Cytotoxicity in patients with different clinical forms of Chagas' disease

Detalhes bibliográficos
Autor(a) principal: Brodskyn, Claudia Ida
Data de Publicação: 1996
Outros Autores: Barral, Aldina Maria Prado, Bulhões, M. A., Souto, T., Machado, W. C., Barral Netto, Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7454
Resumo: Universidade Federal da Bahia. Hospital Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
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spelling Brodskyn, Claudia IdaBarral, Aldina Maria PradoBulhões, M. A.Souto, T.Machado, W. C.Barral Netto, Manoel2014-03-26T17:02:05Z2014-03-26T17:02:05Z1996BRODSKYN, C. I. et al. ytotoxicity in patients with different clinical forms of Chagas' disease. Clinical and Experimental Immunology, v. 105, n. 3, p. 450-455, 1996.0009-9104https://www.arca.fiocruz.br/handle/icict/7454engBlackwell ScienceCytotoxicity in patients with different clinical forms of Chagas' diseaseinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal da Bahia. Hospital Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Integrado de Microbiologia e Imunoregulação. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Integrado de Microbiologia e Imunoregulação. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Integrado de Microbiologia e Imunoregulação. Salvador, BA, BrasilUniversidade Federal da Bahia. Hospital Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Integrado de Microbiologia e Imunoregulação. Salvador, BA, BrasilThere are few studies on cell-mediated cytotoxicity in human Chagas' disease. In the present study, we evaluated peripheral blood mononuclear cell (PBMC) cytotoxicity activity from chagasic patients with different clinical forms of disease. To verify the cytotoxic response, we performed cell lysis assays using 51Cr-labelled K562 cells as targets. Results are reported as lytic units (LU = number of cells required for 30% lysis) per million PBMC. Exposure of patients' PBMC to Trypanosoma cruzi antigen led to an increase in cytotoxic activity compared with unstimulated patient cells against K562. Asymptomatic cardiomyopathy patients had higher responses (37.8 +/- 5.0 LU/10(6) PBMC; mean +/- s.d.) than indeterminate (11.5 +/- 3.6 LU/10(6) and symptomatic cardiomyopathy (7.8 +/- 2.5 LU/10(6)). Normal control PBMC stimulated with T. cruzi antigen had 4.36 +/- 1.31 LU/10(6)) PBMC against K562. Addition of recombinant interferon-gamma (IFN-gamma) did not lead to significant increase in cytotoxicity in any group of patients. On the other hand, recombinant human IL-12 significantly increased cytotoxic responses from symptomatic cardiomyopathy patients and normal controls who presented low levels of cytotoxicity induced by T. cruzi antigen. The combined use of IL-12 and a neutralizing anti-IFN-gamma antibody did not change IL-12-induced cytotoxic responses, showing the direct role of this cytokine on natural killer (NK) cells. NK cells were the main cells responsible for the lysis of K562 target cells as evidenced by testing cell subsets following magnetic cell sorting. These data demonstrate that chagasic patients with different clinical forms of disease have PBMC which respond to T. cruzi antigen with a cytotoxic response, and this response is up-regulated by IL-12.Chagas' diseaseCitotoxicityInterferon-gammaIl-12Doença de Chagas/imunologiaCitotoxicidade ImunológicaAdultoIdosoAnimaisTestes Imunológicos de CitotoxicidadeHumanosmunofenotipagemInterferon gama/fisiologiaInterleucina-12/fisiologiaLeucemia Eritroblástica Aguda/imunologiaMeia-IdadeCélulas Tumorais Cultivadasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALBrodskyn C Cytotoxicity in....pdfBrodskyn C Cytotoxicity in....pdfapplication/pdf213792https://www.arca.fiocruz.br/bitstream/icict/7454/1/Brodskyn%20C%20Cytotoxicity%20in....pdf27f7cef3a5edee03cb9023a4206d0672MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7454/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTBrodskyn