Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis

Detalhes bibliográficos
Autor(a) principal: Lima, Jhenifer Yonara de
Data de Publicação: 2021
Outros Autores: Santos, Marlon Dias Mariano, Murakami, Mario Tyago, Carvalho, Paulo Costa, Souza, Tatiana de Arruda Campos Brasil de
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/51852
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil.
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spelling Lima, Jhenifer Yonara deSantos, Marlon Dias MarianoMurakami, Mario TyagoCarvalho, Paulo CostaSouza, Tatiana de Arruda Campos Brasil de2022-03-24T13:49:03Z2022-03-24T13:49:03Z2021LIMA, Jhenifer Yonara de et al. Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis. Mem. Inst. Oswaldo Cruz, V. 116, n. e210209, p. 1–7, 2021.1678-8060https://www.arca.fiocruz.br/handle/icict/5185210.1590/0074-02760210209porFundação Oswaldo Cruz. Instituto Oswaldo CruzCross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biorrenováveis. Campinas, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil.Leishmaniasis is a neglected tropical disease caused by the parasite Leishmania braziliensis, commonly found in Brazil and associated with cutaneous and visceral forms of this disease. Like other organisms, L. braziliensis has an enzyme called glutamine synthetase (LbGS) that acts on the synthesis of glutamine from glutamate. This enzyme plays an essential role in the metabolism of these parasites and can be a potential therapeutic target for treating this disease. This study Investigate LbGS structure and generate structural models of the protein. We use the method of crosslinking mass spectrometry (XLMS) and generate structural models in silico using I-TASSER. FINDINGS 42 XLs peptides were identified, of which 37 are explained in a monomeric model with the other five indicating LbGS dimerization and pentamers interaction region. The comparison of 3D models generated in the presence and absence of XLMS restrictions probed the benefits of modeling with XLMS highlighting the inappropriate folding due to the absence of spatial restrictions. In conclusion, we disclose the conservation of the active site and interface regions, but also unique features of LbGS showing the potential of XLMS to probe structural information and explore new drugs.ProteomicsProteinsGlutamate-Ammonia LigaseLeishmania braziliensisProteómicaProteínasGlutamato-Amoníaco LigasaLeishmania braziliensisProtéomiqueProtéinesGlutamate-ammonia ligaseLeishmania brasiliensisProteômicaProteínasGlutamina SintetaseLeishmania braziliensisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/51852/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL1678-8060-mioc-116-e210209-OK.pdf1678-8060-mioc-116-e210209-OK.pdfapplication/pdf1862646https://www.arca.fiocruz.br/bitstream/icict/51852/2/1678-8060-mioc-116-e210209-OK.pdfc1fdcd964111e6c3d1749105200bfb40MD52icict/518522022-03-24 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dc.title.pt_BR.fl_str_mv Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis
title Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis
spellingShingle Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis
Lima, Jhenifer Yonara de
Proteomics
Proteins
Glutamate-Ammonia Ligase
Leishmania braziliensis
Proteómica
Proteínas
Glutamato-Amoníaco Ligasa
Leishmania braziliensis
Protéomique
Protéines
Glutamate-ammonia ligase
Leishmania brasiliensis
Proteômica
Proteínas
Glutamina Sintetase
Leishmania braziliensis
title_short Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis
title_full Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis
title_fullStr Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis
title_full_unstemmed Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis
title_sort Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis
author Lima, Jhenifer Yonara de
author_facet Lima, Jhenifer Yonara de
Santos, Marlon Dias Mariano
Murakami, Mario Tyago
Carvalho, Paulo Costa
Souza, Tatiana de Arruda Campos Brasil de
author_role author
author2 Santos, Marlon Dias Mariano
Murakami, Mario Tyago
Carvalho, Paulo Costa
Souza, Tatiana de Arruda Campos Brasil de
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lima, Jhenifer Yonara de
Santos, Marlon Dias Mariano
Murakami, Mario Tyago
Carvalho, Paulo Costa
Souza, Tatiana de Arruda Campos Brasil de
dc.subject.en.pt_BR.fl_str_mv Proteomics
Proteins
Glutamate-Ammonia Ligase
Leishmania braziliensis
topic Proteomics
Proteins
Glutamate-Ammonia Ligase
Leishmania braziliensis
Proteómica
Proteínas
Glutamato-Amoníaco Ligasa
Leishmania braziliensis
Protéomique
Protéines
Glutamate-ammonia ligase
Leishmania brasiliensis
Proteômica
Proteínas
Glutamina Sintetase
Leishmania braziliensis
dc.subject.es.pt_BR.fl_str_mv Proteómica
Proteínas
Glutamato-Amoníaco Ligasa
Leishmania braziliensis
dc.subject.fr.pt_BR.fl_str_mv Protéomique
Protéines
Glutamate-ammonia ligase
Leishmania brasiliensis
dc.subject.decs.pt_BR.fl_str_mv Proteômica
Proteínas
Glutamina Sintetase
Leishmania braziliensis
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-03-24T13:49:03Z
dc.date.available.fl_str_mv 2022-03-24T13:49:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Jhenifer Yonara de et al. Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis. Mem. Inst. Oswaldo Cruz, V. 116, n. e210209, p. 1–7, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/51852
dc.identifier.issn.pt_BR.fl_str_mv 1678-8060
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760210209
identifier_str_mv LIMA, Jhenifer Yonara de et al. Cross-linking mass spectrometry reveals structural insights of the glutamine synthetase from Leishmania braziliensis. Mem. Inst. Oswaldo Cruz, V. 116, n. e210209, p. 1–7, 2021.
1678-8060
10.1590/0074-02760210209
url https://www.arca.fiocruz.br/handle/icict/51852
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/51852/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/51852/2/1678-8060-mioc-116-e210209-OK.pdf
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repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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