Activities of Psilostachyin A an Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo

Detalhes bibliográficos
Autor(a) principal: Silva, Cristiane França da
Data de Publicação: 2013
Outros Autores: Batista, Denise da Gama Jaen, Araújo, Julianna Siciliano de, Batista, Marcos Meuser, Lionel, Jessica, Souza, Ellen Mello de, Hammer, Erica Ripoll, Silva, Patricia Bernardino da, Miert, Marta de, Adams, Michael, Zimmermann, Stefanie, Hamburger, Matthias, Brun, Reto, Schühly, Wolfang, Soeiro, Maria de Nazaré Correia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11464
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
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spelling Silva, Cristiane França daBatista, Denise da Gama JaenAraújo, Julianna Siciliano deBatista, Marcos MeuserLionel, JessicaSouza, Ellen Mello deHammer, Erica RipollSilva, Patricia Bernardino daMiert, Marta deAdams, MichaelZimmermann, StefanieHamburger, MatthiasBrun, RetoSchühly, WolfangSoeiro, Maria de Nazaré Correia2015-08-19T13:49:15Z2015-08-19T13:49:15Z2013SILVA, Cristiane França da et al. Activities of Psilostachyin A and Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo. Antimicrobial Agents and Chemotherapy, v. 57, n. 11, p. 5307-5314, Nov. 2013.0066-4804https://www.arca.fiocruz.br/handle/icict/1146410.1128/AAC.00595-13engAmerican Society for MicrobiologyMedicamentosTrypanosoma cruziIn VitroIn VivoPsilostachyin ACynaropicrinTécnicas In VitroTrypanosoma cruziActivities of Psilostachyin A an Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.University of Basel. Department of Pharmaceutical Sciences, Pharmaceutical Biology. Basel, Switzerland.University of Basel. Department of Pharmaceutical Sciences, Pharmaceutical Biology. Basel, Switzerland.University of Basel. Department of Pharmaceutical Sciences, Pharmaceutical Biology. Basel, Switzerland / Swiss Tropical and Public Health Institute. Department of Medical Parasitology and Infection Biology. Basel, Switzerland.University of Basel. Department of Pharmaceutical Sciences, Pharmaceutical Biology. Basel, Switzerland.Swiss Tropical and Public Health Institute. Department of Medical Parasitology and Infection Biology. Basel, Switzerland.University of Graz. Institute of Zoology. Graz, Austria.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.In vitro and in vivo activities against Trypanosoma cruzi were evaluated for two sesquiterpene lactones: psilostachyin A and cynaropicrin. Cynaropicrin had previously been shown to potently inhibit African trypanosomes in vivo, and psilostachyin A had been reported to show in vivo effects against T. cruzi, albeit in another test design. In vitro data showed that cynaropicrin was more effective than psilostachyin A. Ultrastructural alterations induced by cynaropicrin included shedding events, detachment of large portions of the plasma membrane, and vesicular bodies and large vacuoles containing membranous structures, suggestive of parasite autophagy. Acute toxicity studies showed that one of two mice died at a cynaropicrin dose of 400 mg/kg of body weight given intraperitoneally (i.p.). Although no major plasma biochemical alterations could be detected, histopathology demonstrated that the liver was the most affected organ in cynaropicrin-treated animals. Although cynaropicrin was as effective as benznidazole against trypomastigotes in vitro, the treatment (once or twice a day) of T. cruzi-infected mice (up to 50 mg/kg/day cynaropicrin) did not suppress parasitemia or protect against mortality induced by the Y and Colombiana strains. Psilostachyin A (0.5 to 50 mg/kg/day given once a day) was not effective in the acute model of T. cruzi infection (Y strain), reaching 100% animal mortality. Our data demonstrate that although it is very promising against African trypanosomes, cynaropicrin does not show efficacy compared to benznidazole in acute mouse models of T. cruzi infection.