Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages.

Ibraim, Izabela Coimbra; Assis, Rafael Ramiro de; Pessoa, Natália Lima; Campos, Marco Antônio; Melo, Maria Norma; Turco, Salvatore Joseph; Soares, Rodrigo Pedro Pinto

Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages.

Detalhes bibliográficos
Autor(a) principal:	Ibraim, Izabela Coimbra
Data de Publicação:	2013
Outros Autores:	Assis, Rafael Ramiro de, Pessoa, Natália Lima, Campos, Marco Antônio, Melo, Maria Norma, Turco, Salvatore Joseph, Soares, Rodrigo Pedro Pinto
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/30196
Resumo:	2020-01-01

Metadados do item

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spelling	Ibraim, Izabela CoimbraAssis, Rafael Ramiro dePessoa, Natália LimaCampos, Marco AntônioMelo, Maria NormaTurco, Salvatore JosephSoares, Rodrigo Pedro Pinto2018-11-26T16:22:55Z2018-11-26T16:22:55Z2013IBRAIM, Izabela Coimbra et al. Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages. Parasit Vectors., v; 6, p. 54, 20131756-3305https://www.arca.fiocruz.br/handle/icict/3019610.1186/1756-3305-6-54engBioMed CentralLeishmania infantumLeishmania braziliensisLipofosfoglicano (LPG)Modulação de macrófagosLeishmania infantumLeishmania braziliensisLipophosphoglycan (LPG)Macrophage modulationTwo biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-01-01Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilDepartment of Biochemistry. University of Kentucky Medical Center. Lexington, KY, USAFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilBACKGROUND: The dominant, cell surface lipophosphoglycan (LPG) of Leishmania is a multifunctional molecule involved in the interaction with vertebrate and invertebrate hosts. Although the role of LPG on infection has been extensively studied, it is not known if LPG interspecies variations contribute to the different immunopathologies of leishmaniases. To investigate the issue of interspecies polymorphisms, two Leishmania species from the New World that express structural variations of side chains of LPG repeat units were examined. In this context, the procyclic form of L. braziliensis LPG (strain M2903), is devoid of side chains, while the L. infantum LPG (strain BH46) has up to three glucoses residues in the repeat units. METHODS: Mice peritoneal macrophages from Balb/c, C57BL/6 and knock-out (TLR2 -/-, TLR4 -/-) were primed with IFN-γ and stimulated with purified LPG from both species. Nitric oxide and cytokine production, MAPKs (ERK, p38 and JNK) and NF-kB activation were evaluated. RESULTS: Macrophages stimulated with L. braziliensis LPG, had a higher TNF-α, IL-1β, IL-6 and NO production than those stimulated with that of L. infantum. Furthermore, the LPGs from the two species resulted in differential kinetics of signaling via MAPK activation. L. infantum LPG exhibited a gradual activation profile, whereas L. braziliensis LPG showed a sharp but transient activation. L. braziliensis LPG was able to activate NF-kB. CONCLUSION: These data suggest that two biochemically distinct LPGs were able to differentially modulate macrophage functionsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30196/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALTwo biochemically distinct lipophosphoglycans.pdfTwo biochemically distinct lipophosphoglycans.pdfapplication/pdf11130537https://www.arca.fiocruz.br/bitstream/icict/30196/2/Two%20biochemically%20distinct%20lipophosphoglycans.pdf04d06e61b974ecbc551e0d2bcaac307aMD52TEXTTwo biochemically distinct lipophosphoglycans.pdf.txtTwo biochemically distinct lipophosphoglycans.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv	Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages.
title	Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages.
spellingShingle	Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages. Ibraim, Izabela Coimbra Leishmania infantum Leishmania braziliensis Lipofosfoglicano (LPG) Modulação de macrófagos Leishmania infantum Leishmania braziliensis Lipophosphoglycan (LPG) Macrophage modulation
title_short	Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages.
title_full	Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages.
title_fullStr	Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages.
title_full_unstemmed	Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages.
title_sort	Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages.
author	Ibraim, Izabela Coimbra
author_facet	Ibraim, Izabela Coimbra Assis, Rafael Ramiro de Pessoa, Natália Lima Campos, Marco Antônio Melo, Maria Norma Turco, Salvatore Joseph Soares, Rodrigo Pedro Pinto
author_role	author
author2	Assis, Rafael Ramiro de Pessoa, Natália Lima Campos, Marco Antônio Melo, Maria Norma Turco, Salvatore Joseph Soares, Rodrigo Pedro Pinto
author2_role	author author author author author author
dc.contributor.author.fl_str_mv	Ibraim, Izabela Coimbra Assis, Rafael Ramiro de Pessoa, Natália Lima Campos, Marco Antônio Melo, Maria Norma Turco, Salvatore Joseph Soares, Rodrigo Pedro Pinto
dc.subject.other.pt_BR.fl_str_mv	Leishmania infantum Leishmania braziliensis Lipofosfoglicano (LPG) Modulação de macrófagos
topic	Leishmania infantum Leishmania braziliensis Lipofosfoglicano (LPG) Modulação de macrófagos Leishmania infantum Leishmania braziliensis Lipophosphoglycan (LPG) Macrophage modulation
dc.subject.en.pt_BR.fl_str_mv	Leishmania infantum Leishmania braziliensis Lipophosphoglycan (LPG) Macrophage modulation
description	2020-01-01
publishDate	2013
dc.date.issued.fl_str_mv	2013
dc.date.accessioned.fl_str_mv	2018-11-26T16:22:55Z
dc.date.available.fl_str_mv	2018-11-26T16:22:55Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	IBRAIM, Izabela Coimbra et al. Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages. Parasit Vectors., v; 6, p. 54, 2013
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/30196
dc.identifier.issn.pt_BR.fl_str_mv	1756-3305
dc.identifier.doi.none.fl_str_mv	10.1186/1756-3305-6-54
identifier_str_mv	IBRAIM, Izabela Coimbra et al. Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages. Parasit Vectors., v; 6, p. 54, 2013 1756-3305 10.1186/1756-3305-6-54
url	https://www.arca.fiocruz.br/handle/icict/30196
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	BioMed Central
publisher.none.fl_str_mv	BioMed Central
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv	https://www.arca.fiocruz.br/bitstream/icict/30196/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/30196/2/Two%20biochemically%20distinct%20lipophosphoglycans.pdf https://www.arca.fiocruz.br/bitstream/icict/30196/3/Two%20biochemically%20distinct%20lipophosphoglycans.pdf.txt
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repository.name.fl_str_mv	Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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Two biochemically distinct lipophosphoglycans from Leishmania braziliensis and Leishmania infantum trigger different innate immune responses in murine macrophages.

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