Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus

Detalhes bibliográficos
Autor(a) principal: Feder, D.
Data de Publicação: 1998
Outros Autores: Salles, J. M., Garcia, Eloi S., Azambuja, Patricia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43955
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Feder, D.Salles, J. M.Garcia, Eloi S.Azambuja, Patricia2020-10-13T13:09:17Z2020-10-13T13:09:17Z1998FEDER, D. et al. Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n. 6, p. 823-826, Nov./Dec. 1998.0074-0276https://www.arca.fiocruz.br/handle/icict/4395510.1590/S0074-027619980006000231678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.RhodniusCorpo gordoHaemolymphProteaseProphenoloxidaseTriatominaFat bodyHaemolymphRhodniusProteaseProphenoloxidaseTriatomineHaemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Analysis of zymograms with SDS-polyacrilamide gel electrophoresis containing gelatin as substrate, and performed on samples of haemolymph or fat body taken from Rhodnius prolixus inoculated or not with Enterobacter cloacae, demonstrated distinct patterns of protease activities: (i) in the haemolymph two proteases were induced in insects inoculated with bacteria; (ii) two proteases were detected in the fat bodies derived from non-inoculated controls or insect inoculated with sterile culture medium; (iii) haemolymph and fat body had both the same apparent molecular weights proteases (46 and 56 kDa); and (iv) these enzymes were characterized as metallo-proteases. The association of these enzymes in Rhodnius infected with bacteria was discussed.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43955/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALESGarcia_PAzambuja_etal_IOC_1998.pdfESGarcia_PAzambuja_etal_IOC_1998.pdfapplication/pdf104509https://www.arca.fiocruz.br/bitstream/icict/43955/2/ESGarcia_PAzambuja_etal_IOC_1998.pdf0e2104f4eb03eea6bf73188122124897MD52TEXTESGarcia_PAzambuja_etal_IOC_1998.pdf.txtESGarcia_PAzambuja_etal_IOC_1998.pdf.txtExtracted texttext/plain14999https://www.arca.fiocruz.br/bitstream/icict/43955/3/ESGarcia_PAzambuja_etal_IOC_1998.pdf.txt46d82eb8f96d0605b017f4dde55edf17MD53icict/439552023-09-05 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dc.title.pt_BR.fl_str_mv Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus
title Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus
spellingShingle Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus
Feder, D.
Rhodnius
Corpo gordo
Haemolymph
Protease
Prophenoloxidase
Triatomina
Fat body
Haemolymph
Rhodnius
Protease
Prophenoloxidase
Triatomine
title_short Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus
title_full Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus
title_fullStr Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus
title_full_unstemmed Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus
title_sort Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus
author Feder, D.
author_facet Feder, D.
Salles, J. M.
Garcia, Eloi S.
Azambuja, Patricia
author_role author
author2 Salles, J. M.
Garcia, Eloi S.
Azambuja, Patricia
author2_role author
author
author
dc.contributor.author.fl_str_mv Feder, D.
Salles, J. M.
Garcia, Eloi S.
Azambuja, Patricia
dc.subject.other.pt_BR.fl_str_mv Rhodnius
Corpo gordo
Haemolymph
Protease
Prophenoloxidase
Triatomina
topic Rhodnius
Corpo gordo
Haemolymph
Protease
Prophenoloxidase
Triatomina
Fat body
Haemolymph
Rhodnius
Protease
Prophenoloxidase
Triatomine
dc.subject.en.pt_BR.fl_str_mv Fat body
Haemolymph
Rhodnius
Protease
Prophenoloxidase
Triatomine
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 1998
dc.date.issued.fl_str_mv 1998
dc.date.accessioned.fl_str_mv 2020-10-13T13:09:17Z
dc.date.available.fl_str_mv 2020-10-13T13:09:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FEDER, D. et al. Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n. 6, p. 823-826, Nov./Dec. 1998.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43955
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761998000600023
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv FEDER, D. et al. Haemolymph and Fat Body Metallo-protease Associated with Enterobacter cloacae Infection in the Bloodsucking Insect, Rhodnius prolixus. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 93, n. 6, p. 823-826, Nov./Dec. 1998.
0074-0276
10.1590/S0074-02761998000600023
1678-8060
url https://www.arca.fiocruz.br/handle/icict/43955
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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