Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients

Detalhes bibliográficos
Autor(a) principal: Mello, Francisco C. A.
Data de Publicação: 2012
Outros Autores: Lago, Bárbara V., Lewis-Ximenez, Lia L., Fernandes, Carlos A., Gomes, Selma A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16871
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Mello, Francisco C. A.Lago, Bárbara V.Lewis-Ximenez, Lia L.Fernandes, Carlos A.Gomes, Selma A.2017-01-17T16:03:07Z2017-01-17T16:03:07Z2012MELLO, Francisco C. A. et al. Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients. BMC Microbiology, v.12, n.96, 9p, 2012.1471-2180https://www.arca.fiocruz.br/handle/icict/1687110.1186/1471-2180-12-96engBioMed CentralLamivudinaTratamentoInfecção crônica pelo vírus da hepatite B (HBV)Terapias antiviraishepatitis Btreatmentchronic hepatitis B virus (HBV) infectionYMDD variantsantiviral therapiesLamiduvineDetection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patientsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional para Hepatites Virais. Rio de Janeiro, RJ. Brasil.Laboratório Central de Saúde Pública Noel Nutels, Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Lamivudine (LAM) is associated with the highest known rate of resistance mutations among nucleotide analogs used to treat chronic hepatitis B virus (HBV) infection. Despite this, LAM continues in widespread use, especially in combination therapies. The primary LAM resistance mutation (rtM204V/I) occurs in the YMDD motif of HBV polymerase. The aim of this study was to characterize Brazilian HBV isolates from acute and chronic cases by direct sequencing, and to identify HBV quasispecies in the YMDD motif using a pyrosequencing method capable of detecting single-nucleotide polymorphisms. HBV DNA from serum samples of 20 individuals with acute HBV infection and 44 with chronic infection undergoing antiviral therapies containing LAM were analyzed by direct sequencing and pyrosequencing methods.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16871/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALfrancisco_mello_etal_IOC_2012.pdsf.pdffrancisco_mello_etal_IOC_2012.pdsf.pdfapplication/pdf849121https://www.arca.fiocruz.br/bitstream/icict/16871/2/francisco_mello_etal_IOC_2012.pdsf.pdf7d7182267dededbc317b82f303e9a1eaMD52TEXTfrancisco_mello_etal_IOC_2012.pdsf.pdf.txtfrancisco_mello_etal_IOC_2012.pdsf.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients
title Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients
spellingShingle Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients
Mello, Francisco C. A.
Lamivudina
Tratamento
Infecção crônica pelo vírus da hepatite B (HBV)
Terapias antivirais
hepatitis B
treatment
chronic hepatitis B virus (HBV) infection
YMDD variants
antiviral therapies
Lamiduvine
title_short Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients
title_full Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients
title_fullStr Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients
title_full_unstemmed Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients
title_sort Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients
author Mello, Francisco C. A.
author_facet Mello, Francisco C. A.
Lago, Bárbara V.
Lewis-Ximenez, Lia L.
Fernandes, Carlos A.
Gomes, Selma A.
author_role author
author2 Lago, Bárbara V.
Lewis-Ximenez, Lia L.
Fernandes, Carlos A.
Gomes, Selma A.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mello, Francisco C. A.
Lago, Bárbara V.
Lewis-Ximenez, Lia L.
Fernandes, Carlos A.
Gomes, Selma A.
dc.subject.other.pt_BR.fl_str_mv Lamivudina
Tratamento
Infecção crônica pelo vírus da hepatite B (HBV)
Terapias antivirais
topic Lamivudina
Tratamento
Infecção crônica pelo vírus da hepatite B (HBV)
Terapias antivirais
hepatitis B
treatment
chronic hepatitis B virus (HBV) infection
YMDD variants
antiviral therapies
Lamiduvine
dc.subject.en.pt_BR.fl_str_mv hepatitis B
treatment
chronic hepatitis B virus (HBV) infection
YMDD variants
antiviral therapies
Lamiduvine
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-01-17T16:03:07Z
dc.date.available.fl_str_mv 2017-01-17T16:03:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MELLO, Francisco C. A. et al. Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients. BMC Microbiology, v.12, n.96, 9p, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16871
dc.identifier.issn.pt_BR.fl_str_mv 1471-2180
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2180-12-96
identifier_str_mv MELLO, Francisco C. A. et al. Detection of mixed populations of wild-type and YMDD hepatitis B variants by pyrosequencing in acutely and chronically infected patients. BMC Microbiology, v.12, n.96, 9p, 2012.
1471-2180
10.1186/1471-2180-12-96
url https://www.arca.fiocruz.br/handle/icict/16871
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/16871/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/16871/2/francisco_mello_etal_IOC_2012.pdsf.pdf
https://www.arca.fiocruz.br/bitstream/icict/16871/3/francisco_mello_etal_IOC_2012.pdsf.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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