Leishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophils
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.3389/fcimb.2022.788196 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/52565 |
Resumo: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Programa Inova – Geração de Conhecimentos. Institutos Canadenses de Pesquisa em Saúde. Coordenação de Aperfeiçoamento de Pessoal de Nı́vel Superior (CAPES). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). |
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Carvalho, Graziele QuintelaGoicochea, Astrid Madeleine CaleroSantos, Vanessa MançurViana, Sayonara de MeloLuz, Yasmin da SilvaDias, Beatriz Rocha SimõesSouza, Milena LázaroSuarez, MarthaOliveira, Camila Indiani deSaraiva, Elvira M.Brodskyn, Cláudia I.Veras, Patrícia T.Menezes, Juliana P. B. deAndrade, Bruno B.Lima, Jonilson BerlinkDescoteaux, AlbertBorges, Valéria M.2022-05-06T16:48:32Z2022-05-06T16:48:32Z2022CARVALHO, Graziele Quintela et al. Leishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophils. Frontiers in Cellular and Infection Microbiology, v. 11, p. 1-12, 2022.2235-2988https://www.arca.fiocruz.br/handle/icict/5256510.3389/fcimb.2022.788196Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Programa Inova – Geração de Conhecimentos. Institutos Canadenses de Pesquisa em Saúde. Coordenação de Aperfeiçoamento de Pessoal de Nı́vel Superior (CAPES). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Instituto Federal de Educação, Ciência e Tecnologia Baiano. Alagoinhas, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Departamento de Imunologia. Laboratório de Imunobiologia das Leishmanioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Iniciativa de Pesquisa Translacional e Epidemiológica da Rede de Organização Multinacional (MONSTER). Salvador, BA, Brasil / Faculdade de Tecnologia e Ciências. Curso de Medicina. Salvador, BA, Brasil / Universidade Salvador. Laureate Universities. Salvador, BA, Brasil.Universidade Federal do Oeste da Bahia. Núcleo de Agentes Infecciosos e Vetores. Barreiras, BA, Brasil.Institut National de la Recherche Scientifique. Centre Armand-Frappier Santé Biotechnologie. Laval, QC, Canada.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil.Visceral leishmaniasis (VL) is often associated with hematologic manifestations that may interfere with neutrophil response. Lipophosphoglycan (LPG) is a major molecule on the surface of Leishmania promastigotes, which has been associated with several aspects of the parasite–vector–host interplay. Here, we investigated how LPG from Leishmania (L.) infantum, the principal etiological agent of VL in the New World, influences the initial establishment of infection during interaction with human neutrophils in an experimental setting in vitro. Human neutrophils obtained from peripheral blood samples were infected with either the wild-type L. infantum (WT) strain or LPG-deficient mutant (∆lpg1). In this setting, ∆lpg1 parasites displayed reduced viability compared to WT L. infantum; such finding was reverted in the complemented ∆lpg1+LPG1 parasites at 3- and 6-h post-infection. Confocal microscopy experiments indicated that this decreased survival was related to enhanced lysosomal fusion. In fact, LPG-deficient L. infantum parasites more frequently died inside neutrophil acidic compartments, a phenomenon that was reverted when host cells were treated with Wortmannin. We also observed an increase in the secretion of the neutrophil collagenase matrix metalloproteinase-8 (MMP-8) by cells infected with ∆lpg1 L. infantum compared to those that were infected with WT parasites. Furthermore, collagen I matrix degradation was found to be significantly increased in ∆lpg1 parasite-infected cells but not in WT-infected controls. Flow cytometry analysis revealed a substantial boost in production of reactive oxygen species (ROS) during infection with either WT or ∆lpg1 L. infantum. In addition, killing of ∆lpg1 parasites was shown to be more dependent on the ROS production than that of WT L. infantum. Notably, inhibition of the oxidative stress with Apocynin potentially fueled ∆lpg1 L. infantum fitness as it increased the intracellular parasite viability. Thus, our observations demonstrate that LPG may be a critical molecule fostering parasite survival in human neutrophils through a mechanism that involves cellular activation and generation of free radicals.engFrontiers MediaLipofosfoglicanoLeishmania infantumNeutrófilosROSInfecçãoLipophosphoglycanLeishmania infantumNeutrophilsROSInfectionLeishmania infantumNeutrófilosInfecçõesLeishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophilsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/52565/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCarvalho, G. Q. - Leishmania infantum Defective.pdfCarvalho, G. Q. - Leishmania infantum Defective.pdfapplication/pdf4670721https://www.arca.fiocruz.br/bitstream/icict/52565/2/Carvalho%2c%20G.%20Q.%20-%20Leishmania%20infantum%20Defective.pdf463aed0dd8de7ebdbba9e215287a17d2MD52icict/525652023-03-15 14:34:34.557oai:www.arca.fiocruz.br:icict/52565Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:34Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Leishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophils |
