Neutrophil paralysis in Plasmodium vivax malaria
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/7635 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil |
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Leoratti, Fabiana Maria de SouzaTrevelin, Silvia CelloneCunha, Fernando QueirozRocha, Bruno CoelhoCosta, Pedro Augusto CarvalhoGravina, Humberto DoriguêttoTada, Mauro ShugiroPereira, Dhelio BatistaGolenbock, Douglas TaylorAntonelli, Lis Ribeiro do ValleGazzinelli, Ricardo Tostes2014-05-22T12:55:03Z2014-05-22T12:55:03Z2012LEORATTI, Fabiana Maria de Souza et al. Neutrophil paralysis in Plasmodium vivax malaria. Plos Neglected Tropical Diseases. 2012, vol.6, pp. e17101935-2735https://www.arca.fiocruz.br/handle/icict/763510.1371/journal.pntd.0001710engPublic Library of ScienceNeutrophil paralysis in Plasmodium vivax malariainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/ Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/ Universidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Farmacologia. Ribeirão Preto, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilCentro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, BrazilCentro de Pesquisas em Medicina Tropical de Rondônia. Porto Velho, RO, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/University of Massachusetts. Medical School. Division of Infectious Diseases and Immunology. Worcester, MA, United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil/University of Massachusetts. Medical School. Division of Infectious Diseases and Immunology. Worcester, MA, United States of America/ Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilBackground The activation of innate immune responses by Plasmodium vivax results in activation of effector cells and an excessive production of pro-inflammatory cytokines that may culminate in deleterious effects. Here, we examined the activation and function of neutrophils during acute episodes of malaria. Materials and Methods Blood samples were collected from P. vivax-infected patients at admission (day 0) and 30–45 days after treatment with chloroquine and primaquine. Expression of activation markers and cytokine levels produced by highly purified monocytes and neutrophils were measured by the Cytometric Bead Assay. Phagocytic activity, superoxide production, chemotaxis and the presence of G protein-coupled receptor (GRK2) were also evaluated in neutrophils from malaria patients. Principal Findings Both monocytes and neutrophils from P. vivax-infected patients were highly activated. While monocytes were found to be the main source of cytokines in response to TLR ligands, neutrophils showed enhanced phagocytic activity and superoxide production. Interestingly, neutrophils from the malaria patients expressed high levels of GRK2, low levels of CXCR2, and displayed impaired chemotaxis towards IL-8 (CXCL8). Conclusion Activated neutrophils from malaria patients are a poor source of pro-inflammatory cytokines and display reduced chemotactic activity, suggesting a possible mechanism for an enhanced susceptibility to secondary bacterial infection during malaria.Plasmodium vivaxMalariaImmune responseNeutrophilsG protein-coupled receptor kinasesChemotaxisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALNeutrophil paralysis in Plasmodium vivax malaria.pdfNeutrophil paralysis in Plasmodium vivax malaria.pdfapplication/pdf1522928https://www.arca.fiocruz.br/bitstream/icict/7635/1/Neutrophil%20paralysis%20in%20Plasmodium%20vivax%20malaria.pdf75ff47c75d5e18d24cff129b1244fb72MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7635/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTNeutrophil paralysis in Plasmodium vivax malaria.pdf.txtNeutrophil paralysis in Plasmodium vivax malaria.pdf.txtExtracted texttext/plain9https://www.arca.fiocruz.br/bitstream/icict/7635/5/Neutrophil%20paralysis%20in%20Plasmodium%20vivax%20malaria.pdf.txt33f4f15a16a9843faf6a25d4f387b6fdMD55THUMBNAILNeutrophil paralysis in Plasmodium vivax malaria.pdf.jpgNeutrophil paralysis in Plasmodium vivax malaria.pdf.jpgGenerated Thumbnailimage/jpeg2271https://www.arca.fiocruz.br/bitstream/icict/7635/4/Neutrophil%20paralysis%20in%20Plasmodium%20vivax%20malaria.pdf.jpg38c80b7d4bc756e562faa47c32f95584MD54icict/76352018-04-06 08:43:57.648oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:43:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Neutrophil paralysis in Plasmodium vivax malaria |
| title |
Neutrophil paralysis in Plasmodium vivax malaria |
| spellingShingle |
Neutrophil paralysis in Plasmodium vivax malaria Leoratti, Fabiana Maria de Souza Plasmodium vivax Malaria Immune response Neutrophils G protein-coupled receptor kinases Chemotaxis |
| title_short |
Neutrophil paralysis in Plasmodium vivax malaria |
| title_full |
Neutrophil paralysis in Plasmodium vivax malaria |
| title_fullStr |
Neutrophil paralysis in Plasmodium vivax malaria |
| title_full_unstemmed |
Neutrophil paralysis in Plasmodium vivax malaria |
| title_sort |
Neutrophil paralysis in Plasmodium vivax malaria |
| author |
Leoratti, Fabiana Maria de Souza |
| author_facet |
Leoratti, Fabiana Maria de Souza Trevelin, Silvia Cellone Cunha, Fernando Queiroz Rocha, Bruno Coelho Costa, Pedro Augusto Carvalho Gravina, Humberto Doriguêtto Tada, Mauro Shugiro Pereira, Dhelio Batista Golenbock, Douglas Taylor Antonelli, Lis Ribeiro do Valle Gazzinelli, Ricardo Tostes |
| author_role |
author |
| author2 |
Trevelin, Silvia Cellone Cunha, Fernando Queiroz Rocha, Bruno Coelho Costa, Pedro Augusto Carvalho Gravina, Humberto Doriguêtto Tada, Mauro Shugiro Pereira, Dhelio Batista Golenbock, Douglas Taylor Antonelli, Lis Ribeiro do Valle Gazzinelli, Ricardo Tostes |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Leoratti, Fabiana Maria de Souza Trevelin, Silvia Cellone Cunha, Fernando Queiroz Rocha, Bruno Coelho Costa, Pedro Augusto Carvalho Gravina, Humberto Doriguêtto Tada, Mauro Shugiro Pereira, Dhelio Batista Golenbock, Douglas Taylor Antonelli, Lis Ribeiro do Valle Gazzinelli, Ricardo Tostes |
| dc.subject.en.pt_BR.fl_str_mv |
Plasmodium vivax Malaria Immune response Neutrophils G protein-coupled receptor kinases Chemotaxis |
| topic |
Plasmodium vivax Malaria Immune response Neutrophils G protein-coupled receptor kinases Chemotaxis |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunopatologia. Belo Horizonte, MG, Brazil |
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2012 |
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2012 |
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2014-05-22T12:55:03Z |
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2014-05-22T12:55:03Z |
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LEORATTI, Fabiana Maria de Souza et al. Neutrophil paralysis in Plasmodium vivax malaria. Plos Neglected Tropical Diseases. 2012, vol.6, pp. e1710 |
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https://www.arca.fiocruz.br/handle/icict/7635 |
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1935-2735 |
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10.1371/journal.pntd.0001710 |
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LEORATTI, Fabiana Maria de Souza et al. Neutrophil paralysis in Plasmodium vivax malaria. Plos Neglected Tropical Diseases. 2012, vol.6, pp. e1710 1935-2735 10.1371/journal.pntd.0001710 |
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