Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects

Detalhes bibliográficos
Autor(a) principal: Moreira, Douglas de Souza
Data de Publicação: 2018
Outros Autores: Xavier, Mariana Vieira, Murta, Silvane Maria Fonseca
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33856
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.
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spelling Moreira, Douglas de SouzaXavier, Mariana VieiraMurta, Silvane Maria Fonseca2019-07-04T17:39:57Z2019-07-04T17:39:57Z2018MOREIRA, Douglas de Souza; XAVIER, Mariana Vieira; MURTA, Silvane Maria Fonseca. Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 12, p. 1-5, 2018.0074-0276https://www.arca.fiocruz.br/handle/icict/3385610.1590/0074-02760180377engFundação Oswaldo CruzLeishmania braziliensisPeroxidase de ascorbatoResistência antimônioDefesa oxidativaLeishmania braziliensisAscorbate peroxidaseAntimony resistanceOxidative defenceAscorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effectsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Ascorbate peroxidase (APX) is a redox enzyme of the trypanothione pathway that converts hydrogen peroxide (H2O2) into water molecules. In the present study, the APX gene was overexpressed in Leishmania braziliensis to investigate its contribution to the trivalent antimony (SbIII)-resistance phenotype. Western blot results demonstrated that APX-overexpressing parasites had higher APX protein levels in comparison with the wild-type line (LbWTS). APX-overexpressing clones showed an 8-fold increase in the antimony-resistance index over the parental line. In addition, our results indicated that these clones were approximately 1.8-fold more tolerant to H2O2 than the LbWTS line, suggesting that the APX enzyme plays an important role in the defence against oxidative stress. Susceptibility tests revealed that APX-overexpressing L. braziliensis lines were more resistant to isoniazid, an antibacterial agent that interacts with APX. Interestingly, this compound enhanced the anti-leishmanial SbIII effect, indicating that this combination represents a good strategy for leishmaniasis chemotherapy. Our data demonstrate that APX enzyme is involved in the development of L. braziliensisantimony-resistance phenotype and may be an attractive therapeutic target in the design of new strategies for leishmaniasis treatment.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/33856/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALAscorbate peroxidase overexpression protects.pdfAscorbate peroxidase overexpression protects.pdfapplication/pdf492382https://www.arca.fiocruz.br/bitstream/icict/33856/2/Ascorbate%20peroxidase%20overexpression%20protects.pdf17372f851baca54c1e74255751281366MD52TEXTAscorbate peroxidase overexpression protects.pdf.txtAscorbate peroxidase overexpression protects.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects
title Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects
spellingShingle Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects
Moreira, Douglas de Souza
Leishmania braziliensis
Peroxidase de ascorbato
Resistência antimônio
Defesa oxidativa
Leishmania braziliensis
Ascorbate peroxidase
Antimony resistance
Oxidative defence
title_short Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects
title_full Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects
title_fullStr Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects
title_full_unstemmed Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects
title_sort Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects
author Moreira, Douglas de Souza
author_facet Moreira, Douglas de Souza
Xavier, Mariana Vieira
Murta, Silvane Maria Fonseca
author_role author
author2 Xavier, Mariana Vieira
Murta, Silvane Maria Fonseca
author2_role author
author
dc.contributor.author.fl_str_mv Moreira, Douglas de Souza
Xavier, Mariana Vieira
Murta, Silvane Maria Fonseca
dc.subject.other.pt_BR.fl_str_mv Leishmania braziliensis
Peroxidase de ascorbato
Resistência antimônio
Defesa oxidativa
topic Leishmania braziliensis
Peroxidase de ascorbato
Resistência antimônio
Defesa oxidativa
Leishmania braziliensis
Ascorbate peroxidase
Antimony resistance
Oxidative defence
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
Ascorbate peroxidase
Antimony resistance
Oxidative defence
description Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-07-04T17:39:57Z
dc.date.available.fl_str_mv 2019-07-04T17:39:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOREIRA, Douglas de Souza; XAVIER, Mariana Vieira; MURTA, Silvane Maria Fonseca. Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 12, p. 1-5, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33856
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760180377
identifier_str_mv MOREIRA, Douglas de Souza; XAVIER, Mariana Vieira; MURTA, Silvane Maria Fonseca. Ascorbate peroxidase overexpression protects Leishmania braziliensis against trivalent antimony effects. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 113, n. 12, p. 1-5, 2018.
0074-0276
10.1590/0074-02760180377
url https://www.arca.fiocruz.br/handle/icict/33856
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
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