The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle

Detalhes bibliográficos
Autor(a) principal: Ricciardi, Simona
Data de Publicação: 2022
Outros Autores: Guarino, Andrea Maria, Giaquinto, Laura, Polishchuk, Elena V., Santoro, Michele, Di Tulio, Giuseppe, Wilson, Cathal, Panariello, Francesco, Soares, Vinicius C., Dias, Suelen S. G., Santos, Julia C., Souza, Thiago Moreno L., Fusco, Giovanna, Viscardi, Maurizio, Brandi, Sergio, Bozza, Patrícia Torres, Polishchuk, Roman S., Venditti, Rossella, Matteis, Maria Antonieta De
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/53156
Resumo: This is a PDF file of a peer-reviewed paper that has been accepted for publication. Although unedited, the content has been subjected to preliminary formatting. Nature is providing this early version of the typeset paper as a service to our authors and readers. The text and figures will undergo copyediting and a proof review before the paper is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers apply.
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spelling Ricciardi, SimonaGuarino, Andrea MariaGiaquinto, LauraPolishchuk, Elena V.Santoro, MicheleDi Tulio, GiuseppeWilson, CathalPanariello, FrancescoSoares, Vinicius C.Dias, Suelen S. G.Santos, Julia C.Souza, Thiago Moreno L.Fusco, GiovannaViscardi, MaurizioBrandi, SergioBozza, Patrícia TorresPolishchuk, Roman S.Venditti, RossellaMatteis, Maria Antonieta De2022-06-09T18:48:25Z2022-06-09T18:48:25Z2022RICCIARDI, Simona et al. The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle. Nature, p. 1-42, May 2022.1476-4687https://www.arca.fiocruz.br/handle/icict/5315610.1038/s41586-022-04835-6This is a PDF file of a peer-reviewed paper that has been accepted for publication. Although unedited, the content has been subjected to preliminary formatting. Nature is providing this early version of the typeset paper as a service to our authors and readers. The text and figures will undergo copyediting and a proof review before the paper is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers apply.Telethon Institute of Genetics and Medicine, TIGEM, Pozzuoli (Naples) Italy / Dept. Molecular Medicine and Medical Biotechnology, University of Naples Federico II, Italy.Telethon Institute of Genetics and Medicine, TIGEM, Pozzuoli (Naples) Italy.Telethon Institute of Genetics and Medicine, TIGEM, Pozzuoli (Naples) Italy.Telethon Institute of Genetics and Medicine, TIGEM, Pozzuoli (Naples) Italy.Telethon Institute of Genetics and Medicine, TIGEM, Pozzuoli (Naples) Italy.Telethon Institute of Genetics and Medicine, TIGEM, Pozzuoli (Naples) Italy.Telethon Institute of Genetics and Medicine, TIGEM, Pozzuoli (Naples) Italy.Telethon Institute of Genetics and Medicine, TIGEM, Pozzuoli (Naples) Italy.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Programa de Imunologia e Inflamação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnologico em Saúde. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Inovação em Doenças de Populações Negligenciadas. Rio de Janeiro, RJ, Brasil.Istituto Zooprofilattico Sperimentale del Mezzogiorno. 80055 Portici (Naples). Italy.Istituto Zooprofilattico Sperimentale del Mezzogiorno. 80055 Portici (Naples). Italy.Istituto Zooprofilattico Sperimentale del Mezzogiorno. 80055 Portici (Naples). Italy.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Telethon Institute of Genetics and Medicine, TIGEM, Pozzuoli (Naples) Italy.Telethon Institute of Genetics and Medicine, TIGEM, Pozzuoli (Naples) Italy / Dept. Molecular Medicine and Medical Biotechnology, University of Naples Federico II. Italy.Telethon Institute of Genetics and Medicine, TIGEM, Pozzuoli (Naples) Italy / Dept. Molecular Medicine and Medical Biotechnology, University of Naples Federico II. Italy.SARS-CoV-2, like other coronaviruses, builds a membrane-bound replication organelle (RO) to enable RNA replication1 23 . The SARS-CoV-2 RO is composed of double membrane 24 vesicles (DMVs) tethered to the endoplasmic reticulum (ER) by thin membrane connectors2 25 , but the viral proteins and the host factors involved are currently unknown. 