First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: McCarthy, Christina B.
Data de Publicação: 2013
Outros Autores: Santini, María Soledad, Pimenta, Paulo Filemon Paolucci, Diambra, Luis A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30677
Resumo: Centro Regional de Estudios Genómicos. Facultad de Ciencias Exactas. Universidad Nacional de La Plata. Florencio Varela, Buenos Aires. Argentina/Departamento de Informática y Tecnología. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Pergamino, Buenos Aires, Argentina
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spelling McCarthy, Christina B.Santini, María SoledadPimenta, Paulo Filemon PaolucciDiambra, Luis A.2018-12-18T17:16:20Z2018-12-18T17:16:20Z2013MCCARTHY, Christina B. et al. First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis. PLoS One.,v. 8, n. 3, e58645, 20131932-6203https://www.arca.fiocruz.br/handle/icict/3067710.1371/journal.pone.0058645engPublic Library of ScienceLeishmaniasisVisceral leishmaniasisLeishmaniasisVisceral leishmaniasisFirst comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCentro Regional de Estudios Genómicos. Facultad de Ciencias Exactas. Universidad Nacional de La Plata. Florencio Varela, Buenos Aires. Argentina/Departamento de Informática y Tecnología. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Pergamino, Buenos Aires, ArgentinaCentro Nacional de Diagnóstico e Investigación en Endemoepidemias. Administración Nacional de Laboratorios e Institutos de Salud. Ministerio de Salud, Buenos Aires, Argentina.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratorio de Entomologia Medica. Belo Horizonte, MG, BrazilCentro Regional de Estudios Genómicos. Facultad de Ciencias Exactas. Universidad Nacional de La Plata. Florencio Varela, Buenos Aires. ArgentinaLeishmaniasis is a vector-borne disease with a complex epidemiology and ecology. Visceral leishmaniasis (VL) is its most severe clinical form as it results in death if not treated. In Latin America VL is caused by the protist parasite Leishmania infantum (syn. chagasi) and transmitted by Lutzomyia longipalpis. This phlebotomine sand fly is only found in the New World, from Mexico to Argentina. However, due to deforestation, migration and urbanisation, among others, VL in Latin America is undergoing an evident geographic expansion as well as dramatic changes in its transmission patterns. In this context, the first VL outbreak was recently reported in Argentina, which has already caused 7 deaths and 83 reported cases. Insect vector transcriptomic analyses enable the identification of molecules involved in the insect's biology and vector-parasite interaction. Previous studies on laboratory reared Lu. longipalpis have provided a descriptive repertoire of gene expression in the whole insect, midgut, salivary gland and male reproductive organs. Nevertheless, the study of wild specimens would contribute a unique insight into the development of novel bioinsecticides. Given the recent VL outbreak in Argentina and the compelling need to develop appropriate control strategies, this study focused on wild male and female Lu. longipalpis from an Argentine endemic (Posadas, Misiones) and a Brazilian non-endemic (Lapinha Cave, Minas Gerais) VL location. In this study, total RNA was extracted from the sand flies, submitted to sequence independent amplification and high-throughput pyrosequencing. This is the first time an unbiased and comprehensive transcriptomic approach has been used to analyse an infectious disease vector in its natural environment. Transcripts identified in the sand flies showed characteristic profiles which correlated with the environment of origin and with taxa previously identified in these same specimens. Among these, various genes represented putative targets for vector control via RNA interference (RNAi).info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30677/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALFirst Comparative Transcriptomic Analysis of Wild Adult Male.pdfFirst Comparative Transcriptomic Analysis of Wild Adult Male.pdfapplication/pdf16203187https://www.arca.fiocruz.br/bitstream/icict/30677/2/First%20Comparative%20Transcriptomic%20Analysis%20of%20Wild%20Adult%20Male.pdf519304389bb1830504e1106650f20b95MD52TEXTFirst Comparative Transcriptomic Analysis of Wild Adult Male.pdf.txtFirst Comparative Transcriptomic Analysis of Wild Adult Male.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis
title First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis
spellingShingle First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis
McCarthy, Christina B.
Leishmaniasis
Visceral leishmaniasis
Leishmaniasis
Visceral leishmaniasis
title_short First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis
title_full First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis
title_fullStr First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis
title_full_unstemmed First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis
title_sort First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis
author McCarthy, Christina B.
author_facet McCarthy, Christina B.
Santini, María Soledad
Pimenta, Paulo Filemon Paolucci
Diambra, Luis A.
author_role author
author2 Santini, María Soledad
Pimenta, Paulo Filemon Paolucci
Diambra, Luis A.
author2_role author
author
author
dc.contributor.author.fl_str_mv McCarthy, Christina B.
Santini, María Soledad
Pimenta, Paulo Filemon Paolucci
Diambra, Luis A.
dc.subject.other.pt_BR.fl_str_mv Leishmaniasis
Visceral leishmaniasis
topic Leishmaniasis
Visceral leishmaniasis
Leishmaniasis
Visceral leishmaniasis
dc.subject.en.pt_BR.fl_str_mv Leishmaniasis
Visceral leishmaniasis
description Centro Regional de Estudios Genómicos. Facultad de Ciencias Exactas. Universidad Nacional de La Plata. Florencio Varela, Buenos Aires. Argentina/Departamento de Informática y Tecnología. Universidad Nacional del Noroeste de la Provincia de Buenos Aires. Pergamino, Buenos Aires, Argentina
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2018-12-18T17:16:20Z
dc.date.available.fl_str_mv 2018-12-18T17:16:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MCCARTHY, Christina B. et al. First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis. PLoS One.,v. 8, n. 3, e58645, 2013
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30677
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0058645
identifier_str_mv MCCARTHY, Christina B. et al. First comparative transcriptomic analysis of wild adult male and female Lutzomyia longipalpis, vector of visceral leishmaniasis. PLoS One.,v. 8, n. 3, e58645, 2013
1932-6203
10.1371/journal.pone.0058645
url https://www.arca.fiocruz.br/handle/icict/30677
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/30677/1/license.txt
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