HAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous System

Detalhes bibliográficos
Autor(a) principal: Gudo, Eduardo Samo
Data de Publicação: 2015
Outros Autores: Barbosa, Suse Dayse Silva, Lacerda, Leandra Linhares, Alves, Marcelo Ribeiro, Corte-Real, Suzana, Bou-Habib, Dumith Chequer, Savino, Wilson
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13429
Resumo: Marcelo Ribeiro Alves. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento.
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spelling Gudo, Eduardo SamoBarbosa, Suse Dayse SilvaLacerda, Leandra LinharesAlves, Marcelo RibeiroCorte-Real, SuzanaBou-Habib, Dumith ChequerSavino, Wilson2016-03-31T18:02:26Z2016-03-31T18:02:26Z2015GUDO, Eduardo Samo et al. HAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous System. Virology Journal, v. 12, n. 165, p. 1-12, 2015.1743-422Xhttps://www.arca.fiocruz.br/handle/icict/1342910.1186/s12985-015-0398-xMarcelo Ribeiro Alves. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento.Ministério da Saúde. Instituto Nacional de Saúde. Maputo, Mozambique / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Background: The mechanisms through which HTLV-1 leads to and maintains damage in the central nervous system of patients undergoing HTLV-1 associated myelopathy/tropical spastic paraparesis (HAM/TSP) are still poorly understood. In recent years, increasing evidence indicates that, not only lymphocytes but also glial cells, in particular astrocytes, play a role in the pathophysiology of HAM/TSP. In this study we used a model of co-culture between human HTLV-1-infected (CIB and C91PL) and non-infected (CEM) T lymphocyte cell lines and astrocyte (U251 and U87) cell lines to mimic the in vivo T cell-astrocyte interactions. Results: We first observed that CIB and C91PL adhere strongly to cultured astrocytes cell lines, and that co-cultures of HTLV-1 infected and astrocyte cell lines cells resulted in rapid syncytium formation, accompanied by severe morphological alterations and increased apoptotic cell death of astrocyte cells. Additionally, cultures of astrocyte cell lines in presence of supernatants harvested from HTLV-1-infected T cell cultures resulted in significant increase in the mRNA of CCL2, CXCL1, CXCL2, CXCL3, CXCL10, IL-13, IL-8, NFKB1, TLR4, TNF, MMP8 and VCAM1, as compared with the values obtained when we applied supernatants of non-infected T- cell lines. Lastly, soluble factors secreted by cultured astrocytic cell lines primed through 1-h interaction with infected T cell lines, further enhanced migratory responses, as compared to the effect seen when supernatants from astrocytic cell lines were primed with non-infected T cell lines. Conclusion: Collectively, our results show that HTLV-1 infected T lymphocyte cell lines interact strongly with astrocyte cell lines, leading to astrocyte damage and increased secretion of attracting cytokines, which in turn may participate in the further attraction of HTLV-1-infected T cells into central nervous system (CNS), thus amplifying and prolonging the immune damage of CNS.engBioMed CentralHAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous Systeminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAstrocytesHTLV-1-infected lymphocytesExtracellular matrixChemokinesIntegrinsIntegrinasQuimiocinasMatriz ExtracelularAstrócitosVírus 1 Linfotrópico T Humanoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv HAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous System
title HAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous System
spellingShingle HAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous System
Gudo, Eduardo Samo
Astrocytes
HTLV-1-infected lymphocytes
Extracellular matrix
Chemokines
Integrins
Integrinas
Quimiocinas
Matriz Extracelular
Astrócitos
Vírus 1 Linfotrópico T Humano
title_short HAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous System
title_full HAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous System
title_fullStr HAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous System
title_full_unstemmed HAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous System
title_sort HAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous System
author Gudo, Eduardo Samo
author_facet Gudo, Eduardo Samo
Barbosa, Suse Dayse Silva
Lacerda, Leandra Linhares
Alves, Marcelo Ribeiro
Corte-Real, Suzana
Bou-Habib, Dumith Chequer
Savino, Wilson
author_role author
author2 Barbosa, Suse Dayse Silva
Lacerda, Leandra Linhares
Alves, Marcelo Ribeiro
Corte-Real, Suzana
Bou-Habib, Dumith Chequer
Savino, Wilson
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gudo, Eduardo Samo
Barbosa, Suse Dayse Silva
Lacerda, Leandra Linhares
Alves, Marcelo Ribeiro
Corte-Real, Suzana
Bou-Habib, Dumith Chequer
Savino, Wilson
dc.subject.en.pt_BR.fl_str_mv Astrocytes
HTLV-1-infected lymphocytes
Extracellular matrix
Chemokines
Integrins
topic Astrocytes
HTLV-1-infected lymphocytes
Extracellular matrix
Chemokines
Integrins
Integrinas
Quimiocinas
Matriz Extracelular
Astrócitos
Vírus 1 Linfotrópico T Humano
dc.subject.decs.pt_BR.fl_str_mv Integrinas
Quimiocinas
Matriz Extracelular
Astrócitos
Vírus 1 Linfotrópico T Humano
description Marcelo Ribeiro Alves. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-31T18:02:26Z
dc.date.available.fl_str_mv 2016-03-31T18:02:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GUDO, Eduardo Samo et al. HAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous System. Virology Journal, v. 12, n. 165, p. 1-12, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13429
dc.identifier.issn.none.fl_str_mv 1743-422X
dc.identifier.doi.none.fl_str_mv 10.1186/s12985-015-0398-x
identifier_str_mv GUDO, Eduardo Samo et al. HAM/TSP-derived HTLV-1-infected T cell lines promote morphological and functional changes in human astrocytes cell lines: possible role in the enhanced T cells recruitment into Central Nervous System. Virology Journal, v. 12, n. 165, p. 1-12, 2015.
1743-422X
10.1186/s12985-015-0398-x
url https://www.arca.fiocruz.br/handle/icict/13429
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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