Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing Cells

Detalhes bibliográficos
Autor(a) principal: Póvoa, Tiago F.
Data de Publicação: 2016
Outros Autores: Oliveira, Edson R. A., Oliveira, Carlos A. Basílio de, Nuovo, Gerard J., Chagas, Vera .L .A., Salomão, Natália G., Mota, Ester M., Paes, Marciano V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1371/journal.pone.0168973
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18020
Resumo: Inclui correção. Dr. Ada Maria de Barcelos Alves should be included in the author byline instead of the Acknowledgments. She should be listed as the seventh author and affiliated with Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. The contributions of this author are as follows: Conceived and designed the experiments, performed the experiments, analyzed the data, and contributed reagents/materials/analysis tools.
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spelling Póvoa, Tiago F.Oliveira, Edson R. A.Oliveira, Carlos A. Basílio deNuovo, Gerard J.Chagas, Vera .L .A.Salomão, Natália G.Mota, Ester M.Paes, Marciano V.2017-03-13T15:47:41Z2017-03-13T15:47:41Z2016PÓVOA, Tiago F. et al. Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alphaand RANTES-Producing Cells. PLoS ONE, v. 11, n. 12, p. 1-19, Dec. 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1802010.1371/journal.pone.0168973Inclui correção. Dr. Ada Maria de Barcelos Alves should be included in the author byline instead of the Acknowledgments. She should be listed as the seventh author and affiliated with Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. The contributions of this author are as follows: Conceived and designed the experiments, performed the experiments, analyzed the data, and contributed reagents/materials/analysis tools.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia de Infecções Virais. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Laboratório de Modelagem Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gaffreé Guinle. Departamento de Anatomia Patológica. Rio de Janeiro, RJ, Brasil.Ohio State University Comprehensive Cancer Center. Columbus, Ohio, USA / Phylogeny In. Powell, Ohio, USA.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Anatomia Patológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Patologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ. Brasil.Dengue disease is an acute viral illness caused by dengue virus (DENV) that can progress to hemorrhagic stages leading to about 20000 deaths every year worldwide. Despite many clinical investigations regarding dengue, the immunopathogenic process by which infected patients evolve to the severe forms is not fully understood. Apart from differences in virulence and the antibody cross reactivity that can potentially augment virus replication, imbalanced cellular immunity is also seen as a major concern in the establishment of severe dengue. In this context, the investigation of cellular immunity and its products in dengue fatal cases may provide valuable data to help revealing dengue immunopathogenesis. Here, based in four dengue fatal cases infected by the serotype 3 in Brazil, different peripheral organs (livers, lungs and kidneys) were studied to evaluate the presence of cell infiltrates and the patterns of local cytokine response. The overall scenario of the studied cases revealed a considerable systemic involvement of infection with mononuclear cells targeted to all of the evaluated organs, as measured by immunohistochemistry (IHC). Quantification of cytokine-expressing cells in peripheral tissues was also performed to characterize the ongoing inflammatory process by the severe stage of the disease. Increased levels of IFN-γ- and TNF-α-expressing cells in liver, lung and kidney samples of post-mortem subjects evidenced a strong pro-inflammatory induction in these tissues. The presence of increased RANTES-producing cell numbers in all analyzed organs suggested a possible link between the clinical status and altered vascular permeability. Co-staining of DENV RNA and IFN-γ or TNF-α using in situ hibridization and IHC confirmed the virus-specific trigger of the pro-inflammatory response. Taken together, this work provided additional evidences that corroborated with the traditional theories regarding the "cytokine storm" and the occurrence of uneven cellular immunity in response to DENV as major reasons for progress to severe disease.engPublic Library of ScienceResposta Pró-InflamatóriaDengueCasos fataisFator de Necrose Tumoral alfaQuimiocina CCL5IFN-GammaTNF-AlphaRANTES-Producing CellsDengueFatal CasesPro-Inflammatory ResponsePeripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing Cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing Cells
title Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing Cells
spellingShingle Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing Cells
Póvoa, Tiago F.
Resposta Pró-Inflamatória
Dengue
Casos fatais
Fator de Necrose Tumoral alfa
Quimiocina CCL5
IFN-Gamma
TNF-Alpha
RANTES-Producing Cells
Dengue
Fatal Cases
Pro-Inflammatory Response
title_short Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing Cells
title_full Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing Cells
title_fullStr Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing Cells
title_full_unstemmed Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing Cells
title_sort Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alpha- and RANTES-Producing Cells
author Póvoa, Tiago F.
author_facet Póvoa, Tiago F.
Oliveira, Edson R. A.
Oliveira, Carlos A. Basílio de
Nuovo, Gerard J.
Chagas, Vera .L .A.
Salomão, Natália G.
Mota, Ester M.
Paes, Marciano V.
author_role author
author2 Oliveira, Edson R. A.
Oliveira, Carlos A. Basílio de
Nuovo, Gerard J.
Chagas, Vera .L .A.
Salomão, Natália G.
Mota, Ester M.
Paes, Marciano V.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Póvoa, Tiago F.
Oliveira, Edson R. A.
Oliveira, Carlos A. Basílio de
Nuovo, Gerard J.
Chagas, Vera .L .A.
Salomão, Natália G.
Mota, Ester M.
Paes, Marciano V.
dc.subject.other.pt_BR.fl_str_mv Resposta Pró-Inflamatória
Dengue
Casos fatais
Fator de Necrose Tumoral alfa
Quimiocina CCL5
topic Resposta Pró-Inflamatória
Dengue
Casos fatais
Fator de Necrose Tumoral alfa
Quimiocina CCL5
IFN-Gamma
TNF-Alpha
RANTES-Producing Cells
Dengue
Fatal Cases
Pro-Inflammatory Response
dc.subject.en.pt_BR.fl_str_mv IFN-Gamma
TNF-Alpha
RANTES-Producing Cells
Dengue
Fatal Cases
Pro-Inflammatory Response
description Inclui correção. Dr. Ada Maria de Barcelos Alves should be included in the author byline instead of the Acknowledgments. She should be listed as the seventh author and affiliated with Laboratory of Biotechnology and Physiology of Viral Infections, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil. The contributions of this author are as follows: Conceived and designed the experiments, performed the experiments, analyzed the data, and contributed reagents/materials/analysis tools.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-03-13T15:47:41Z
dc.date.available.fl_str_mv 2017-03-13T15:47:41Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PÓVOA, Tiago F. et al. Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alphaand RANTES-Producing Cells. PLoS ONE, v. 11, n. 12, p. 1-19, Dec. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18020
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0168973
identifier_str_mv PÓVOA, Tiago F. et al. Peripheral Organs of Dengue Fatal Cases Present Strong Pro-Inflammatory Response with Participation of IFN-Gamma-, TNF-Alphaand RANTES-Producing Cells. PLoS ONE, v. 11, n. 12, p. 1-19, Dec. 2016.
1932-6203
10.1371/journal.pone.0168973
url https://www.arca.fiocruz.br/handle/icict/18020
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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