C Cytotoxicity in....pdf.txtBrodskyn C Cytotoxicity in....pdf.txtExtracted texttext/plain27029https://www.arca.fiocruz.br/bitstream/icict/7454/5/Brodskyn%20C%20Cytotoxicity%20in....pdf.txt33f01ee2d9bb44fdc45607ff9f56a532MD55THUMBNAILBrodskyn C Cytotoxicity in....pdf.jpgBrodskyn C Cytotoxicity in....pdf.jpgGenerated Thumbnailimage/jpeg1786https://www.arca.fiocruz.br/bitstream/icict/7454/4/Brodskyn%20C%20Cytotoxicity%20in....pdf.jpg4a8310600689e8c3cee7b67477422a5fMD54icict/74542023-03-15 14:33:22.648oai:www.arca.fiocruz.br:icict/7454TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:22Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Cytotoxicity in patients with different clinical forms of Chagas' disease
title Cytotoxicity in patients with different clinical forms of Chagas' disease
spellingShingle Cytotoxicity in patients with different clinical forms of Chagas' disease
Brodskyn, Claudia Ida
Chagas' disease
Citotoxicity
Interferon-gamma
Il-12
Doença de Chagas/imunologia
Citotoxicidade Imunológica
Adulto
Idoso
Animais
Testes Imunológicos de Citotoxicidade
Humanos
munofenotipagem
Interferon gama/fisiologia
Interleucina-12/fisiologia
Leucemia Eritroblástica Aguda/imunologia
Meia-Idade
Células Tumorais Cultivadas
title_short Cytotoxicity in patients with different clinical forms of Chagas' disease
title_full Cytotoxicity in patients with different clinical forms of Chagas' disease
title_fullStr Cytotoxicity in patients with different clinical forms of Chagas' disease
title_full_unstemmed Cytotoxicity in patients with different clinical forms of Chagas' disease
title_sort Cytotoxicity in patients with different clinical forms of Chagas' disease
author Brodskyn, Claudia Ida
author_facet Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Bulhões, M. A.
Souto, T.
Machado, W. C.
Barral Netto, Manoel
author_role author
author2 Barral, Aldina Maria Prado
Bulhões, M. A.
Souto, T.
Machado, W. C.
Barral Netto, Manoel
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Brodskyn, Claudia Ida
Barral, Aldina Maria Prado
Bulhões, M. A.
Souto, T.
Machado, W. C.
Barral Netto, Manoel
dc.subject.en.pt_BR.fl_str_mv Chagas' disease
Citotoxicity
Interferon-gamma
Il-12
topic Chagas' disease
Citotoxicity
Interferon-gamma
Il-12
Doença de Chagas/imunologia
Citotoxicidade Imunológica
Adulto
Idoso
Animais
Testes Imunológicos de Citotoxicidade
Humanos
munofenotipagem
Interferon gama/fisiologia
Interleucina-12/fisiologia
Leucemia Eritroblástica Aguda/imunologia
Meia-Idade
Células Tumorais Cultivadas
dc.subject.decs.pt_BR.fl_str_mv Doença de Chagas/imunologia
Citotoxicidade Imunológica
Adulto
Idoso
Animais
Testes Imunológicos de Citotoxicidade
Humanos
munofenotipagem
Interferon gama/fisiologia
Interleucina-12/fisiologia
Leucemia Eritroblástica Aguda/imunologia
Meia-Idade
Células Tumorais Cultivadas
description Universidade Federal da Bahia. Hospital Prof. Edgard Santos. Serviço de Imunologia. Salvador, BA, Brasil
publishDate 1996
dc.date.issued.fl_str_mv 1996
dc.date.accessioned.fl_str_mv 2014-03-26T17:02:05Z
dc.date.available.fl_str_mv 2014-03-26T17:02:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BRODSKYN, C. I. et al. ytotoxicity in patients with different clinical forms of Chagas' disease. Clinical and Experimental Immunology, v. 105, n. 3, p. 450-455, 1996.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7454
dc.identifier.issn.none.fl_str_mv 0009-9104
identifier_str_mv BRODSKYN, C. I. et al. ytotoxicity in patients with different clinical forms of Chagas' disease. Clinical and Experimental Immunology, v. 105, n. 3, p. 450-455, 1996.
0009-9104
url https://www.arca.fiocruz.br/handle/icict/7454
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Blackwell Science
publisher.none.fl_str_mv Blackwell Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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