Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs

Detalhes bibliográficos
Autor(a) principal: Pereira, Isabela Tiemy
Data de Publicação: 2018
Outros Autores: Spangenberg, Lucia, Robert, Anny Waloski, Amorín, Rocío, Stimamiglio, Marco Augusto, Naya, Hugo, Dallagiovanna, Bruno
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39880
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
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spelling Pereira, Isabela TiemySpangenberg, LuciaRobert, Anny WaloskiAmorín, RocíoStimamiglio, Marco AugustoNaya, HugoDallagiovanna, Bruno2020-02-11T18:06:05Z2020-02-11T18:06:05Z2018PEREIRA, Isabela Tiemy et al. Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs. Scientific Data, n. 5, p. 1-11, 2018.2052-4463https://www.arca.fiocruz.br/handle/icict/3988010.1038/sdata.2018.2872052-4463porNature ResearchPolysome profiling followed by RNA-seq of cardiac differentiation stages in hESCsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Institut Pasteur de Montevideo. Bioinformatics Unit. Mataojo, Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Institut Pasteur de Montevideo. Bioinformatics Unit. Mataojo, Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Institut Pasteur de Montevideo. Bioinformatics Unit. Mataojo, Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.The regulation of gene expression acts at numerous complementary levels to control and refine protein abundance. The analysis of mRNAs associated with polysomes, called polysome profiling, has been used to investigate the post-transcriptional mechanisms that are involved in different biological processes. Pluripotent stem cells are able to differentiate into a variety of cell lineages, and the cell commitment progression is carefully orchestrated. Genome-wide expression profiling has provided the possibility to investigate transcriptional changes during cardiomyogenesis; however, a more accurate study regarding post-transcriptional regulation is required. In the present work, we isolated and high-throughput sequenced ribosome-free and polysome-bound RNAs from NKX2-5eGFP/w HES3 undifferentiated pluripotent stem cells at the subsequent differentiation stages of cardiomyogenesis: embryoid body aggregation, mesoderm, cardiac progenitor and cardiomyocyte. The expression of developmental markers was followed by flow cytometry, and quality analyses were performed as technical controls to ensure high quality data. Our dataset provides valuable information about hESC cardiac differentiation and can be used to investigate genes potentially controlled by post-transcriptional mechanisms.PolyribosomesSequence Analysis, RNAEmbryonic Stem CellsPolirribosomasAnálisis de Secuencia de ARNCélulas Madre EmbrionariasPolirribossomosAnálise de Sequência de RNACélulas-Tronco Embrionáriasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/39880/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALsdata2018287ok.pdfsdata2018287ok.pdfapplication/pdf1686249https://www.arca.fiocruz.br/bitstream/icict/39880/2/sdata2018287ok.pdf1142b63f26643f8d521fa24335411163MD52TEXTsdata2018287ok.pdf.txtsdata2018287ok.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs
title Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs
spellingShingle Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs
Pereira, Isabela Tiemy
Polyribosomes
Sequence Analysis, RNA
Embryonic Stem Cells
Polirribosomas
Análisis de Secuencia de ARN
Células Madre Embrionarias
Polirribossomos
Análise de Sequência de RNA
Células-Tronco Embrionárias
title_short Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs
title_full Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs
title_fullStr Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs
title_full_unstemmed Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs
title_sort Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs
author Pereira, Isabela Tiemy
author_facet Pereira, Isabela Tiemy
Spangenberg, Lucia
Robert, Anny Waloski
Amorín, Rocío
Stimamiglio, Marco Augusto
Naya, Hugo
Dallagiovanna, Bruno
author_role author
author2 Spangenberg, Lucia
Robert, Anny Waloski
Amorín, Rocío
Stimamiglio, Marco Augusto
Naya, Hugo
Dallagiovanna, Bruno
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Isabela Tiemy
Spangenberg, Lucia
Robert, Anny Waloski
Amorín, Rocío
Stimamiglio, Marco Augusto
Naya, Hugo
Dallagiovanna, Bruno
dc.subject.en.pt_BR.fl_str_mv Polyribosomes
Sequence Analysis, RNA
Embryonic Stem Cells
topic Polyribosomes
Sequence Analysis, RNA
Embryonic Stem Cells
Polirribosomas
Análisis de Secuencia de ARN
Células Madre Embrionarias
Polirribossomos
Análise de Sequência de RNA
Células-Tronco Embrionárias
dc.subject.es.pt_BR.fl_str_mv Polirribosomas
Análisis de Secuencia de ARN
Células Madre Embrionarias
dc.subject.decs.pt_BR.fl_str_mv Polirribossomos
Análise de Sequência de RNA
Células-Tronco Embrionárias
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2020-02-11T18:06:05Z
dc.date.available.fl_str_mv 2020-02-11T18:06:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEREIRA, Isabela Tiemy et al. Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs. Scientific Data, n. 5, p. 1-11, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39880
dc.identifier.issn.pt_BR.fl_str_mv 2052-4463
dc.identifier.doi.none.fl_str_mv 10.1038/sdata.2018.287
dc.identifier.eissn.none.fl_str_mv 2052-4463
identifier_str_mv PEREIRA, Isabela Tiemy et al. Polysome profiling followed by RNA-seq of cardiac differentiation stages in hESCs. Scientific Data, n. 5, p. 1-11, 2018.
2052-4463
10.1038/sdata.2018.287
url https://www.arca.fiocruz.br/handle/icict/39880
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/39880/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/39880/2/sdata2018287ok.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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