Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil

Detalhes bibliográficos
Autor(a) principal: Arruda, Sérgio Marcos
Data de Publicação: 1998
Outros Autores: Chalhoub, Marcelo, Cardoso, Silvia Andrade, Barral Netto, Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7445
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imuno-regulação e Microbiologia. Salvador, BA, Brasil
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spelling Arruda, Sérgio MarcosChalhoub, MarceloCardoso, Silvia AndradeBarral Netto, Manoel2014-03-25T17:46:14Z2014-03-25T17:46:14Z1998ARRUDA, S. et al. Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil. Acta Tropica, v. 71, p. 1-15, 1998.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/7445engElsevier Science B.VCell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imuno-regulação e Microbiologia. Salvador, BA, BrasilSESAB. Hospital Octávio Mangabeira - HOM. Salvador, BA, BrasilSESAB. Hospital Octávio Mangabeira - HOM. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imuno-regulação e Microbiologia. Salvador, BA, BrasilEvaluating human immune response to defined Mycobacterium tuberculosis antigens in patients with different clinical forms of tuberculosis may help in elucidating pathogenesis and in vaccine development. In the present report we evaluated the lymphocyte proliferation, cytokine production and natural killer cell cytotoxicity as parameters to screen four mycobacterial recombinant antigens. Pleural fluid mononuclear cells (PFMC) and peripheral blood mononuclear cells (PBMC) from 13 HIV-negative patients with tuberculous pleurisy, living in a tropical region of Brazil were used in these assays. Crude M. tuberculosis antigen and recombinant 70-, 65- and 38-kDa mycobacterial antigens, induced greater proliferation in PFMC than in PBMC. IFN-gamma, TNF-alpha, IL-4 and IL-10 were evaluated in the PFMC supernatants stimulated by these antigens. Both crude and 70-kDa antigens induced higher levels of IFN-gamma, TNF-alpha and IL-10. There was a significant positive correlation between IFN-gamma and the proliferative response induced by crude M. tuberculosis antigen, and an inverse correlation was identified between IL-10 and cell proliferation. IL-4 was not detected in the supernatants of pleural fluid mononuclear cell cultures stimulated by either crude, or recombinant antigens. TNF-alpha was detected in variable amounts in supernatants of PFMC stimulated by all antigens tested. Natural killer cytotoxicity was induced by both crude and 70-kDa antigen. Our results demonstrate that cells present at the site of disease recognized three of the antigens screened, as shown by lymphocyte proliferation and production of regulatory and inflammatory cytokines, and the results obtained with PFMC were consistently higher than those obtained with homologous PBMC.Antígenos de Bactérias/imunologiaCitocinas/sangueCitotoxicidade Imunológica/imunologiaAtivação Linfocitária/imunologiaMycobacterium tuberculosis/imunologiaTuberculose Pleural/imunologiaAnticorpos MonoclonaisCitotoxicidade Imunológica/fisiologiaEnsaio de Imunoadsorção EnzimáticaHumanosImunidade Celular/imunologiaInterferon gama/sangueInterleucina-10/sangueCélulas Matadoras Naturais/imunologiaDerrame Pleural/imunologiaProteínas Recombinantes/imunologiaTuberculose Pleural/microbiologiaFator de Necrose Tumoral alfa/imunologiainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALArruda S Cell-Mediated....pdfArruda S Cell-Mediated....pdfapplication/pdf157287https://www.arca.fiocruz.br/bitstream/icict/7445/1/Arruda%20S%20Cell-Mediated....pdfea67f53bc935e00dbf95e05ba191d583MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7445/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTArruda S Cell-Mediated....pdf.txtArruda S Cell-Mediated....pdf.txtExtracted texttext/plain34433https://www.arca.fiocruz.br/bitstream/icict/7445/5/Arruda%20S%20Cell-Mediated....pdf.txt1a265b46c16cf3aa7137f96ca57580c4MD55THUMBNAILArruda S Cell-Mediated....pdf.jpgArruda S Cell-Mediated....pdf.jpgGenerated Thumbnailimage/jpeg1533https://www.arca.fiocruz.br/bitstream/icict/7445/4/Arruda%20S%20Cell-Mediated....pdf.jpg7db3e66d30dcac1aa64cce9b0e812599MD54icict/74452023-03-15 14:33:00.654oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil
title Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil
spellingShingle Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil
Arruda, Sérgio Marcos
Antígenos de Bactérias/imunologia
Citocinas/sangue
Citotoxicidade Imunológica/imunologia
Ativação Linfocitária/imunologia
Mycobacterium tuberculosis/imunologia
Tuberculose Pleural/imunologia
Anticorpos Monoclonais
Citotoxicidade Imunológica/fisiologia
Ensaio de Imunoadsorção Enzimática
Humanos
Imunidade Celular/imunologia
Interferon gama/sangue
Interleucina-10/sangue
Células Matadoras Naturais/imunologia
Derrame Pleural/imunologia
Proteínas Recombinantes/imunologia
Tuberculose Pleural/microbiologia
Fator de Necrose Tumoral alfa/imunologia
title_short Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil
title_full Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil
title_fullStr Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil
title_full_unstemmed Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil
title_sort Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil
author Arruda, Sérgio Marcos
author_facet Arruda, Sérgio Marcos
Chalhoub, Marcelo
Cardoso, Silvia Andrade
Barral Netto, Manoel
author_role author
author2 Chalhoub, Marcelo
Cardoso, Silvia Andrade
Barral Netto, Manoel
author2_role author
author
author
dc.contributor.author.fl_str_mv Arruda, Sérgio Marcos
Chalhoub, Marcelo
Cardoso, Silvia Andrade
Barral Netto, Manoel
dc.subject.decs.pt_BR.fl_str_mv Antígenos de Bactérias/imunologia
Citocinas/sangue
Citotoxicidade Imunológica/imunologia
Ativação Linfocitária/imunologia
Mycobacterium tuberculosis/imunologia
Tuberculose Pleural/imunologia
Anticorpos Monoclonais
Citotoxicidade Imunológica/fisiologia
Ensaio de Imunoadsorção Enzimática
Humanos
Imunidade Celular/imunologia
Interferon gama/sangue
Interleucina-10/sangue
Células Matadoras Naturais/imunologia
Derrame Pleural/imunologia
Proteínas Recombinantes/imunologia
Tuberculose Pleural/microbiologia
Fator de Necrose Tumoral alfa/imunologia
topic Antígenos de Bactérias/imunologia
Citocinas/sangue
Citotoxicidade Imunológica/imunologia
Ativação Linfocitária/imunologia
Mycobacterium tuberculosis/imunologia
Tuberculose Pleural/imunologia
Anticorpos Monoclonais
Citotoxicidade Imunológica/fisiologia
Ensaio de Imunoadsorção Enzimática
Humanos
Imunidade Celular/imunologia
Interferon gama/sangue
Interleucina-10/sangue
Células Matadoras Naturais/imunologia
Derrame Pleural/imunologia
Proteínas Recombinantes/imunologia
Tuberculose Pleural/microbiologia
Fator de Necrose Tumoral alfa/imunologia
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Imuno-regulação e Microbiologia. Salvador, BA, Brasil
publishDate 1998
dc.date.issued.fl_str_mv 1998
dc.date.accessioned.fl_str_mv 2014-03-25T17:46:14Z
dc.date.available.fl_str_mv 2014-03-25T17:46:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARRUDA, S. et al. Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil. Acta Tropica, v. 71, p. 1-15, 1998.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7445
dc.identifier.issn.none.fl_str_mv 0001-706X
identifier_str_mv ARRUDA, S. et al. Cell-mediated immune responses and cytotoxicity to mycobacterial antigens in patients with tuberculous pleurisy in Brazil. Acta Tropica, v. 71, p. 1-15, 1998.
0001-706X
url https://www.arca.fiocruz.br/handle/icict/7445
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier Science B.V
publisher.none.fl_str_mv Elsevier Science B.V
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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