| title |
Leishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophils |
| spellingShingle |
Leishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophils Carvalho, Graziele Quintela Lipofosfoglicano Leishmania infantum Neutrófilos ROS Infecção Lipophosphoglycan Leishmania infantum Neutrophils ROS Infection Leishmania infantum Neutrófilos Infecções |
| title_short |
Leishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophils |
| title_full |
Leishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophils |
| title_fullStr |
Leishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophils |
| title_full_unstemmed |
Leishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophils |
| title_sort |
Leishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophils |
| author |
Carvalho, Graziele Quintela |
| author_facet |
Carvalho, Graziele Quintela Goicochea, Astrid Madeleine Calero Santos, Vanessa Mançur Viana, Sayonara de Melo Luz, Yasmin da Silva Dias, Beatriz Rocha Simões Souza, Milena Lázaro Suarez, Martha Oliveira, Camila Indiani de Saraiva, Elvira M. Brodskyn, Cláudia I. Veras, Patrícia T. Menezes, Juliana P. B. de Andrade, Bruno B. Lima, Jonilson Berlink Descoteaux, Albert Borges, Valéria M. |
| author_role |
author |
| author2 |
Goicochea, Astrid Madeleine Calero Santos, Vanessa Mançur Viana, Sayonara de Melo Luz, Yasmin da Silva Dias, Beatriz Rocha Simões Souza, Milena Lázaro Suarez, Martha Oliveira, Camila Indiani de Saraiva, Elvira M. Brodskyn, Cláudia I. Veras, Patrícia T. Menezes, Juliana P. B. de Andrade, Bruno B. Lima, Jonilson Berlink Descoteaux, Albert Borges, Valéria M. |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Carvalho, Graziele Quintela Goicochea, Astrid Madeleine Calero Santos, Vanessa Mançur Viana, Sayonara de Melo Luz, Yasmin da Silva Dias, Beatriz Rocha Simões Souza, Milena Lázaro Suarez, Martha Oliveira, Camila Indiani de Saraiva, Elvira M. Brodskyn, Cláudia I. Veras, Patrícia T. Menezes, Juliana P. B. de Andrade, Bruno B. Lima, Jonilson Berlink Descoteaux, Albert Borges, Valéria M. |
| dc.subject.other.pt_BR.fl_str_mv |
Lipofosfoglicano Leishmania infantum Neutrófilos ROS Infecção |
| topic |
Lipofosfoglicano Leishmania infantum Neutrófilos ROS Infecção Lipophosphoglycan Leishmania infantum Neutrophils ROS Infection Leishmania infantum Neutrófilos Infecções |
| dc.subject.en.pt_BR.fl_str_mv |
Lipophosphoglycan Leishmania infantum Neutrophils ROS Infection |
| dc.subject.decs.pt_BR.fl_str_mv |
Leishmania infantum Neutrófilos Infecções |
| description |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). Programa Inova – Geração de Conhecimentos. Institutos Canadenses de Pesquisa em Saúde. Coordenação de Aperfeiçoamento de Pessoal de Nı́vel Superior (CAPES). Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). |
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2022 |
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2022-05-06T16:48:32Z |
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2022-05-06T16:48:32Z |
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2022 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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CARVALHO, Graziele Quintela et al. Leishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophils. Frontiers in Cellular and Infection Microbiology, v. 11, p. 1-12, 2022. |
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https://www.arca.fiocruz.br/handle/icict/52565 |
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2235-2988 |
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10.3389/fcimb.2022.788196 |
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CARVALHO, Graziele Quintela et al. Leishmania infantum defective in lipophosphoglycan biosynthesis interferes with activation of human neutrophils. Frontiers in Cellular and Infection Microbiology, v. 11, p. 1-12, 2022. 2235-2988 10.3389/fcimb.2022.788196 |
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https://www.arca.fiocruz.br/handle/icict/52565 |
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eng |
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eng |
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Frontiers Media |
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Frontiers Media |
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