arturo
Rodrigues, Marcio L.
Nosanchuk, Joshua D.
Guimaraes, Allan J.
author_role author
author2 Liedke, Susie Coutinho
Araújo, Glauber R. de S.
Martinez, Luis R.
Nimrichter, Leonardo
Frases, Susana
Peralta, José Mauro
Casadevall, Arturo
Rodrigues, Marcio L.
Nosanchuk, Joshua D.
Guimaraes, Allan J.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Cordero, Radames J. B.
Liedke, Susie Coutinho
Araújo, Glauber R. de S.
Martinez, Luis R.
Nimrichter, Leonardo
Frases, Susana
Peralta, José Mauro
Casadevall, Arturo
Rodrigues, Marcio L.
Nosanchuk, Joshua D.
Guimaraes, Allan J.
dc.subject.mesh.en_US.fl_str_mv Animals
Coinfection
Cryptococcosis
Cryptococcus neoformans
Female
Fungal Polysaccharides
Histoplasma
Histoplasmosis
Macrophages, Peritoneal
Mice, Inbred C57BL
Microbial Interactions
Virulence
topic Animals
Coinfection
Cryptococcosis
Cryptococcus neoformans
Female
Fungal Polysaccharides
Histoplasma
Histoplasmosis
Macrophages, Peritoneal
Mice, Inbred C57BL
Microbial Interactions
Virulence
Histoplasma capsulatum
Cryptococcus neoformans
Invasive fungal-infections
Cutaneous cryptococcosis
Mycoses
Microbial interactions
Ppolysaccharide
Fungal polysaccharides
dc.subject.en.en_US.fl_str_mv Histoplasma capsulatum
Cryptococcus neoformans
Invasive fungal-infections
Cutaneous cryptococcosis
Mycoses
Microbial interactions
Ppolysaccharide
Fungal polysaccharides
description Department of Molecular Microbiology and Immunology. Johns Hopkins Bloomberg School of Public Health. Baltimore, USA.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2023-07-18T03:17:59Z
dc.date.available.fl_str_mv 2023-07-18T03:17:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CORDERO, Radames J. B. et al. Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection. Scientific Reports, v. 6, p. 1-16, Feb. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/59628
dc.identifier.issn.en_US.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/SREP21765
dc.identifier.eissn.none.fl_str_mv 2045-2322
identifier_str_mv CORDERO, Radames J. B. et al. Enhanced virulence of Histoplasma capsulatum through transfer and surface incorporation of glycans from Cryptococcus neoformans during co-infection. Scientific Reports, v. 6, p. 1-16, Feb. 2016.
2045-2322
10.1038/SREP21765
url https://www.arca.fiocruz.br/handle/icict/59628
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/59628/1/license.txt
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