Phenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristics
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/51760 |
Resumo: | Instituto René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Southwest National Primate Research Center. Texas Biomedical Research Institute. San Antonio, TX, United States/Faculdade de Minas. Belo Horizonte, MG, Brazil/ Faculdade de Ciências Médicas de Minas Gerais. Belo Horizonte, MG, Brazil. |
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Avelar, Renato SathlerAvelar, Danielle Marquete VitelliBarbosa, Armanda Moreira MattosoXavier, Marcelo Antônio PascoalSantos, Silvana Maria ElóiRocha, Ismael Artur da CostaCarvalho, Andréa TeixeiraDick Jr, Edward JVandeBerg, Jane FVandeBerg, John LMartins Filho, Olindo Assis2022-03-21T12:10:58Z2022-03-21T12:10:58Z2021AVELAR, Renato Sathler et al. Phenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristics. Front Cell Infect Microbiol., v. 11, 701930, 2021. doi: 10.3389/fcimb.2021.701930.2235-2988https://www.arca.fiocruz.br/handle/icict/5176010.3389/fcimb.2021.701930engFrontiers Media ASPhenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristicsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Southwest National Primate Research Center. Texas Biomedical Research Institute. San Antonio, TX, United States/Faculdade de Minas. Belo Horizonte, MG, Brazil/ Faculdade de Ciências Médicas de Minas Gerais. Belo Horizonte, MG, Brazil.Instituto René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Southwest National Primate Research Center. Texas Biomedical Research Institute. San Antonio, TX, United States.Faculdade de Minas. Belo Horizonte, MG, Brazil.Instituto René Rachou.Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Instituto René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Propedêutica Complementar. Belo Horizonte, MG, Brazil.Instituto René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Instituto René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.Southwest National Primate Research Center. Texas Biomedical Research Institute. San Antonio, TX, United States.Southwest National Primate Research Center. Texas Biomedical Research Institute. San Antonio, TX, United States.Southwest National Primate Research Center. Texas Biomedical Research Institute. San Antonio, TX, United States/South Texas Diabetes and Obesity Institute and Department of Human Genetics. School of Medicine. The University of Texas Rio Grande Valley. Brownsville/Harlingen/Edinburg, TX, United States/Center for Vector-Borne Diseases. The University of Texas Rio Grande Valley. Brownsville/Harlingen/Edinburg, TX, United States.Instituto René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil.We performed a detailed analysis of immunophenotypic features of circulating leukocytes and spleen cells from cynomolgus macaques that had been naturally infected with Trypanosoma cruzi, identifying their unique and shared characteristics in relation to cardiac histopathological lesion status. T. cruzi-infected macaques were categorized into three groups: asymptomatic [CCC(-)], with mild chronic chagasic cardiopathy [CCC(+)], or with moderate chronic chagasic cardiopathy [CCC(++)]. Our findings demonstrated significant differences in innate and adaptive immunity cells of the peripheral blood and spleen compartments, by comparison with non-infected controls. CCC(+) and CCC(++) hosts exhibited decreased frequencies of monocytes, NK and NKT-cell subsets in both compartments, and increased frequencies of activated CD8+ T-cells and GranA+/GranB+ cells. While a balanced cytokine profile (TNF/IL-10) was observed in peripheral blood of CCC(-) macaques, a predominant pro-inflammatory profile (increased levels of TNF and IFN/IL-10) was observed in both CCC(+) and CCC(++) subgroups. Our data demonstrated that cardiac histopathological features of T. cruzi-infected cynomolgus macaques are associated with perturbations of the immune system similarly to those observed in chagasic humans. These results provide further support for the validity of the cynomolgus macaque model for pre-clinical research on Chagas disease, and provide insights pertaining to the underlying immunological mechanisms involved in the progression of cardiac Chagas diseaseTrypanosoma cruzicardiac Chagas diseasecynomolgus macaquescytokinesimmune responsenon-human primatesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/51760/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALPhenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi_ Associations With Cardiac Histopathological Characteristics.pdfPhenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi_ Associations With Cardiac Histopathological Characteristics.pdfapplication/pdf4895427https://www.arca.fiocruz.br/bitstream/icict/51760/2/Phenotypic%20and%20Functional%20Signatures%20of%20Peripheral%20Blood%20and%20Spleen%20Compartments%20of%20Cynomolgus%20Macaques%20Infected%20With%20T.