Neutrophil paralysis in Plasmodium vivax malaria

Detalhes bibliográficos
Autor(a) principal: Leoratti, Fabiana Maria de Souza
Data de Publicação: 2012
Outros Autores: Trevelin, Silvia Cellone, Cunha, Fernando Queiroz, Rocha, Bruno Coelho, Costa, Pedro Augusto Carvalho, Gravina, Humberto Doriguêtto, Tada, Mauro Shugiro, Pereira, Dhelio Batista, Golenbock, Douglas Taylor, Antonelli, Lis Ribeiro do Valle, Gazzinelli, Ricardo Tostes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7635
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil
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spelling Leoratti, Fabiana Maria de SouzaTrevelin, Silvia CelloneCunha, Fernando QueirozRocha, Bruno CoelhoCosta, Pedro Augusto CarvalhoGravina, Humberto DoriguêttoTada, Mauro ShugiroPereira, Dhelio BatistaGolenbock, Douglas TaylorAntonelli, Lis Ribeiro do ValleGazzinelli, Ricardo Tostes2014-05-22T12:55:03Z2014-05-22T12:55:03Z2012LEORATTI, Fabiana Maria de Souza et al. Neutrophil paralysis in Plasmodium vivax malaria. Plos Neglected Tropical Diseases. 2012, vol.6, pp. e17101935-2735https://www.arca.fiocruz.br/handle/icict/763510.1371/journal.pntd.0001710engPublic Library of ScienceNeutrophil paralysis in Plasmodium vivax malariainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/ Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/ Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilCentro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, BrazilCentro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/University of Massachusetts. Medical School. Division of Infectious Diseases and Immunology. Worcester, MA, United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/University of Massachusetts. Medical School. Division of Infectious Diseases and Immunology. Worcester, MA, United States of America/ Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilBackground The activation of innate immune responses by Plasmodium vivax results in activation of effector cells and an excessive production of pro-inflammatory cytokines that may culminate in deleterious effects. Here, we examined the activation and function of neutrophils during acute episodes of malaria. Materials and Methods Blood samples were collected from P. vivax-infected patients at admission (day 0) and 30–45 days after treatment with chloroquine and primaquine. Expression of activation markers and cytokine levels produced by highly purified monocytes and neutrophils were measured by the Cytometric Bead Assay. Phagocytic activity, superoxide production, chemotaxis and the presence of G protein-coupled receptor (GRK2) were also evaluated in neutrophils from malaria patients. Principal Findings Both monocytes and neutrophils from P. vivax-infected patients were highly activated. While monocytes were found to be the main source of cytokines in response to TLR ligands, neutrophils showed enhanced phagocytic activity and superoxide production. Interestingly, neutrophils from the malaria patients expressed high levels of GRK2, low levels of CXCR2, and displayed impaired chemotaxis towards IL-8 (CXCL8). Conclusion Activated neutrophils from malaria patients are a poor source of pro-inflammatory cytokines and display reduced chemotactic activity, suggesting a possible mechanism for an enhanced susceptibility to secondary bacterial infection during malaria.Plasmodium vivaxMalariaImmune responseNeutrophilsG protein-coupled receptor kinasesChemotaxisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALNeutrophil paralysis in Plasmodium vivax malaria.pdfNeutrophil paralysis in Plasmodium vivax malaria.pdfapplication/pdf1522928https://www.arca.fiocruz.br/bitstream/icict/7635/1/Neutrophil%20paralysis%20in%20Plasmodium%20vivax%20malaria.pdf75ff47c75d5e18d24cff129b1244fb72MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7635/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTNeutrophil paralysis in Plasmodium vivax malaria.pdf.txtNeutrophil paralysis in Plasmodium vivax malaria.pdf.txtExtracted texttext/plain9https://www.arca.fiocruz.br/bitstream/icict/7635/5/Neutrophil%20paralysis%20in%20Plasmodium%20vivax%20malaria.pdf.txt33f4f15a16a9843faf6a25d4f387b6fdMD55THUMBNAILNeutrophil paralysis in Plasmodium vivax malaria.pdf.jpgNeutrophil paralysis in Plasmodium vivax malaria.pdf.jpgGenerated Thumbnailimage/jpeg2271https://www.arca.fiocruz.br/bitstream/icict/7635/4/Neutrophil%20paralysis%20in%20Plasmodium%20vivax%20malaria.pdf.jpg38c80b7d4bc756e562faa47c32f95584MD54icict/76352018-04-06 08:43:57.648oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:43:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Neutrophil paralysis in Plasmodium vivax malaria
title Neutrophil paralysis in Plasmodium vivax malaria
spellingShingle Neutrophil paralysis in Plasmodium vivax malaria
Leoratti, Fabiana Maria de Souza
Plasmodium vivax
Malaria
Immune response
Neutrophils
G protein-coupled receptor kinases
Chemotaxis
title_short Neutrophil paralysis in Plasmodium vivax malaria
title_full Neutrophil paralysis in Plasmodium vivax malaria
title_fullStr Neutrophil paralysis in Plasmodium vivax malaria
title_full_unstemmed Neutrophil paralysis in Plasmodium vivax malaria
title_sort Neutrophil paralysis in Plasmodium vivax malaria
author Leoratti, Fabiana Maria de Souza
author_facet Leoratti, Fabiana Maria de Souza
Trevelin, Silvia Cellone
Cunha, Fernando Queiroz
Rocha, Bruno Coelho
Costa, Pedro Augusto Carvalho
Gravina, Humberto Doriguêtto
Tada, Mauro Shugiro
Pereira, Dhelio Batista
Golenbock, Douglas Taylor
Antonelli, Lis Ribeiro do Valle
Gazzinelli, Ricardo Tostes
author_role author
author2 Trevelin, Silvia Cellone
Cunha, Fernando Queiroz
Rocha, Bruno Coelho
Costa, Pedro Augusto Carvalho
Gravina, Humberto Doriguêtto
Tada, Mauro Shugiro
Pereira, Dhelio Batista
Golenbock, Douglas Taylor
Antonelli, Lis Ribeiro do Valle
Gazzinelli, Ricardo Tostes
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Leoratti, Fabiana Maria de Souza
Trevelin, Silvia Cellone
Cunha, Fernando Queiroz
Rocha, Bruno Coelho
Costa, Pedro Augusto Carvalho
Gravina, Humberto Doriguêtto
Tada, Mauro Shugiro
Pereira, Dhelio Batista
Golenbock, Douglas Taylor
Antonelli, Lis Ribeiro do Valle
Gazzinelli, Ricardo Tostes
dc.subject.en.pt_BR.fl_str_mv Plasmodium vivax
Malaria
Immune response
Neutrophils
G protein-coupled receptor kinases
Chemotaxis
topic Plasmodium vivax
Malaria
Immune response
Neutrophils
G protein-coupled receptor kinases
Chemotaxis
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-05-22T12:55:03Z
dc.date.available.fl_str_mv 2014-05-22T12:55:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv LEORATTI, Fabiana Maria de Souza et al. Neutrophil paralysis in Plasmodium vivax malaria. Plos Neglected Tropical Diseases. 2012, vol.6, pp. e1710
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7635
dc.identifier.issn.none.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0001710
identifier_str_mv LEORATTI, Fabiana Maria de Souza et al. Neutrophil paralysis in Plasmodium vivax malaria. Plos Neglected Tropical Diseases. 2012, vol.6, pp. e1710
1935-2735
10.1371/journal.pntd.0001710
url https://www.arca.fiocruz.br/handle/icict/7635
dc.language.iso.fl_str_mv eng
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