Characterization of Leptospira isolates from humans and the environment in Uruguay
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/24788 |
Resumo: | Universidad de la República. Uruguay. |
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Meny, PaulinaMenéndez, ClaraQuintero, JairHernández, ElbaRíos, CristinaBalassiano, Ilana TeruszkinTrindade, Camilla Nunes dos ReisVital Brazil, Juliana MagalhãesRamos, Tatiane Mendes VarelaAshfield, NataliaFeble, CamilaAvila, EsthefaniSchelotto, FelipeVarela, Gustavo2018-02-09T14:02:04Z2018-02-09T14:02:04Z2017MENY, Paulina; et al. Characterization of Leptospira isolates from humans and the environment in Uruguay. Rev Inst Med Trop São Paulo, v.59: e79, 9p, 2017.0036-4665https://www.arca.fiocruz.br/handle/icict/2478810.1590/S1678-99462017590791678-9946engUniversidade de São PauloLeptospiroseHumanosUruguaiLeptospiraHuman isolatesMLVA-VNTR-PCRLeptospirosisEnvironment LeptospiraLeptospira interrogansLeptospira kirschneriL. meyeriUruguayCharacterization of Leptospira isolates from humans and the environment in Uruguayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidad de la República. Uruguay.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Centro de Referência Nacional para Leptospirose. WHO Collaborating Center for Leptospirosis. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Centro de Referência Nacional para Leptospirose. WHO Collaborating Center for Leptospirosis. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Centro de Referência Nacional para Leptospirose. WHO Collaborating Center for Leptospirosis. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas, Centro de Referência Nacional para Leptospirose. WHO Collaborating Center for Leptospirosis. Rio de Janeiro, RJ, Brasil.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Universidad de la República. Uruguay.Laboratory diagnosis of human leptospirosis usually relies on indirect methods exploring specific immune response. Isolation and identification of the involved strains are cumbersome, but can provide biological resources for pathogenic studies and relevant information for guiding prevention and control measures. The aim of the research we are hereby reporting was the characterization of Leptospira isolates obtained from humans and the environment in Uruguay. Blood cultures were performed from early samples of 302 Uruguayan patients, mainly rural workers, and from 36 water samples taken from their living or working environments. Eight human isolates and seven environmental isolates were obtained and analyzed by end point Polymerase Chain Reaction (PCR), Multilocus Variable Number of Tandem Repeat Analysis (MLVA) and other molecular methods. Human isolates corresponded to several serogroups and serovars of Leptospira interrogans and Leptospira kirschneri species, probably reflecting the infection with similar involved Leptospira species and serovars of an extended animal reservoir in rural settings of the country, mostly dedicated to meat and dairy production. Culture-positive patients were older than usually affected workers, and presented signs and symptoms of severe illness. A high organic and circulating bacterial burden may explain an easier positive result from these workers' samples. Environmental isolates were mainly identified as Leptospira biflexa strains, with a single L. meyeri isolate of uncertain significance.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24788/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALilana_balassiano_etal_IOC_2017.pdfilana_balassiano_etal_IOC_2017.pdfapplication/pdf363896https://www.arca.fiocruz.br/bitstream/icict/24788/2/ilana_balassiano_etal_IOC_2017.pdf7d4f35dfa3e09d7c09bf4c73cbb5dccfMD52TEXTilana_balassiano_etal_IOC_2017.pdf.txtilana_balassiano_etal_IOC_2017.pdf.txtExtracted texttext/plain40194https://www.arca.fiocruz.br/bitstream/icict/24788/3/ilana_balassiano_etal_IOC_2017.pdf.txt4dfdf6399c5ac1dbc8584655382f0ac9MD53icict/247882018-08-15 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| dc.title.pt_BR.fl_str_mv |
Characterization of Leptospira isolates from humans and the environment in Uruguay |
| title |
Characterization of Leptospira isolates from humans and the environment in Uruguay |
| spellingShingle |
Characterization of Leptospira isolates from humans and the environment in Uruguay Meny, Paulina Leptospirose Humanos Uruguai Leptospira Human isolates MLVA-VNTR-PCR Leptospirosis Environment Leptospira Leptospira interrogans Leptospira kirschneri L. meyeri Uruguay |
| title_short |
Characterization of Leptospira isolates from humans and the environment in Uruguay |
| title_full |
Characterization of Leptospira isolates from humans and the environment in Uruguay |
| title_fullStr |
Characterization of Leptospira isolates from humans and the environment in Uruguay |
| title_full_unstemmed |
Characterization of Leptospira isolates from humans and the environment in Uruguay |
| title_sort |
Characterization of Leptospira isolates from humans and the environment in Uruguay |
| author |
Meny, Paulina |
| author_facet |
Meny, Paulina Menéndez, Clara Quintero, Jair Hernández, Elba Ríos, Cristina Balassiano, Ilana Teruszkin Trindade, Camilla Nunes dos Reis Vital Brazil, Juliana Magalhães Ramos, Tatiane Mendes Varela Ashfield, Natalia Feble, Camila Avila, Esthefani Schelotto, Felipe Varela, Gustavo |
| author_role |
author |
| author2 |
Menéndez, Clara Quintero, Jair Hernández, Elba Ríos, Cristina Balassiano, Ilana Teruszkin Trindade, Camilla Nunes dos Reis Vital Brazil, Juliana Magalhães Ramos, Tatiane Mendes Varela Ashfield, Natalia Feble, Camila Avila, Esthefani Schelotto, Felipe Varela, Gustavo |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Meny, Paulina Menéndez, Clara Quintero, Jair Hernández, Elba Ríos, Cristina Balassiano, Ilana Teruszkin Trindade, Camilla Nunes dos Reis Vital Brazil, Juliana Magalhães Ramos, Tatiane Mendes Varela Ashfield, Natalia Feble, Camila Avila, Esthefani Schelotto, Felipe Varela, Gustavo |
| dc.subject.other.pt_BR.fl_str_mv |
Leptospirose Humanos Uruguai |
| topic |
Leptospirose Humanos Uruguai Leptospira Human isolates MLVA-VNTR-PCR Leptospirosis Environment Leptospira Leptospira interrogans Leptospira kirschneri L. meyeri Uruguay |
| dc.subject.en.pt_BR.fl_str_mv |
Leptospira Human isolates MLVA-VNTR-PCR Leptospirosis Environment Leptospira Leptospira interrogans Leptospira kirschneri L. meyeri Uruguay |
| description |
Universidad de la República. Uruguay. |
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2017 |
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2017 |
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2018-02-09T14:02:04Z |
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2018-02-09T14:02:04Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MENY, Paulina; et al. Characterization of Leptospira isolates from humans and the environment in Uruguay. Rev Inst Med Trop São Paulo, v.59: e79, 9p, 2017. |
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https://www.arca.fiocruz.br/handle/icict/24788 |
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0036-4665 |
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10.1590/S1678-9946201759079 |
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1678-9946 |
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MENY, Paulina; et al. Characterization of Leptospira isolates from humans and the environment in Uruguay. Rev Inst Med Trop São Paulo, v.59: e79, 9p, 2017. 0036-4665 10.1590/S1678-9946201759079 1678-9946 |
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Universidade de São Paulo |
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Universidade de São Paulo |
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