Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/56800 |
Resumo: | Universidade Federal de Minas Gerais. Centro de Tecnologia de Vacinas. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil. |
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Bagno, Flávia FonsecaSérgio, Sarah Aparecida RodriguesFigueiredo, Maria MartaGodoi, Lara CarvalhoAndrade, Luis Adan FloresCastro, Natália Salazar deSoares, Camila PereiraAguiar, AndressaAlmeida, Flávia JaquelineSilva, Edimilson D daFerreira, Antônio G PDurigon, Edison LuizGazzinelli, Ricardo TostesTeixeira, Santuza Maria RibeiroFernandes, Ana Paula Salles MouraFonseca, Flavio Guimarães da2023-02-01T13:35:25Z2023-02-01T13:35:25Z2022BAGNO, Flávia Fonseca et al. Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19. J Clin Virol Plus, v. 2, n. 3, 100101, 2022. doi: 10.1016/j.jcvp.2022.100101.2667-0380https://www.arca.fiocruz.br/handle/icict/56800engElsevier Ltd.Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Centro de Tecnologia de Vacinas. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.Universidade Federal de Minas Gerais. Centro de Tecnologia de Vacinas. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Centro de Tecnologia de Vacinas. Belo Horizonte, MG, Brazil/Universidade Estadual de Minas Gerais. Divinópolis, MG, Brazil.Universidade Federal de Minas Gerais. Centro de Tecnologia de Vacinas. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Centro de Tecnologia de Vacinas. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Centro de Tecnologia de Vacinas. Belo Horizonte, MG, BrazilUniversidade de São Paulo. Instituto de Ciências Biomédicas. São Paulo, SP, Brazil.Geriatric and Convalescent Hospital Dom Pedro II. São Paulo, SP, Brazil.Department of Pediatrics. Santa Casa de São Paulo School of Medical Sciences. São Paulo, SP, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil.Universidade de São Paulo. Instituto de Ciências Biomédicas. São Paulo, SP, Brazil.Universidade Federal de Minas Gerais. Centro de Tecnologia de Vacinas. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Centro de Tecnologia de Vacinas. Belo Horizonte, MG, Brazil/ Universidade Federal de Minas Gerais, Depto de Bioquímica e Imunologia, Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Centro de Tecnologia de Vacinas. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Faculdade de Farmácia. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Centro de Tecnologia de Vacinas. Belo Horizonte, MG, Brasil/Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil.There is a massive demand to identify alternative methods to detect new cases of COVID-19 as well as to investigate the epidemiology of the disease. In many countries, importation of commercial kits poses a significant impact on their testing capacity and increases the costs for the public health system. We have developed an ELISA to detect IgG antibodies against SARS-CoV-2 using a recombinant viral nucleocapsid (rN) protein expressed in E. coli. Using a total of 894 clinical samples we showed that the rN-ELISA was able to detect IgG antibodies against SARS-CoV-2 with high sensitivity (97.5%) and specificity (96.3%) when compared to a commercial antibody test. After three external validation studies, we showed that the test accuracy was higher than 90%. The rN-ELISA IgG kit constitutes a convenient and specific method for the large-scale determination of SARS-CoV-2 antibodies in human sera with high reliability.COVID-19DiagnosisELISAPrototypingSARS-CoV-2Serological assayinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALDevelopment and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19.pdfDevelopment and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19.pdfapplication/pdf929531https://www.arca.fiocruz.br/bitstream/icict/56800/2/Development%20and%20validation%20of%20an%20enzyme-linked%20immunoassay%20kit%20for%20diagnosis%20and%20surveillance%20of%20COVID-19.pdfa0a54948cb2eed01c3bf5090613a7402MD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56800/1/license.txt5a560609d32a3863062d77ff32785d58MD51icict/568002023-02-01 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| dc.title.en_US.fl_str_mv |
Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19 |
| title |
Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19 |
| spellingShingle |
Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19 Bagno, Flávia Fonseca COVID-19 Diagnosis ELISA Prototyping SARS-CoV-2 Serological assay |
| title_short |
Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19 |
| title_full |
Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19 |
| title_fullStr |
Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19 |
| title_full_unstemmed |
Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19 |
| title_sort |
Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19 |
| author |
Bagno, Flávia Fonseca |
| author_facet |
Bagno, Flávia Fonseca Sérgio, Sarah Aparecida Rodrigues Figueiredo, Maria Marta Godoi, Lara Carvalho Andrade, Luis Adan Flores Castro, Natália Salazar de Soares, Camila Pereira Aguiar, Andressa Almeida, Flávia Jaqueline Silva, Edimilson D da Ferreira, Antônio G P Durigon, Edison Luiz Gazzinelli, Ricardo Tostes Teixeira, Santuza Maria Ribeiro Fernandes, Ana Paula Salles Moura Fonseca, Flavio Guimarães da |
| author_role |
author |
| author2 |
Sérgio, Sarah Aparecida Rodrigues Figueiredo, Maria Marta Godoi, Lara Carvalho Andrade, Luis Adan Flores Castro, Natália Salazar de Soares, Camila Pereira Aguiar, Andressa Almeida, Flávia Jaqueline Silva, Edimilson D da Ferreira, Antônio G P Durigon, Edison Luiz Gazzinelli, Ricardo Tostes Teixeira, Santuza Maria Ribeiro Fernandes, Ana Paula Salles Moura Fonseca, Flavio Guimarães da |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Bagno, Flávia Fonseca Sérgio, Sarah Aparecida Rodrigues Figueiredo, Maria Marta Godoi, Lara Carvalho Andrade, Luis Adan Flores Castro, Natália Salazar de Soares, Camila Pereira Aguiar, Andressa Almeida, Flávia Jaqueline Silva, Edimilson D da Ferreira, Antônio G P Durigon, Edison Luiz Gazzinelli, Ricardo Tostes Teixeira, Santuza Maria Ribeiro Fernandes, Ana Paula Salles Moura Fonseca, Flavio Guimarães da |
| dc.subject.en.en_US.fl_str_mv |
COVID-19 Diagnosis ELISA Prototyping SARS-CoV-2 Serological assay |
| topic |
COVID-19 Diagnosis ELISA Prototyping SARS-CoV-2 Serological assay |
| description |
Universidade Federal de Minas Gerais. Centro de Tecnologia de Vacinas. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brazil. |
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2022 |
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2022 |
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2023-02-01T13:35:25Z |
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2023-02-01T13:35:25Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
BAGNO, Flávia Fonseca et al. Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19. J Clin Virol Plus, v. 2, n. 3, 100101, 2022. doi: 10.1016/j.jcvp.2022.100101. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/56800 |
| dc.identifier.issn.en_US.fl_str_mv |
2667-0380 |
| identifier_str_mv |
BAGNO, Flávia Fonseca et al. Development and validation of an enzyme-linked immunoassay kit for diagnosis and surveillance of COVID-19. J Clin Virol Plus, v. 2, n. 3, 100101, 2022. doi: 10.1016/j.jcvp.2022.100101. 2667-0380 |
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https://www.arca.fiocruz.br/handle/icict/56800 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Elsevier Ltd. |
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Elsevier Ltd. |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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