Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes

Detalhes bibliográficos
Autor(a) principal: Pereira, Isabela Tiemy
Data de Publicação: 2019
Outros Autores: Spangenberg, Lucia, Robert, Anny Waloski, Amorín, Rocío, Stimamiglio, Marco Augusto, Naya, Hugo, Dallagiovanna, Bruno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33134
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
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spelling Pereira, Isabela TiemySpangenberg, LuciaRobert, Anny WaloskiAmorín, RocíoStimamiglio, Marco AugustoNaya, HugoDallagiovanna, Bruno2019-05-17T19:42:36Z2019-05-17T19:42:36Z2019PEREIRA, Isabela Tiemy et al. Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes. BMC Genomics, v. 20, n. 219, p. 1-16, 2019.1471-2164https://www.arca.fiocruz.br/handle/icict/3313410.1186/s12864-019-5550-31471-2164engBMCCardiomogênesePerfil PolysomeCardiomyogenesisCardiomyocytesPolyribosomesGene Expression RegulationRNA Processing, Post-TranscriptionalMiocitos CardíacosPolirribosomasRegulación de la Expresión GénicaProcesamiento Postranscripcional del ARNMiócitos CardíacosPolirribossomosRegulação da Expressão GênicaProcessamento Pós-Transcricional do RNACardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Institut Pasteur. Bioinformatics Unit. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Institut Pasteur. Bioinformatics Unit. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Institut Pasteur. Bioinformatics Unit. Montevideo, Uruguay.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.Cardiac cell fate specification occurs through progressive steps, and its gene expression regulation features are still being defined. There has been an increasing interest in understanding the coordination between transcription and post-transcriptional regulation during the differentiation processes. Here, we took advantage of the polysome profiling technique to isolate and high-throughput sequence ribosome-free and polysome-bound RNAs during cardiomyogenesis. We showed that polysome-bound RNAs exhibit the cardiomyogenic commitment gene expression and that mesoderm-to-cardiac progenitor stages are strongly regulated. Additionally, we compared ribosome-free and polysome-bound RNAs and found that the post-transcriptional regulation vastly contributes to cardiac phenotype determination, including RNA recruitment to and dissociation from ribosomes. Moreover, we found that protein synthesis is decreased in cardiomyocytes compared to human embryonic stem-cells (hESCs), possibly due to the down-regulation of translation-related genes. Our data provided a powerful tool to investigate genes potentially controlled by post-transcriptional mechanisms during the cardiac differentiation of hESC. This work could prospect fundamental tools to develop new therapy and research approaches.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/33134/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALs12864-019-5550-3.pdfs12864-019-5550-3.pdfapplication/pdf6090125https://www.arca.fiocruz.br/bitstream/icict/33134/2/s12864-019-5550-3.pdfb4de3e138c915f8c5d4cb6f834e51631MD52TEXTs12864-019-5550-3.pdf.txts12864-019-5550-3.pdf.txtExtracted texttext/plain66584https://www.arca.fiocruz.br/bitstream/icict/33134/3/s12864-019-5550-3.pdf.txtdf8b815914ce41a7cdd177940af9c7aaMD53icict/331342019-09-12 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dc.title.pt_BR.fl_str_mv Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes
title Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes
spellingShingle Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes
Pereira, Isabela Tiemy
Cardiomogênese
Perfil Polysome
Cardiomyogenesis
Cardiomyocytes
Polyribosomes
Gene Expression Regulation
RNA Processing, Post-Transcriptional
Miocitos Cardíacos
Polirribosomas
Regulación de la Expresión Génica
Procesamiento Postranscripcional del ARN
Miócitos Cardíacos
Polirribossomos
Regulação da Expressão Gênica
Processamento Pós-Transcricional do RNA
title_short Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes
title_full Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes
title_fullStr Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes
title_full_unstemmed Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes
title_sort Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes
author Pereira, Isabela Tiemy
author_facet Pereira, Isabela Tiemy
Spangenberg, Lucia
Robert, Anny Waloski
Amorín, Rocío
Stimamiglio, Marco Augusto
Naya, Hugo
Dallagiovanna, Bruno
author_role author
author2 Spangenberg, Lucia
Robert, Anny Waloski
Amorín, Rocío
Stimamiglio, Marco Augusto
Naya, Hugo
Dallagiovanna, Bruno
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Isabela Tiemy
Spangenberg, Lucia
Robert, Anny Waloski
Amorín, Rocío
Stimamiglio, Marco Augusto
Naya, Hugo
Dallagiovanna, Bruno
dc.subject.other.pt_BR.fl_str_mv Cardiomogênese
Perfil Polysome
topic Cardiomogênese
Perfil Polysome
Cardiomyogenesis
Cardiomyocytes
Polyribosomes
Gene Expression Regulation
RNA Processing, Post-Transcriptional
Miocitos Cardíacos
Polirribosomas
Regulación de la Expresión Génica
Procesamiento Postranscripcional del ARN
Miócitos Cardíacos
Polirribossomos
Regulação da Expressão Gênica
Processamento Pós-Transcricional do RNA
dc.subject.en.pt_BR.fl_str_mv Cardiomyogenesis
Cardiomyocytes
Polyribosomes
Gene Expression Regulation
RNA Processing, Post-Transcriptional
dc.subject.es.pt_BR.fl_str_mv Miocitos Cardíacos
Polirribosomas
Regulación de la Expresión Génica
Procesamiento Postranscripcional del ARN
dc.subject.decs.pt_BR.fl_str_mv Miócitos Cardíacos
Polirribossomos
Regulação da Expressão Gênica
Processamento Pós-Transcricional do RNA
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-05-17T19:42:36Z
dc.date.available.fl_str_mv 2019-05-17T19:42:36Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEREIRA, Isabela Tiemy et al. Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes. BMC Genomics, v. 20, n. 219, p. 1-16, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33134
dc.identifier.issn.pt_BR.fl_str_mv 1471-2164
dc.identifier.doi.none.fl_str_mv 10.1186/s12864-019-5550-3
dc.identifier.eissn.none.fl_str_mv 1471-2164
identifier_str_mv PEREIRA, Isabela Tiemy et al. Cardiomyogenic differentiation is fine-tuned by differential mRNA association with polysomes. BMC Genomics, v. 20, n. 219, p. 1-16, 2019.
1471-2164
10.1186/s12864-019-5550-3
url https://www.arca.fiocruz.br/handle/icict/33134
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/33134/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/33134/2/s12864-019-5550-3.pdf
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