Comparative Evaluation of Lesion Development, Tissue Damage, and Cytokine Expression in Golden Hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrations

Detalhes bibliográficos
Autor(a) principal: Romão, Raquel P. Ribeiro
Data de Publicação: 2014
Outros Autores: Moreira, Otacilio C., Osorio, Elvia Yaneth, Finkelstein, Lea Cysne, Silva, Adriano Gomes, Valverde, Joanna G, Pirmez, Claude, Cruz, Alda Maria da, Pinto, Eduardo Fonseca
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10191
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.
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spelling Romão, Raquel P. RibeiroMoreira, Otacilio C.Osorio, Elvia YanethFinkelstein, Lea CysneSilva, Adriano GomesValverde, Joanna GPirmez, ClaudeCruz, Alda Maria daPinto, Eduardo Fonseca2015-05-04T17:07:26Z2015-05-04T17:07:26Z2014ROMÃO, Raquel P. Ribeiro et al. Comparative evaluation of lesion developmente, tissue damage, and cytokine expression in golden hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrations. Infect. Immun., v.82, n.12, p.5203-5213, 2014.0019-95671098-5522https://www.arca.fiocruz.br/handle/icict/1019110.1128/IAI.02083-14porAmerican Society for MicrobiologyLeishmaniaMesocricetusLeishmaniaGolden HamstersLeishmaniaMesocricetusMesocricetusLeishmaniaComparative Evaluation of Lesion Development, Tissue Damage, and Cytokine Expression in Golden Hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.University of Texas Medical Branch. Department of Internal Medicine. Galveston, Texas, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.The golden hamster (Mesocricetus auratus) is a susceptible model to Leishmania (Viannia) spp.; however, available studies employ different infection protocols, which account for clinical and pathological presentation differences. Herein, L. (V.) braziliensis preparations were standardized to contain 104, 105, or 106 parasites to determine an optimal inoculum that ensured cutaneous lesions without causing a disseminated infection in hamsters. Lesion development was followed for 105 days by size measurements, and skin, draining lymph node, spleen, and sera were investigated to check parasite load, spleen visceralization, cytokine expression, histopathological changes, and anti-Leishmania IgG levels. The lesion emergence time was inversely proportional to the parasite concentration in the inocula. Animals infected by 104 parasites presented nodular lesions, while those infected with 106 parasites often exhibited ulcerated lesions. The differences in the final lesion sizes were observed between 104 and 105 inocula or 104 and 106 inocula. High IFNG expression, anti-Leishmania IgG levels, and parasite load occurred independently of the inoculum used. A mild inflammatory skin involvement was observed in animals infected with 104 parasites, while extensive tissue damage and parasite spleen visceralization occurred with 105 and 106 parasites. These results indicate that inocula with different concentrations of parasites generate differences in the time of lesion emergence, clinical presentation, and systemic commitment, despite high and similar IFNG expression and parasite load. This suggests that a modulation in the immune response to different parasite numbers occurs in an early phase of the infection, which could dictate the establishment and magnitude of the chronic phase of the disease.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10191/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALraquel_romaoetal_IOC_2014.pdfapplication/pdf2595987https://www.arca.fiocruz.br/bitstream/icict/10191/2/raquel_romaoetal_IOC_2014.pdf3eaf96f6e5f8a4b3807ceddef223ff35MD52TEXTraquel_romaoetal_IOC_2014.pdf.txtraquel_romaoetal_IOC_2014.pdf.txtExtracted texttext/plain58143https://www.arca.fiocruz.br/bitstream/icict/10191/3/raquel_romaoetal_IOC_2014.pdf.txt561f4ed275fbe8e613a9a78704287ad9MD53icict/101912022-06-24 13:10:46.292oai:www.arca.fiocruz.br:icict/10191TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:10:46Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Comparative Evaluation of Lesion Development, Tissue Damage, and Cytokine Expression in Golden Hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrations
title Comparative Evaluation of Lesion Development, Tissue Damage, and Cytokine Expression in Golden Hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrations
spellingShingle Comparative Evaluation of Lesion Development, Tissue Damage, and Cytokine Expression in Golden Hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrations
Romão, Raquel P. Ribeiro
Leishmania
Mesocricetus
Leishmania
Golden Hamsters
Leishmania
Mesocricetus
Mesocricetus
Leishmania
title_short Comparative Evaluation of Lesion Development, Tissue Damage, and Cytokine Expression in Golden Hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrations
title_full Comparative Evaluation of Lesion Development, Tissue Damage, and Cytokine Expression in Golden Hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrations
title_fullStr Comparative Evaluation of Lesion Development, Tissue Damage, and Cytokine Expression in Golden Hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrations
title_full_unstemmed Comparative Evaluation of Lesion Development, Tissue Damage, and Cytokine Expression in Golden Hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrations
title_sort Comparative Evaluation of Lesion Development, Tissue Damage, and Cytokine Expression in Golden Hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrations
author Romão, Raquel P. Ribeiro
author_facet Romão, Raquel P. Ribeiro
Moreira, Otacilio C.
Osorio, Elvia Yaneth
Finkelstein, Lea Cysne
Silva, Adriano Gomes
Valverde, Joanna G
Pirmez, Claude
Cruz, Alda Maria da
Pinto, Eduardo Fonseca
author_role author
author2 Moreira, Otacilio C.
Osorio, Elvia Yaneth
Finkelstein, Lea Cysne
Silva, Adriano Gomes
Valverde, Joanna G
Pirmez, Claude
Cruz, Alda Maria da
Pinto, Eduardo Fonseca
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Romão, Raquel P. Ribeiro
Moreira, Otacilio C.
Osorio, Elvia Yaneth
Finkelstein, Lea Cysne
Silva, Adriano Gomes
Valverde, Joanna G
Pirmez, Claude
Cruz, Alda Maria da
Pinto, Eduardo Fonseca
dc.subject.other.pt_BR.fl_str_mv Leishmania
Mesocricetus
topic Leishmania
Mesocricetus
Leishmania
Golden Hamsters
Leishmania
Mesocricetus
Mesocricetus
Leishmania
dc.subject.en.pt_BR.fl_str_mv Leishmania
Golden Hamsters
dc.subject.es.pt_BR.fl_str_mv Leishmania
Mesocricetus
dc.subject.decs.pt_BR.fl_str_mv Mesocricetus
Leishmania
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-04T17:07:26Z
dc.date.available.fl_str_mv 2015-05-04T17:07:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ROMÃO, Raquel P. Ribeiro et al. Comparative evaluation of lesion developmente, tissue damage, and cytokine expression in golden hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrations. Infect. Immun., v.82, n.12, p.5203-5213, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10191
dc.identifier.issn.pt_BR.fl_str_mv 0019-9567
1098-5522
dc.identifier.doi.pt_BR.fl_str_mv 10.1128/IAI.02083-14
identifier_str_mv ROMÃO, Raquel P. Ribeiro et al. Comparative evaluation of lesion developmente, tissue damage, and cytokine expression in golden hamsters (Mesocricetus auratus) infected by Inocula with different Leishmania (Viannia) braziliensis concentrations. Infect. Immun., v.82, n.12, p.5203-5213, 2014.
0019-9567
1098-5522
10.1128/IAI.02083-14
url https://www.arca.fiocruz.br/handle/icict/10191
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/10191/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/10191/2/raquel_romaoetal_IOC_2014.pdf
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