Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance

Detalhes bibliográficos
Autor(a) principal: Marcon, Bruna Hilzendeger
Data de Publicação: 2019
Outros Autores: Shigunov, Patrícia, Spangenberg, Lucia, Pereira, Isabela Tiemy, Aguiar, Alessandra Melo de, Amorín, Rocío, Rebelatto, Carmen Lúcia Kuniyoshi, Dominguez, Alejandro Correa, Dallagiovanna, Bruno
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32891
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
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spelling Marcon, Bruna HilzendegerShigunov, PatríciaSpangenberg, LuciaPereira, Isabela TiemyAguiar, Alessandra Melo deAmorín, RocíoRebelatto, Carmen Lúcia KuniyoshiDominguez, Alejandro CorreaDallagiovanna, Bruno2019-04-30T18:19:40Z2019-04-30T18:19:40Z2019MARCON, Bruna H. et al. Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance. Scientific Reports, v. 9, n. 5611, p. 1-10, 2019.2045-2322https://www.arca.fiocruz.br/handle/icict/3289110.1038/s41598-019-42005-32045-2322porNature ResearchPerfil PolissômicoStem CellsAdipogenesisPolyribosomesRNA, MessengerDown-RegulationCélulas MadreAdipogénesisPolirribosomasARN MensajeroRegulación hacia AbajoCélulas-TroncoAdipogeniaPolirribossomosRNA MensageiroRegulação para BaixoCell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundanceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto Pasteur. Unidad de Bioinformática. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto Pasteur. Unidad de Bioinformática. Montevideo, Uruguay.Pontifícia Universidade Católica do Paraná. Núcleo de Tecnologia Celular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.The adipogenic process is characterized by the expression of adipocyte differentiation markers that lead to changes in cell metabolism and to the accumulation of lipid droplets. Moreover, during early adipogenesis, cells undergo a strong downregulation of translational activity with a decrease in cell size, proliferation and migration. In the present study, we identified that after 24 hours of adipogenic induction, human adipose tissue-derived stem cells (hASCs) undergo a G1-cell cycle arrest consistent with reduced proliferation, and this effect was correlated with a shift in polysome profile with an enrichment of the monosomal fraction and a reduction of the polysomal fraction. Polysome profiling analysis also revealed that this change in the monosomal/polysomal ratio was related to a strong downregulation of cell cycle and proliferation genes, such as cyclins and cyclin-dependent kinases (CDKs). Comparing total and polysome-associated mRNA sequencing, we also observed that this downregulation was mostly due to a reduction of cell cycle and proliferation transcripts via control of total mRNA abundance, rather than by translational control.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/32891/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALs41598-019-42005-3.pdfs41598-019-42005-3.pdfapplication/pdf2386137https://www.arca.fiocruz.br/bitstream/icict/32891/2/s41598-019-42005-3.pdfdccd189dd711688e0f6fcd0b24ad0c8cMD52TEXTs41598-019-42005-3.pdf.txts41598-019-42005-3.pdf.txtExtracted texttext/plain50031https://www.arca.fiocruz.br/bitstream/icict/32891/3/s41598-019-42005-3.pdf.txt75d19a9ffd5520b049b367c77da03860MD53icict/328912019-11-01 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dc.title.pt_BR.fl_str_mv Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance
title Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance
spellingShingle Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance
Marcon, Bruna Hilzendeger
Perfil Polissômico
Stem Cells
Adipogenesis
Polyribosomes
RNA, Messenger
Down-Regulation
Células Madre
Adipogénesis
Polirribosomas
ARN Mensajero
Regulación hacia Abajo
Células-Tronco
Adipogenia
Polirribossomos
RNA Mensageiro
Regulação para Baixo
title_short Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance
title_full Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance
title_fullStr Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance
title_full_unstemmed Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance
title_sort Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance
author Marcon, Bruna Hilzendeger
author_facet Marcon, Bruna Hilzendeger
Shigunov, Patrícia
Spangenberg, Lucia
Pereira, Isabela Tiemy
Aguiar, Alessandra Melo de
Amorín, Rocío
Rebelatto, Carmen Lúcia Kuniyoshi
Dominguez, Alejandro Correa
Dallagiovanna, Bruno
author_role author
author2 Shigunov, Patrícia
Spangenberg, Lucia
Pereira, Isabela Tiemy
Aguiar, Alessandra Melo de
Amorín, Rocío
Rebelatto, Carmen Lúcia Kuniyoshi
Dominguez, Alejandro Correa
Dallagiovanna, Bruno
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Marcon, Bruna Hilzendeger
Shigunov, Patrícia
Spangenberg, Lucia
Pereira, Isabela Tiemy
Aguiar, Alessandra Melo de
Amorín, Rocío
Rebelatto, Carmen Lúcia Kuniyoshi
Dominguez, Alejandro Correa
Dallagiovanna, Bruno
dc.subject.other.pt_BR.fl_str_mv Perfil Polissômico
topic Perfil Polissômico
Stem Cells
Adipogenesis
Polyribosomes
RNA, Messenger
Down-Regulation
Células Madre
Adipogénesis
Polirribosomas
ARN Mensajero
Regulación hacia Abajo
Células-Tronco
Adipogenia
Polirribossomos
RNA Mensageiro
Regulação para Baixo
dc.subject.en.pt_BR.fl_str_mv Stem Cells
Adipogenesis
Polyribosomes
RNA, Messenger
Down-Regulation
dc.subject.es.pt_BR.fl_str_mv Células Madre
Adipogénesis
Polirribosomas
ARN Mensajero
Regulación hacia Abajo
dc.subject.decs.pt_BR.fl_str_mv Células-Tronco
Adipogenia
Polirribossomos
RNA Mensageiro
Regulação para Baixo
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-04-30T18:19:40Z
dc.date.available.fl_str_mv 2019-04-30T18:19:40Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARCON, Bruna H. et al. Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance. Scientific Reports, v. 9, n. 5611, p. 1-10, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32891
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-019-42005-3
dc.identifier.eissn.none.fl_str_mv 2045-2322
identifier_str_mv MARCON, Bruna H. et al. Cell cycle genes are downregulated after adipogenic triggering in human adipose tissue-derived stem cells by regulation of mRNA abundance. Scientific Reports, v. 9, n. 5611, p. 1-10, 2019.
2045-2322
10.1038/s41598-019-42005-3
url https://www.arca.fiocruz.br/handle/icict/32891
dc.language.iso.fl_str_mv por
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/32891/2/s41598-019-42005-3.pdf
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