The OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence

Detalhes bibliográficos
Autor(a) principal: Ristow, Paula
Data de Publicação: 2007
Outros Autores: Bourhy, Pascale, McBride, Flávia Weykamp da Cruz, Figueira, Cláudio Pereira, Huerre, Michel, Ave, Patrick, Girons, Isabelle Saint, Ko, Albert Icksang, Picardeau, Mathieu
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/12132
Resumo: Institut Pasteur. Unité de Biologie des Spirochètes. Paris, France / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Goes. Rio de Janeiro, RJ, Brasil
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spelling Ristow, PaulaBourhy, PascaleMcBride, Flávia Weykamp da CruzFigueira, Cláudio PereiraHuerre, MichelAve, PatrickGirons, Isabelle SaintKo, Albert IcksangPicardeau, Mathieu2015-11-06T13:30:12Z2015-11-06T13:30:12Z2007RISTOW, P. et al. The OmpA-Like protein Loa22 is essential for Leptospiral virulence. Plos Pathogens, v. 3, n. 7, p. e97, 2007.1553-7366https://www.arca.fiocruz.br/handle/icict/1213210.1371/journal.ppat.0030097engPublic Library of ScienceLeptospiroseLeptospiraLeptospira interrogansVirulênciaAnimalHamasterThe OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulenceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, France / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Goes. Rio de Janeiro, RJ, BrasilInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, FranceFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilInstitut Pasteur. Unité de Recherche et d’Expertise Histotechnologie et Pathologie. Paris, FranceInstitut Pasteur. Unité de Recherche et d’Expertise Histotechnologie et Pathologie. Paris, FranceInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, FranceFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Weill Medical College of Cornell University. Division of International Medicine and Infectious Disease. New York, New York, USAInstitut Pasteur. Unité de Biologie des Spirochètes. Paris, FrancePathogenic mechanisms of Leptospira interrogans, the causal agent of leptospirosis, remain largely unknown. This is mainly due to the lack of tools for genetic manipulations of pathogenic species. In this study, we characterized a mutant obtained by insertion of the transposon Himar1 into a gene encoding a putative lipoprotein, Loa22, which has a predicted OmpA domain based on sequence identity. The resulting mutant did not express Loa22 and was attenuated in virulence in the guinea pig and hamster models of leptospirosis, whereas the genetically complemented strain was restored in Loa22 expression and virulence. Our results show that Loa22 was expressed during host infection and exposed on the cell surface. Loa22 is therefore necessary for virulence of L. interrogans in the animal model and represents, to our knowledge, the first genetically defined virulence factor in Leptospira speciesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/12132/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALRistow P The OmpA-like protein....pdfRistow P The OmpA-like protein....pdfapplication/pdf831784https://www.arca.fiocruz.br/bitstream/icict/12132/2/Ristow%20P%20The%20OmpA-like%20protein....pdf47767dc4884db4f525eb74dc93dd0fa1MD52TEXTRistow P The OmpA-like protein....pdf.txtRistow P The OmpA-like protein....pdf.txtExtracted texttext/plain49362https://www.arca.fiocruz.br/bitstream/icict/12132/3/Ristow%20P%20The%20OmpA-like%20protein....pdf.txtc2691e1aa9dba0ae83c66afd68b4dd07MD53icict/121322023-03-15 14:33:23.344oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:23Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence
title The OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence
spellingShingle The OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence
Ristow, Paula
Leptospirose
Leptospira
Leptospira interrogans
Virulência
Animal
Hamaster
title_short The OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence
title_full The OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence
title_fullStr The OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence
title_full_unstemmed The OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence
title_sort The OmpA-Like Protein Loa22 Is Essential for Leptospiral Virulence
author Ristow, Paula
author_facet Ristow, Paula
Bourhy, Pascale
McBride, Flávia Weykamp da Cruz
Figueira, Cláudio Pereira
Huerre, Michel
Ave, Patrick
Girons, Isabelle Saint
Ko, Albert Icksang
Picardeau, Mathieu
author_role author
author2 Bourhy, Pascale
McBride, Flávia Weykamp da Cruz
Figueira, Cláudio Pereira
Huerre, Michel
Ave, Patrick
Girons, Isabelle Saint
Ko, Albert Icksang
Picardeau, Mathieu
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ristow, Paula
Bourhy, Pascale
McBride, Flávia Weykamp da Cruz
Figueira, Cláudio Pereira
Huerre, Michel
Ave, Patrick
Girons, Isabelle Saint
Ko, Albert Icksang
Picardeau, Mathieu
dc.subject.other.pt_BR.fl_str_mv Leptospirose
Leptospira
Leptospira interrogans
Virulência
Animal
Hamaster
topic Leptospirose
Leptospira
Leptospira interrogans
Virulência
Animal
Hamaster
description Institut Pasteur. Unité de Biologie des Spirochètes. Paris, France / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Professor Paulo de Goes. Rio de Janeiro, RJ, Brasil
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2015-11-06T13:30:12Z
dc.date.available.fl_str_mv 2015-11-06T13:30:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RISTOW, P. et al. The OmpA-Like protein Loa22 is essential for Leptospiral virulence. Plos Pathogens, v. 3, n. 7, p. e97, 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/12132
dc.identifier.issn.none.fl_str_mv 1553-7366
dc.identifier.doi.none.fl_str_mv 10.1371/journal.ppat.0030097
identifier_str_mv RISTOW, P. et al. The OmpA-Like protein Loa22 is essential for Leptospiral virulence. Plos Pathogens, v. 3, n. 7, p. e97, 2007.
1553-7366
10.1371/journal.ppat.0030097
url https://www.arca.fiocruz.br/handle/icict/12132
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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