%20cruzi_%20Associations%20With%20Cardiac%20Histopathological%20Characteristics.pdfb047ec01aeb8fec490b4520202cc16bbMD52icict/517602022-03-21 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| dc.title.pt_BR.fl_str_mv |
Phenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristics |
| title |
Phenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristics |
| spellingShingle |
Phenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristics Avelar, Renato Sathler Trypanosoma cruzi cardiac Chagas disease cynomolgus macaques cytokines immune response non-human primates |
| title_short |
Phenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristics |
| title_full |
Phenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristics |
| title_fullStr |
Phenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristics |
| title_full_unstemmed |
Phenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristics |
| title_sort |
Phenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristics |
| author |
Avelar, Renato Sathler |
| author_facet |
Avelar, Renato Sathler Avelar, Danielle Marquete Vitelli Barbosa, Armanda Moreira Mattoso Xavier, Marcelo Antônio Pascoal Santos, Silvana Maria Elói Rocha, Ismael Artur da Costa Carvalho, Andréa Teixeira Dick Jr, Edward J VandeBerg, Jane F VandeBerg, John L Martins Filho, Olindo Assis |
| author_role |
author |
| author2 |
Avelar, Danielle Marquete Vitelli Barbosa, Armanda Moreira Mattoso Xavier, Marcelo Antônio Pascoal Santos, Silvana Maria Elói Rocha, Ismael Artur da Costa Carvalho, Andréa Teixeira Dick Jr, Edward J VandeBerg, Jane F VandeBerg, John L Martins Filho, Olindo Assis |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Avelar, Renato Sathler Avelar, Danielle Marquete Vitelli Barbosa, Armanda Moreira Mattoso Xavier, Marcelo Antônio Pascoal Santos, Silvana Maria Elói Rocha, Ismael Artur da Costa Carvalho, Andréa Teixeira Dick Jr, Edward J VandeBerg, Jane F VandeBerg, John L Martins Filho, Olindo Assis |
| dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi cardiac Chagas disease cynomolgus macaques cytokines immune response non-human primates |
| topic |
Trypanosoma cruzi cardiac Chagas disease cynomolgus macaques cytokines immune response non-human primates |
| description |
Instituto René Rachou. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/Southwest National Primate Research Center. Texas Biomedical Research Institute. San Antonio, TX, United States/Faculdade de Minas. Belo Horizonte, MG, Brazil/ Faculdade de Ciências Médicas de Minas Gerais. Belo Horizonte, MG, Brazil. |
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2021 |
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2021 |
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2022-03-21T12:10:58Z |
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2022-03-21T12:10:58Z |
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info:eu-repo/semantics/article |
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AVELAR, Renato Sathler et al. Phenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristics. Front Cell Infect Microbiol., v. 11, 701930, 2021. doi: 10.3389/fcimb.2021.701930. |
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https://www.arca.fiocruz.br/handle/icict/51760 |
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2235-2988 |
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10.3389/fcimb.2021.701930 |
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AVELAR, Renato Sathler et al. Phenotypic and Functional Signatures of Peripheral Blood and Spleen Compartments of Cynomolgus Macaques Infected With T. cruzi: Associations With Cardiac Histopathological Characteristics. Front Cell Infect Microbiol., v. 11, 701930, 2021. doi: 10.3389/fcimb.2021.701930. 2235-2988 10.3389/fcimb.2021.701930 |
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