26 Here we identify the viral non-structural proteins (NSPs) that generate the SARS-CoV-2 27 RO. NSP3 and NSP4 generate the DMVs while NSP6, through oligomerization and an 28 amphipathic helix, zippers ER membranes and establishes the connectors. The 29 NSP6ΔSGF mutant, which arose independently in the α, β, γ, η, ι, and λ variants of SARS30 CoV-2, behaves as a gain-of-function mutant with a higher ER-zippering activity. We 31 identified three main roles for NSP6: to act as a filter in RO-ER communication allowing 32 lipid flow but restricting access of ER luminal proteins to the DMVs, to position and 33 organize DMV clusters, and to mediate contact with lipid droplets (LDs) via the LD34 tethering complex DFCP1-Rab18. NSP6 thus acts as an organizer of DMV clusters and can 35 provide a selective track to refurbish them with LD-derived lipids. Importantly, both 36 properly formed NSP6 connectors and LDs are required for SARS-CoV-2 replication. Our 37 findings, uncovering the biological activity of NSP6 of SARS-CoV-2 and of other 38 coronaviruses, have the potential to fuel the search for broad antiviral agents.engNatureBiogênesePapel da NSP6OrganellaReplicação do SARS-cOV2Role of NSP6BiogenesisReplication organelleSARS-CoV-2The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALViniciusSoares_SuelenDias_PatriciaBozza_etal_IOC_2022.pdfViniciusSoares_SuelenDias_PatriciaBozza_etal_IOC_2022.pdfapplication/pdf79154469https://www.arca.fiocruz.br/bitstream/icict/53156/2/ViniciusSoares_SuelenDias_PatriciaBozza_etal_IOC_2022.pdf3d897089d71d6aba7aec1923661422e4MD52LICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle
title The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle
spellingShingle The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle
Ricciardi, Simona
Biogênese
Papel da NSP6
Organella
Replicação do SARS-cOV2
Role of NSP6
Biogenesis
Replication organelle
SARS-CoV-2
title_short The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle
title_full The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle
title_fullStr The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle
title_full_unstemmed The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle
title_sort The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle
author Ricciardi, Simona
author_facet Ricciardi, Simona
Guarino, Andrea Maria
Giaquinto, Laura
Polishchuk, Elena V.
Santoro, Michele
Di Tulio, Giuseppe
Wilson, Cathal
Panariello, Francesco
Soares, Vinicius C.
Dias, Suelen S. G.
Santos, Julia C.
Souza, Thiago Moreno L.
Fusco, Giovanna
Viscardi, Maurizio
Brandi, Sergio
Bozza, Patrícia Torres
Polishchuk, Roman S.
Venditti, Rossella
Matteis, Maria Antonieta De
author_role author
author2 Guarino, Andrea Maria
Giaquinto, Laura
Polishchuk, Elena V.
Santoro, Michele
Di Tulio, Giuseppe
Wilson, Cathal
Panariello, Francesco
Soares, Vinicius C.
Dias, Suelen S. G.
Santos, Julia C.
Souza, Thiago Moreno L.
Fusco, Giovanna
Viscardi, Maurizio
Brandi, Sergio
Bozza, Patrícia Torres
Polishchuk, Roman S.
Venditti, Rossella
Matteis, Maria Antonieta De
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ricciardi, Simona
Guarino, Andrea Maria
Giaquinto, Laura
Polishchuk, Elena V.
Santoro, Michele
Di Tulio, Giuseppe
Wilson, Cathal
Panariello, Francesco
Soares, Vinicius C.
Dias, Suelen S. G.
Santos, Julia C.
Souza, Thiago Moreno L.
Fusco, Giovanna
Viscardi, Maurizio
Brandi, Sergio
Bozza, Patrícia Torres
Polishchuk, Roman S.
Venditti, Rossella
Matteis, Maria Antonieta De
dc.subject.other.pt_BR.fl_str_mv Biogênese
Papel da NSP6
Organella
Replicação do SARS-cOV2
topic Biogênese
Papel da NSP6
Organella
Replicação do SARS-cOV2
Role of NSP6
Biogenesis
Replication organelle
SARS-CoV-2
dc.subject.en.pt_BR.fl_str_mv Role of NSP6
Biogenesis
Replication organelle
SARS-CoV-2
description This is a PDF file of a peer-reviewed paper that has been accepted for publication. Although unedited, the content has been subjected to preliminary formatting. Nature is providing this early version of the typeset paper as a service to our authors and readers. The text and figures will undergo copyediting and a proof review before the paper is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers apply.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-06-09T18:48:25Z
dc.date.available.fl_str_mv 2022-06-09T18:48:25Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RICCIARDI, Simona et al. The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle. Nature, p. 1-42, May 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/53156
dc.identifier.issn.pt_BR.fl_str_mv 1476-4687
dc.identifier.doi.pt_BR.fl_str_mv 10.1038/s41586-022-04835-6
identifier_str_mv RICCIARDI, Simona et al. The role of NSP6 in the biogenesis of the SARS-CoV-2 replication organelle. Nature, p. 1-42, May 2022.
1476-4687
10.1038/s41586-022-04835-6
url https://www.arca.fiocruz.br/handle/icict/53156
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature
publisher.none.fl_str_mv Nature
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/53156/2/ViniciusSoares_SuelenDias_PatriciaBozza_etal_IOC_2022.pdf
https://www.arca.fiocruz.br/bitstream/icict/53156/3/license.txt
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