Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/25599 |
Resumo: | Conselho Nacional de Desenvolvimento Científico e Tecnológico - Universal-MCTI/ CNPq, Grant number: process: 478322/2012-7; Instituto Gonçalo Moniz - FIOCRUZ– BA/BRAZIL, Grant number: 14/2012 |
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Santos, Maria Isabel Magalhães Andrade dosPacheco, Sidelcina RugieriStocker, AndreasSchinoni, Maria IsabelParaná, RaymundoReis, Mitermayer Galvão dosSilva, Luciano Kalabric2018-04-03T18:24:48Z2018-04-03T18:24:48Z2017SANTOS, M. I. M. A. et al. Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection. Journal of Medical Virology, v. 89, p. 1811–1816, 2017.0146-6615https://www.arca.fiocruz.br/handle/icict/2559910.1002/jmv.24853Conselho Nacional de Desenvolvimento Científico e Tecnológico - Universal-MCTI/ CNPq, Grant number: process: 478322/2012-7; Instituto Gonçalo Moniz - FIOCRUZ– BA/BRAZIL, Grant number: 14/2012Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Laboratório do Serviço de Gastro-Hepatologia de Pesquisa em Infectologia. Salvador, BA,BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Ambulatório Magalhães Neto. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospitalar Universitário Professor Edgard Santos. Ambulatório Magalhães Neto. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilThe Brazilian public health system (SUS) has provided antiviral drugs for chronic hepatitis B treatment for over 10 years, but a system for monitoring for drug-related resistance mutations is not available. Determine the presence of HBV mutations associated with resistance to nucleos(t)ide analogs among 81 patients with chronic HBV infection in Salvador-BA-Brazil. HBV-DNA was PCR amplified with primers deduced from the rt domain at the HBV P gene, the sequence extended 1032 bp (from amino acid 1 to 344-rt domain). Those sequences were submitted to the HBV drug resistance database to retrieve each mutation according to the genotype. HBV genotype A1 (85.2%) was the most prevalent, followed by genotype A2 (4.9%), F (6.2%), and C1, D2, and D4 (1.2% each). Six patients (7%) exhibited resistance mutations to LAM, ETV, and TDF: two with patterns L180M + M204V and four with other different patterns: L80I + L180M + M204I; L80V + L180M + M204V; M204I; A194T. All of these mutations were present in patients with genotype A (four A1 and two A2). In addition, four mutations in gene S (three cases with the sI195M mutation and one with the W196L mutation), were detected, corresponding to a rate of 6% of vaccine escape mutations. Althougth the small sample size, an association was found between the occurrence of HBV resistance mutations and HBeAg positivity, co-infection with HIV and a history of treatment for HBV and/or HIV.engWileyHBVVírus da hepatite BAnálogos nucleos (t) ideresistênciaHBVHepatitis B virusNucleos(t)ides analogsResistanceMutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25599/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSantos MI Mutations Associated With Drug Resistance....pdfSantos MI Mutations Associated With Drug Resistance....pdfapplication/pdf221088https://www.arca.fiocruz.br/bitstream/icict/25599/2/Santos%20MI%20Mutations%20Associated%20With%20Drug%20Resistance....pdfd625417727c6a24993cbef09721bb9a4MD52TEXTSantos MI Mutations Associated With Drug Resistance....pdf.txtSantos MI Mutations Associated With Drug Resistance....pdf.txtExtracted texttext/plain24557https://www.arca.fiocruz.br/bitstream/icict/25599/3/Santos%20MI%20Mutations%20Associated%20With%20Drug%20Resistance....pdf.txt2319996a2aaf94fd4f5562b601904482MD53icict/255992023-03-15 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| dc.title.pt_BR.fl_str_mv |
Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection |
| title |
Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection |
| spellingShingle |
Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection Santos, Maria Isabel Magalhães Andrade dos HBV Vírus da hepatite B Análogos nucleos (t) ide resistência HBV Hepatitis B virus Nucleos(t)ides analogs Resistance |
| title_short |
Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection |
| title_full |
Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection |
| title_fullStr |
Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection |
| title_full_unstemmed |
Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection |
| title_sort |
Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection |
| author |
Santos, Maria Isabel Magalhães Andrade dos |
| author_facet |
Santos, Maria Isabel Magalhães Andrade dos Pacheco, Sidelcina Rugieri Stocker, Andreas Schinoni, Maria Isabel Paraná, Raymundo Reis, Mitermayer Galvão dos Silva, Luciano Kalabric |
| author_role |
author |
| author2 |
Pacheco, Sidelcina Rugieri Stocker, Andreas Schinoni, Maria Isabel Paraná, Raymundo Reis, Mitermayer Galvão dos Silva, Luciano Kalabric |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Maria Isabel Magalhães Andrade dos Pacheco, Sidelcina Rugieri Stocker, Andreas Schinoni, Maria Isabel Paraná, Raymundo Reis, Mitermayer Galvão dos Silva, Luciano Kalabric |
| dc.subject.other.pt_BR.fl_str_mv |
HBV Vírus da hepatite B Análogos nucleos (t) ide resistência |
| topic |
HBV Vírus da hepatite B Análogos nucleos (t) ide resistência HBV Hepatitis B virus Nucleos(t)ides analogs Resistance |
| dc.subject.en.pt_BR.fl_str_mv |
HBV Hepatitis B virus Nucleos(t)ides analogs Resistance |
| description |
Conselho Nacional de Desenvolvimento Científico e Tecnológico - Universal-MCTI/ CNPq, Grant number: process: 478322/2012-7; Instituto Gonçalo Moniz - FIOCRUZ– BA/BRAZIL, Grant number: 14/2012 |
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2017 |
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2017 |
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2018-04-03T18:24:48Z |
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2018-04-03T18:24:48Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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SANTOS, M. I. M. A. et al. Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection. Journal of Medical Virology, v. 89, p. 1811–1816, 2017. |
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https://www.arca.fiocruz.br/handle/icict/25599 |
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0146-6615 |
| dc.identifier.doi.none.fl_str_mv |
10.1002/jmv.24853 |
| identifier_str_mv |
SANTOS, M. I. M. A. et al. Mutations associated with drug resistance and prevalence of vaccine escape mutations in patients with chronic hepatitis B infection. Journal of Medical Virology, v. 89, p. 1811–1816, 2017. 0146-6615 10.1002/jmv.24853 |
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eng |
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openAccess |
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Wiley |
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Wiley |
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