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSilva_Cristiane_etal_IOC_2013.pdfSilva_Cristiane_etal_IOC_2013.pdfapplication/pdf1332496https://www.arca.fiocruz.br/bitstream/icict/11464/2/Silva_Cristiane_etal_IOC_2013.pdf79ba92fdb2ca5f7771c638b664298568MD52LICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11464/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51icict/114642023-09-04 11:17:49.368oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T14:17:49Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Activities of Psilostachyin A an Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo
title Activities of Psilostachyin A an Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo
spellingShingle Activities of Psilostachyin A an Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo
Silva, Cristiane França da
Medicamentos
Trypanosoma cruzi
In Vitro
In Vivo
Psilostachyin A
Cynaropicrin
Técnicas In Vitro
Trypanosoma cruzi
title_short Activities of Psilostachyin A an Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo
title_full Activities of Psilostachyin A an Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo
title_fullStr Activities of Psilostachyin A an Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo
title_full_unstemmed Activities of Psilostachyin A an Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo
title_sort Activities of Psilostachyin A an Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo
author Silva, Cristiane França da
author_facet Silva, Cristiane França da
Batista, Denise da Gama Jaen
Araújo, Julianna Siciliano de
Batista, Marcos Meuser
Lionel, Jessica
Souza, Ellen Mello de
Hammer, Erica Ripoll
Silva, Patricia Bernardino da
Miert, Marta de
Adams, Michael
Zimmermann, Stefanie
Hamburger, Matthias
Brun, Reto
Schühly, Wolfang
Soeiro, Maria de Nazaré Correia
author_role author
author2 Batista, Denise da Gama Jaen
Araújo, Julianna Siciliano de
Batista, Marcos Meuser
Lionel, Jessica
Souza, Ellen Mello de
Hammer, Erica Ripoll
Silva, Patricia Bernardino da
Miert, Marta de
Adams, Michael
Zimmermann, Stefanie
Hamburger, Matthias
Brun, Reto
Schühly, Wolfang
Soeiro, Maria de Nazaré Correia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Cristiane França da
Batista, Denise da Gama Jaen
Araújo, Julianna Siciliano de
Batista, Marcos Meuser
Lionel, Jessica
Souza, Ellen Mello de
Hammer, Erica Ripoll
Silva, Patricia Bernardino da
Miert, Marta de
Adams, Michael
Zimmermann, Stefanie
Hamburger, Matthias
Brun, Reto
Schühly, Wolfang
Soeiro, Maria de Nazaré Correia
dc.subject.other.pt_BR.fl_str_mv Medicamentos
topic Medicamentos
Trypanosoma cruzi
In Vitro
In Vivo
Psilostachyin A
Cynaropicrin
Técnicas In Vitro
Trypanosoma cruzi
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
In Vitro
In Vivo
Psilostachyin A
Cynaropicrin
dc.subject.decs.pt_BR.fl_str_mv Técnicas In Vitro
Trypanosoma cruzi
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-08-19T13:49:15Z
dc.date.available.fl_str_mv 2015-08-19T13:49:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, Cristiane França da et al. Activities of Psilostachyin A and Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo. Antimicrobial Agents and Chemotherapy, v. 57, n. 11, p. 5307-5314, Nov. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11464
dc.identifier.issn.pt_BR.fl_str_mv 0066-4804
dc.identifier.doi.pt_BR.fl_str_mv 10.1128/AAC.00595-13
identifier_str_mv SILVA, Cristiane França da et al. Activities of Psilostachyin A and Cynaropicrin against Trypanosoma cruzi In Vitro and In Vivo. Antimicrobial Agents and Chemotherapy, v. 57, n. 11, p. 5307-5314, Nov. 2013.
0066-4804
10.1128/AAC.00595-13
url https://www.arca.fiocruz.br/handle/icict/11464
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/11464/2/Silva_Cristiane_etal_IOC_2013.pdf
https://www.arca.fiocruz.br/bitstream/icict/11464/1/license.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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