Colonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10227 |
Resumo: | Lancaster University. Faculty of Health and Medicine. Division of Biomedical and Life Sciences. Lancaster, UK / Universidade Federal de Minas Gerais, Instituto de Ciências Biomédicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. |
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Sant`Anna, Maurício R. V.Diaz-Albiter, HectorMartins, Kelsilândia AguiarAl Salem, Waleed S.Cavalcante, Reginald R.Dillon, ViV M.Bates, Paul A.Genta, Fernando A.Dillon, Rod J.2015-05-04T17:07:34Z2015-05-04T17:07:34Z2014SANT`ANNA, Maurício R. V. et al. Colonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infection. Parasites & Vectors, v. 7, n. 329, p. 1-10, 2014.https://www.arca.fiocruz.br/handle/icict/1022710.1186/1756-3305-7-329engBioMed CentralLeishmaniaLeishmaniaLeishmaniaAsaiaPseudozymaSerratiaSand lfyMicrobiotaLeishmaniaCiências BiológicasColonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLancaster University. Faculty of Health and Medicine. Division of Biomedical and Life Sciences. Lancaster, UK / Universidade Federal de Minas Gerais, Instituto de Ciências Biomédicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biomédicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Liverpool School of Tropical Medicine. Vector Group. Liverpool, UK.Universidade Federal do Piauí. Centro de Ciências da Saúde . Parasitologia e Microbiologia. Teresina, PI, Brasil.University of Liverpool. Institute of Integrative Biology. Liverpool. UK.Lancaster University. Faculty of Health and Medicine. Division of Biomedical and Life Sciences. Lancaster, UK .Lancaster University. Faculty of Health and Medicine. Division of Biomedical and Life Sciences. Lancaster, UK.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Lancaster University. Faculty of Health and Medicine. Division of Biomedical and Life Sciences. Lancaster, UK.Background: Phlebotomine sand flies transmit the haemoflagellate Leishmania, the causative agent of human leishmaniasis. The Leishmania promastigotes are confined to the gut lumen and are exposed to the gut microbiota within female sand flies. Here we study the colonisation resistance of yeast and bacteria in preventing the establishment of a Leishmania population in sand flies and the ability of Leishmania to provide colonisation resistance towards the insect bacterial pathogen Serratia marcescens that is also pathogenic towards Leishmania. Methods: We isolated microorganisms from wild-caught and laboratory-reared female Lutzomyia longipalpis, identified as Pseudozyma sp. Asaia sp. and Ochrobactrum intermedium. We fed the females with a sugar meal containing the microorganisms and then subsequently fed them with a bloodmeal containing Leishmania mexicana and recorded the development of the Leishmania population. Further experiments examined the effect of first colonising the sand fly gut with L. mexicana followed by feeding with, Serratia marcescens, an insect bacterial pathogen. The mortality of the flies due to S. marcescens was recorded in the presence and absence of Leishmania. Results: There was a reduction in the number of flies harbouring a Leishmania population that had been pre-fed with Pseudozyma sp. and Asaia sp. or O. intermedium. Experiments in which L. mexicana colonised the sand fly gut prior to being fed an insect bacterial pathogen, Serratia marcescens, showed that the survival of flies with a Leishmania infection was significantly higher compared to flies without Leishmania infection. Conclusions: The yeast and bacterial colonisation experiments show that the presence of sand fly gut microorganisms reduce the potential for Leishmania to establish within the sand fly vector. Sand flies infected with Leishmania were able to survive an attack by the bacterial pathogen that would have killed the insect and we concluded that Leishmania may benefit its insect host whilst increasing the potential to establish itself in the sand fly vector. We suggest that the increased ability of the sand fly to withstand a bacterial entomopathogen, due to the presence of the Leishmania, may provide an evolutionary pressure for the maintenance of the Leishmania-vector association.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10227/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALhector_albiteretal_IOC_2014.pdfapplication/pdf795343https://www.arca.fiocruz.br/bitstream/icict/10227/2/hector_albiteretal_IOC_2014.pdfb7962bd45a502eb13417f6eb5d41ab7aMD52TEXThector_albiteretal_IOC_2014.pdf.txthector_albiteretal_IOC_2014.pdf.txtExtracted texttext/plain47857https://www.arca.fiocruz.br/bitstream/icict/10227/3/hector_albiteretal_IOC_2014.pdf.txt35f850d1d26f404284d776672182ab33MD53icict/102272022-06-24 13:10:48.946oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:10:48Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Colonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infection |
| title |
Colonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infection |
| spellingShingle |
Colonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infection Sant`Anna, Maurício R. V. Leishmania Leishmania Leishmania Asaia Pseudozyma Serratia Sand lfy Microbiota Leishmania Ciências Biológicas |
| title_short |
Colonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infection |
| title_full |
Colonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infection |
| title_fullStr |
Colonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infection |
| title_full_unstemmed |
Colonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infection |
| title_sort |
Colonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infection |
| author |
Sant`Anna, Maurício R. V. |
| author_facet |
Sant`Anna, Maurício R. V. Diaz-Albiter, Hector Martins, Kelsilândia Aguiar Al Salem, Waleed S. Cavalcante, Reginald R. Dillon, ViV M. Bates, Paul A. Genta, Fernando A. Dillon, Rod J. |
| author_role |
author |
| author2 |
Diaz-Albiter, Hector Martins, Kelsilândia Aguiar Al Salem, Waleed S. Cavalcante, Reginald R. Dillon, ViV M. Bates, Paul A. Genta, Fernando A. Dillon, Rod J. |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Sant`Anna, Maurício R. V. Diaz-Albiter, Hector Martins, Kelsilândia Aguiar Al Salem, Waleed S. Cavalcante, Reginald R. Dillon, ViV M. Bates, Paul A. Genta, Fernando A. Dillon, Rod J. |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania |
| topic |
Leishmania Leishmania Leishmania Asaia Pseudozyma Serratia Sand lfy Microbiota Leishmania Ciências Biológicas |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania Leishmania Asaia Pseudozyma Serratia Sand lfy Microbiota |
| dc.subject.es.pt_BR.fl_str_mv |
Leishmania |
| dc.subject.decs.pt_BR.fl_str_mv |
Ciências Biológicas |
| description |
Lancaster University. Faculty of Health and Medicine. Division of Biomedical and Life Sciences. Lancaster, UK / Universidade Federal de Minas Gerais, Instituto de Ciências Biomédicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. |
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2014 |
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2014 |
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2015-05-04T17:07:34Z |
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2015-05-04T17:07:34Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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SANT`ANNA, Maurício R. V. et al. Colonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infection. Parasites & Vectors, v. 7, n. 329, p. 1-10, 2014. |
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https://www.arca.fiocruz.br/handle/icict/10227 |
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10.1186/1756-3305-7-329 |
| identifier_str_mv |
SANT`ANNA, Maurício R. V. et al. Colonisation resistance in the sand fly gut: Leishmania protects Lutzomyia longipalpis from bacterial infection. Parasites & Vectors, v. 7, n. 329, p. 1-10, 2014. 10.1186/1756-3305-7-329 |
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BioMed Central |
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BioMed Central |
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