Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation

Detalhes bibliográficos
Autor(a) principal: Messias, Carolina V.
Data de Publicação: 2016
Outros Autores: Van-Vliet, Eliane Santana, Lemos, Julia P., Moreira, Otacilio C., Almeida, Vinicius Cotta de, Savino, Wilson, Cruz, Daniella Arêas Mendes da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16599
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.
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spelling Messias, Carolina V.Van-Vliet, Eliane SantanaLemos, Julia P.Moreira, Otacilio C.Almeida, Vinicius Cotta deSavino, WilsonCruz, Daniella Arêas Mendes da2016-12-29T14:17:22Z2016-12-29T14:17:22Z2016MESSIAS, Carolina V. et al. Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation. Plos One, v.11, n.1, e0148137, 20p, Jan. 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1659910.1371/journal.pone.0148137engPublic Library of ScienceQuimiotaxiaBlastosS1P1Sphingosine-1-phosphate(S1P)ChemotaxisT-ALL BlastsFugetaxisS1P1 ActivationSphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid involved in several physiological processes including cell migration and differentiation. S1P signaling is mediated through five G protein-coupled receptors (S1P1-S1P5). S1P1 is crucial to the exit of T-lymphocytes from the thymus and peripheral lymphoid organs through a gradient of S1P. We have previously observed that T-ALL and T-LBL blasts express S1P1. Herein we analyzed the role of S1P receptors in the migratory pattern of human T-cell neoplastic blasts. S1P-triggered cell migration was directly related to S1P1 expression. T-ALL blasts expressing low levels of S1P1 mRNA (HPB-ALL) did not migrate toward S1P, whereas those expressing higher levels of S1P1 (MOLT-4, JURKAT and CEM) did migrate. The S1P ligand induced T-ALL cells chemotaxis in concentrations up to 500 nM and induced fugetaxis in higher concentrations (1000-10000 nM) through interactions with S1P1. When S1P1 was specifically blocked by the W146 compound, S1P-induced migration at lower concentrations was reduced, whereas higher concentrations induced cell migration. Furthermore, we observed that S1P/S1P1 interactions induced ERK and AKT phosphorylation, and modulation of Rac1 activity. Responding T-ALL blasts also expressed S1P3 mRNA but blockage of this receptor did not modify migratory responses. Our results indicate that S1P is involved in the migration of T-ALL/LBL blasts, which is dependent on S1P1 expression. Moreover, S1P concentrations in the given microenvironment might induce dose-dependent chemotaxis or fugetaxis of T-ALL blasts.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16599/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALcarolina_messias_etal_IOC_2016.pdfcarolina_messias_etal_IOC_2016.pdfapplication/pdf2089682https://www.arca.fiocruz.br/bitstream/icict/16599/2/carolina_messias_etal_IOC_2016.pdf1a0d7c222dd6d80e2d67efd664f2b0a0MD52TEXTcarolina_messias_etal_IOC_2016.pdf.txtcarolina_messias_etal_IOC_2016.pdf.txtExtracted texttext/plain64282https://www.arca.fiocruz.br/bitstream/icict/16599/3/carolina_messias_etal_IOC_2016.pdf.txt6e4ba54773e4e2c0cd04389bd3789f9bMD53icict/165992022-06-24 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dc.title.pt_BR.fl_str_mv Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation
title Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation
spellingShingle Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation
Messias, Carolina V.
Quimiotaxia
Blastos
S1P1
Sphingosine-1-phosphate(S1P)
Chemotaxis
T-ALL Blasts
Fugetaxis
S1P1 Activation
title_short Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation
title_full Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation
title_fullStr Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation
title_full_unstemmed Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation
title_sort Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation
author Messias, Carolina V.
author_facet Messias, Carolina V.
Van-Vliet, Eliane Santana
Lemos, Julia P.
Moreira, Otacilio C.
Almeida, Vinicius Cotta de
Savino, Wilson
Cruz, Daniella Arêas Mendes da
author_role author
author2 Van-Vliet, Eliane Santana
Lemos, Julia P.
Moreira, Otacilio C.
Almeida, Vinicius Cotta de
Savino, Wilson
Cruz, Daniella Arêas Mendes da
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Messias, Carolina V.
Van-Vliet, Eliane Santana
Lemos, Julia P.
Moreira, Otacilio C.
Almeida, Vinicius Cotta de
Savino, Wilson
Cruz, Daniella Arêas Mendes da
dc.subject.other.pt_BR.fl_str_mv Quimiotaxia
Blastos
S1P1
topic Quimiotaxia
Blastos
S1P1
Sphingosine-1-phosphate(S1P)
Chemotaxis
T-ALL Blasts
Fugetaxis
S1P1 Activation
dc.subject.en.pt_BR.fl_str_mv Sphingosine-1-phosphate(S1P)
Chemotaxis
T-ALL Blasts
Fugetaxis
S1P1 Activation
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-12-29T14:17:22Z
dc.date.available.fl_str_mv 2016-12-29T14:17:22Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MESSIAS, Carolina V. et al. Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation. Plos One, v.11, n.1, e0148137, 20p, Jan. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16599
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0148137
identifier_str_mv MESSIAS, Carolina V. et al. Sphingosine-1-Phosphate Induces Dose-Dependent Chemotaxis or Fugetaxis of T-ALL Blasts through S1P1 Activation. Plos One, v.11, n.1, e0148137, 20p, Jan. 2016.
1932-6203
10.1371/journal.pone.0148137
url https://www.arca.fiocruz.br/handle/icict/16599
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/16599/2/carolina_messias_etal_IOC_2016.pdf
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