Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine

Detalhes bibliográficos
Autor(a) principal: Matos, Denise Cristina Souza
Data de Publicação: 2012
Outros Autores: Marcovistz, Rugimar, Silva, Andréa Marques Vieira da, Quintilio, Wagner, Georgini, Ricardo Amaral
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32005
Resumo: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
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spelling Matos, Denise Cristina SouzaMarcovistz, RugimarSilva, Andréa Marques Vieira daQuintilio, WagnerGeorgini, Ricardo Amaral2019-03-08T17:18:28Z2019-03-08T17:18:28Z2012MATOS, Denise Cristina Souza et al. Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine. Braz. J. Microbiol., São Paulo , v. 43, n. 2, p. 429-431, 2012.1678-4405https://www.arca.fiocruz.br/handle/icict/3200510.1590/S1517-83822012000200001engDTP vaccinePertussis whole cellEnzyme-linked immunosorbent assayIntracerebral mouse protection testComparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Instituto Butantan. São Paulo, SP, Brasil.Instituto Butantan. São Paulo, SP, Brasil.The aim of this study was to compare the PSPT standardized in-house as an alternative to MPT for potency assays of pertussis component. Statistical analyses have showed similar pertussis potency values when PSPT was compared to MPT. Significant correlation between the potency results obtained by in vivo and in vitro assays was also been observed. Results by PSPT have demonstrated reproducibility and accuracy for potency pertussis control and this approach has been considered promising for use at least during the steps of production.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/32005/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALF84-bjm-43-429.pdfapplication/pdf143463https://www.arca.fiocruz.br/bitstream/icict/32005/2/F84-bjm-43-429.pdf6265b56448265bcbf69acc34925dc796MD52TEXTF84-bjm-43-429.pdf.txtF84-bjm-43-429.pdf.txtExtracted texttext/plain11335https://www.arca.fiocruz.br/bitstream/icict/32005/3/F84-bjm-43-429.pdf.txtbaa0a480eaaa31274980c11b19b29a50MD53icict/320052019-04-04 02:05:06.035oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-04-04T05:05:06Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
title Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
spellingShingle Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
Matos, Denise Cristina Souza
DTP vaccine
Pertussis whole cell
Enzyme-linked immunosorbent assay
Intracerebral mouse protection test
title_short Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
title_full Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
title_fullStr Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
title_full_unstemmed Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
title_sort Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine
author Matos, Denise Cristina Souza
author_facet Matos, Denise Cristina Souza
Marcovistz, Rugimar
Silva, Andréa Marques Vieira da
Quintilio, Wagner
Georgini, Ricardo Amaral
author_role author
author2 Marcovistz, Rugimar
Silva, Andréa Marques Vieira da
Quintilio, Wagner
Georgini, Ricardo Amaral
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Matos, Denise Cristina Souza
Marcovistz, Rugimar
Silva, Andréa Marques Vieira da
Quintilio, Wagner
Georgini, Ricardo Amaral
dc.subject.other.pt_BR.fl_str_mv DTP vaccine
Pertussis whole cell
Enzyme-linked immunosorbent assay
Intracerebral mouse protection test
topic DTP vaccine
Pertussis whole cell
Enzyme-linked immunosorbent assay
Intracerebral mouse protection test
description Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2019-03-08T17:18:28Z
dc.date.available.fl_str_mv 2019-03-08T17:18:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MATOS, Denise Cristina Souza et al. Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine. Braz. J. Microbiol., São Paulo , v. 43, n. 2, p. 429-431, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32005
dc.identifier.issn.pt_BR.fl_str_mv 1678-4405
dc.identifier.doi.none.fl_str_mv 10.1590/S1517-83822012000200001
identifier_str_mv MATOS, Denise Cristina Souza et al. Comparative analysis of the intracerebral mouse protection test and serological method for potency assays of pertussis component in DTP vaccine. Braz. J. Microbiol., São Paulo , v. 43, n. 2, p. 429-431, 2012.
1678-4405
10.1590/S1517-83822012000200001
url https://www.arca.fiocruz.br/handle/icict/32005
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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