Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test.

Detalhes bibliográficos
Autor(a) principal: Silva, Bosco Christiano Maciel da
Data de Publicação: 2014
Outros Autores: Grassi, Maria Fernanda Rios, Coutinho, Raimundo, Moreira, Rita Elizabeth, Mascarenhas, Viviana Nilla Olavarria, Borgo, Adriana Coutinho, Kalil, Jorge, Cunha Neto, Edecio, Fonseca, Simone Gonçalves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10450
Resumo: Instituto do Coração. Laboratório de Imunologia. São Paulo, SP, Brasil
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spelling Silva, Bosco Christiano Maciel daGrassi, Maria Fernanda RiosCoutinho, RaimundoMoreira, Rita ElizabethMascarenhas, Viviana Nilla OlavarriaBorgo, Adriana CoutinhoKalil, JorgeCunha Neto, EdecioFonseca, Simone Gonçalves2015-05-22T12:58:24Z2015-05-22T12:58:24Z2014SILVA, B. C. M et al. Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test. Memórias do Instituto Oswaldo Cruz, v. 109, n. 8, p. 999-1004, 2014.1678-8060https://www.arca.fiocruz.br/handle/icict/1045010.1590/0074-0276140193engInstituto Oswaldo Cruz. Ministério da SaúdeMycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto do Coração. Laboratório de Imunologia. São Paulo, SP, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilEscola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilInstituto do Coração. Laboratório de Imunologia. São Paulo, SP, Brasil / Universidade de São Paulo. Faculdade de Medicina. Departamento de Medicina. Divisão de Imunologia Clínica e Alergia. São Paulo, SP, BrasilInstituto do Coração. Laboratório de Imunologia. São Paulo, SP, Brasil / Universidade de São Paulo. Faculdade de Medicina. Departamento de Medicina. Divisão de Imunologia Clínica e Alergia. São Paulo, SP, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrasilInstituto do Coração. Laboratório de Imunologia. São Paulo, SP, Brasil / Universidade de São Paulo. Faculdade de Medicina. Departamento de Medicina. Divisão de Imunologia Clínica e Alergia. São Paulo, SP, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrasilInstituto do Coração. Laboratório de Imunologia. São Paulo, SP, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, BrasilThe interferon (IFN)-γ response to peptides can be a useful diagnostic marker of Mycobacterium tuberculosis (MTB) latent infection. We identified promiscuous and potentially protective CD4+ T-cell epitopes from the most conserved regions of MTB antigenic proteins by scanning the MTB antigenic proteins GroEL2, phosphate-binding protein 1 precursor and 19 kDa antigen with the TEPITOPE algorithm. Seven peptide sequences predicted to bind to multiple human leukocyte antigen (HLA)-DR molecules were synthesised and tested with IFN-γ enzyme-linked immunospot (ELISPOT) assays using peripheral blood mononuclear cells (PBMCs) from 16 Mantoux tuberculin skin test (TST)-positive and 16 TST-negative healthy donors. Eighty-eight percent of TST-positive donors responded to at least one of the peptides, compared to 25% of TST-negative donors. Each individual peptide induced IFN-γ production by PBMCs from at least 31% of the TST-positive donors. The magnitude of the response against all peptides was 182 ± 230 x 106 IFN-γ spot forming cells (SFC) among TST-positive donors and 36 ± 62 x 106 SFC among TST-negative donors (p = 0.007). The response to GroEL2 (463-477) was only observed in the TST-positive group. This combination of novel MTB CD4 T-cell epitopes should be tested in a larger cohort of individuals with latent tuberculosis (TB) to evaluate its potential to diagnose latent TB and it may be included in ELISPOT-based IFN-γ assays to identify individuals with this condition.Mycobacterium tuberculosisEpitopesIFN-γ ELISPOTLatent tuberculosis - Mantoux tuberculin skin testinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSilva BCM Mycobacterium....pdfSilva BCM Mycobacterium....pdfapplication/pdf447732https://www.arca.fiocruz.br/bitstream/icict/10450/2/Silva%20BCM%20Mycobacterium....pdff7089e0e13ea661aa984ec8a528e1f5aMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/10450/3/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD53TEXTSilva BCM Mycobacterium....pdf.txtSilva BCM Mycobacterium....pdf.txtExtracted texttext/plain34523https://www.arca.fiocruz.br/bitstream/icict/10450/4/Silva%20BCM%20Mycobacterium....pdf.txtf16362219903c60eea8a9db10caa997aMD54icict/104502023-03-15 14:33:25.126oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:25Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test.
title Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test.
spellingShingle Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test.
Silva, Bosco Christiano Maciel da
Mycobacterium tuberculosis
Epitopes
IFN-γ ELISPOT
Latent tuberculosis - Mantoux tuberculin skin test
title_short Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test.
title_full Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test.
title_fullStr Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test.
title_full_unstemmed Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test.
title_sort Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test.
author Silva, Bosco Christiano Maciel da
author_facet Silva, Bosco Christiano Maciel da
Grassi, Maria Fernanda Rios
Coutinho, Raimundo
Moreira, Rita Elizabeth
Mascarenhas, Viviana Nilla Olavarria
Borgo, Adriana Coutinho
Kalil, Jorge
Cunha Neto, Edecio
Fonseca, Simone Gonçalves
author_role author
author2 Grassi, Maria Fernanda Rios
Coutinho, Raimundo
Moreira, Rita Elizabeth
Mascarenhas, Viviana Nilla Olavarria
Borgo, Adriana Coutinho
Kalil, Jorge
Cunha Neto, Edecio
Fonseca, Simone Gonçalves
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Bosco Christiano Maciel da
Grassi, Maria Fernanda Rios
Coutinho, Raimundo
Moreira, Rita Elizabeth
Mascarenhas, Viviana Nilla Olavarria
Borgo, Adriana Coutinho
Kalil, Jorge
Cunha Neto, Edecio
Fonseca, Simone Gonçalves
dc.subject.en.pt_BR.fl_str_mv Mycobacterium tuberculosis
Epitopes
IFN-γ ELISPOT
Latent tuberculosis - Mantoux tuberculin skin test
topic Mycobacterium tuberculosis
Epitopes
IFN-γ ELISPOT
Latent tuberculosis - Mantoux tuberculin skin test
description Instituto do Coração. Laboratório de Imunologia. São Paulo, SP, Brasil
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-22T12:58:24Z
dc.date.available.fl_str_mv 2015-05-22T12:58:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, B. C. M et al. Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test. Memórias do Instituto Oswaldo Cruz, v. 109, n. 8, p. 999-1004, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10450
dc.identifier.issn.none.fl_str_mv 1678-8060
dc.identifier.doi.none.fl_str_mv 10.1590/0074-0276140193
identifier_str_mv SILVA, B. C. M et al. Mycobacterium tuberculosis epitope-specific interferon-g production in healthy Brazilians reactive and non-reactive to tuberculin skin test. Memórias do Instituto Oswaldo Cruz, v. 109, n. 8, p. 999-1004, 2014.
1678-8060
10.1590/0074-0276140193
url https://www.arca.fiocruz.br/handle/icict/10450
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz. Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz. Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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